高温胁迫下罗非鱼源无乳链球菌的比较转录组分析

doi:10.16431/j.cnki.1671-7236.2023.06.004

Abstract

Abstract: 【Objective】 The purpose of this study was to understand the causes of the outbreak of Streptococcus agalactis (S.agalactis) disease in high temperature season at the molecular level.【Method】 S.agalactis was cultured at 35 and 28 ℃ respectively, and total RNA was extracted for transcriptome sequencing.The differentially expressed genes(DEGs) were analyzed by Dr Tom's analysis platform of BGI, and the classification and enrichment of KEGG pathway and GO function were carried out.The reliability of transcriptome data was determined by randomly detecting the differential expression of 10 genes by Real-time quantitative PCR.【Result】 A total of 357 DEGs were obtained under 35 and 28 ℃, including 229 up-regulated genes and 128 down-regulated genes.The enrichment of GO database could obtain multiple functional sets with significant, most of which focus on the pathways related to carbon source metabolism, such as carbohydrate catabolic process, glycogen metabolic process, cellular glucan metabolic process, etc.. Many genes related to the pathogenicity of S.agalactis were found among the DEGs, such as the coding genes of alkyl hydroperoxide reductase subunit, CAMP factor, C5a peptidase, etc.. The Pearson correlation coefficient R value between Real-time quantitative PCR and transcriptome data was 0.979 (P<0.001), indicating that the transcriptome data was reliable and effective.【Conclusion】 High temperature could significantly affect the pathway related to the carbon source metabolism of S.agalactis, which might promote the growth and diffusion of the cell by accelerating the use of carbon source.High temperature also induced the up-regulation of several genes related to pathogenicity.Among them, cell membrane lysis caused by cAMP factor and immune escape caused by oxidoreductases might be the main factors that enhance the pathogenicity of S.agalactis under high temperature.

Key words: tilapia; Streptococcus agalactiae; transcriptome sequencing; cAMP factor; alkyl hydrogen peroxide reductase

CLC Number:

S852

SHI Jingu, HUANG Shanshan, HUANG Jiwei, CHEN Kunhai, HU Dasheng, ZHU Zhixiong, HAN Shuyu. Comparative Transcriptome Analysis of Streptococcus agalactiae from Tilapia Under High Temperature Stress[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2207-2216.

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Comparative Transcriptome Analysis of Streptococcus agalactiae from Tilapia Under High Temperature Stress

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