贞苓增免散对罗非鱼的安全性评价

doi:10.16431/j.cnki.1671-7236.2021.11.010

Abstract

Abstract: The purpose of the trial was to determine the safety of Zhenling Zengmian powder on tilapia at the recommended clinical dose, study the safe range of tilapia dose and comprehensively evaluate the efficacy of the drug, so as to provide important basis and guidance for clinical rational drug use. Five groups were set up in this experiment, including blank group (basic feed), 1 time dose group (including 0.4% of Zhenling Zengmian powder), 3 times dose group (including 1.2% of Zhenling Zengmian powder), 5 times dose group (including 2.0% of Zhenling Zengmian powder), and 10 times dose group (including 4.0% of Zhenling Zengmian powder). The tilapia were fed continuously for 30 days, and the clinical signs, blood physiological and biochemical indexes, organ index and histopathology of tilapia were detected on the 15th and 30th day, respectively. The results showed that the activity, feeding, body color and excretion of tilapia in each dose group were good, which was not significantly different from that in the blank group. The weight gain rate and specific growth rate were the highest in the 1 time dose group, which were significantly or extremely significantly higher than those in the blank group (P<0.05;P<0.01), but decreased with the increase of the amount of Zhenling Zengmian powder from 0 to 15 days. From 15 to 30 days, the weight gain rate of each dose group of Zhenling Zengmian powder was significantly higher than that of the blank group (P<0.01). Some of the blood physiological and biochemical indexes of tilapia in each dose group were significantly different from those in the blank group, but they were all within the normal range. The tilapia spleen index of each dose group at 0-15 days and the 1 time dose group at 15-30 days were significantly higher than that of the blank group (P<0.05). The liver and spleen sections of tilapia in each group were observed under microscope, and no cytotoxic changes caused by drugs were found. The above results showed that Zhenling Zengmian powder was safe to feed tilapia for continuously 30 days at or below the 10 times of recommended dose. As a feed additive, it could improve the growth performance and enhance immunity of tilapia, which was of great significance to promote the rapid and efficient development of tilapia culture.

Key words: Zhenling Zengmian powder; tilapia; physiological index; biochemical index

CLC Number:

S948

HE Wenna, XIAO Ting, HE Ying, ZHOU Yingning, JIANG Dongfu, LU Jingzhuan, CHEN Zhongwei, HU Tingjun. Safety Evaluation of Zhenling Zengmian Powder on Tilapia[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4004-4013.

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Safety Evaluation of Zhenling Zengmian Powder on Tilapia

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