不同生物型BVDV感染宿主细胞后差异基因分析

doi:10.16431/j.cnki.1671-7236.2017.11.001

Abstract

Abstract:

In order to further study the problem of persistent infection and investigate the molecular mechanism of transformation between different biotypes of bovine viral diarrhea virus(BVDV),cytopathic BVDV (CP BVDV) and non cytopathic BVDV (NCP BVDV) were used to infect MDBK host cell and the cells were observed and collected per 12 h from 12 to 72 h,and at the same time the transcriptome analysis were used to analyse the 24 and 48 h MDBK cells.The results showed that at 24 h,compared to MDBK cell infected by NCP BVDV,there were 2 849 up-regulated and 3 347 down-regulated differential genes,while 2 933 up-regulated and 2 831 down-regulated differential genes at 48 h in MDBK cell infected by CP BVDV.The results of GO function classification statistics showed that the molecular function of differential gene mainly involved in catalytic activity,binding activity, enzyme regulator activity,molecular transduction activity and protein binding transcription factor activity. Pathway saliency map showed that the genes involved in autophagy, apoptosis, signal transduction related expression of immunoregulatory factor were preliminary screened. The found of differential gene laid the foundation to further reveal the molecular mechanism of viral pathogenesis,also provided molecular targets for the development of new anti BVDV preparations.

Key words: cytopathogenic BVDV (CP BVDV); non cytopathic BVDV (NCP BVDV); differential gene; GO function analysis; Pathway analysis

CLC Number:

S855.3

CHENG Kai-hui, ZHU Tong, HOU Pei-li, WAMG Hong-mei, MIAO Zi-li, ZHANG Liang, XIE Xiao-li, YANG Hong-jun, HE Hong-bin. Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus[J]. , 2017, 44(11): 3113-3120.

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Differential Gene Analysis of Host Cell Infected by Different Biotypes of Bovine Viral Diarrhea Virus

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