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Identification and Function Prediction Analysis of circ_CSPP1 in Ovarian Tissues of Xiang Pigs
- HUANG Yali, NIU Xi, LU Huan, HUANG Shihui, WANG Jiafu, RAN Xueqin, LI Sheng
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2021, 48(11):
4055-4066.
doi:10.16431/j.cnki.1671-7236.2021.11.015
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Abstract
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The purpose of this study was to explore the potential functions of circ_CSPP1 in the ovarian tissues of Xiang pigs. In this study, the sequence of circ_CSPP1 was spliced by CIRI-full, the conservative analysis of circ_CSPP1 was calculated by online blat of circBase database, and the characteristics of circ_CSPP1 in the ovarian tissues of Xiang pigs were identified and analyzed by RT-PCR technology and Sanger sequencing technology. Moreover, miRanda, RNAhybrid and PITA softwares were used to predict and analyze the circ_CSPP1 bound miRNA molecular and miRNA target genes. Then GO and KEGG enrichment analyses were performed for the target genes. The results showed that the isolated circ_CSPP1 was conservative and originated from exons 8-12 of CSPP1 gene, which could be served as molecular sponges of 14 miRNAs, such as ssc-miR-324, ssc-miR-10a-5p, ssc-miR-9847-3p, ssc-miR-365-5p, ssc-miR-92b-5p, ssc-miR-885-3p, ssc-miR-7139-3p, ssc-miR-9851-3p, ssc-miR-33b-3p etc, and targeted 755 protein genes. GO enrichment analysis showed that the target genes of circ_CSPP1 were significantly enriched in 153 GO terms, in which cell maturation, cell division sites, negative regulation of cell migration, Wnt signaling pathway, cyclin binding, uterine development, activation of MAPK activity, focal adhesion and cleavage groove were related to ovarian function. KEGG enrichment analysis showed that the target genes of circ_CSPP1 were enriched into 260 signal pathways, of which 29 signal pathways were significantly enriched, and the target genes mainly concentrated in cancer pathway, cell cycle, estrogen signaling pathway, PI3K-AKT signaling pathway, ovarian steroidogenesis, oxytocin pathway and apoptosis etc. In conclusion, circ_CSPP1 might act as molecular sponges of many miRNAs to regulate the biological processes including proliferation and apoptosis of ovarian granulosa cells, follicle maturation, ovulation process, ovarian steroidogenesis and premature ovarian failure in the ovarian tissues of Xiang pigs.