干扰lnc23后牛骨骼肌卫星细胞转录组测序的生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.04.001

Abstract

Abstract: This study was aimed to analyze the changes of transcriptional group level of a muscle highly expressed and functional lncRNA (lnc23),and screen out the regulatory pathways and related mRNAs,which might be involved in regulating the growth of skeletal muscle satellite cells.The second generation sequencing technique (next-generation sequencing,NGS) was used to sequence the successfully constructed lnc23 inhibition model and the corresponding control group of bovine skeletal muscle satellite cells based on Illumia hiSeq sequencing platform.After sorting,filtering and evaluating the data,the transcriptional differentially expressed genes between the samples were analyzed by bioinformatics,and the differential genes were analyzed by GO and KEGG enrichment methods.The results of the transcriptome data were verified by Real-time quantitative PCR.The results showed that 19 358 genes were sequenced and 1 297 differential expression genes were screened,of which 856 genes were up-regulated,and 441 genes were down-regulated.The GO function of the differential genes,including the cellular components,the molecular function and the biological process,were divided into 3 categories (222 branches),among which a large number of differential genes were related to the catalytic activity,the molecular function regulation,the signal transduction,the biological adhesion and the metabolism.KEGG analysis results showed that the differential genes were involved in 190 pathways,which were significantly enriched in PI3K-Akt,P53,TNF,RIG-Ⅰ-like receptors,neuroactive ligand receptor interactions,cytokine interactions and other signaling pathways,and further screened out differential genes,such as CCNA2,RRM2,IL-6 and MYL2,which might be involved in cell growth and muscle development.Real-time quantitative PCR results showed that 9 of 11 differential genes were consistent with the sequencing results of the transcriptional group,indicating the reliability of the sequencing results.This study successfully finished the interference with lnc23 and its control,completed the transcriptional group sequencing analysis of bovine skeletal muscle satellite cells,obtained the functional annotation information of differential genes and preliminarily revealed the potential genes and pathways of lnc23 regulating the differentiation of bovine skeletal muscle satellite cells,which laid a good foundation for further exploring the differentiation mechanism of bovine skeletal muscle satellite cells regulated by lnc23.

Key words: lncRNA; bovine skeletal muscle; satellite cell; transcriptome; signaling pathway; differential genes

CLC Number:

SONG Yingshen, GUO Yiwen, MIAO Manning, ZHANG Linlin, LI Xin, DING Xiangbin, GUO Hong. Bioinformatics Analysis of Transcriptome Sequencing of Bovine Skeletal Muscle Satellite Cells After Interference with lnc23[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 973-983.

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Bioinformatics Analysis of Transcriptome Sequencing of Bovine Skeletal Muscle Satellite Cells After Interference with lnc23

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