贵州省三穗县鸭疫里默氏杆菌的分离鉴定、毒力基因检测及耐药性分析

doi:10.16431/j.cnki.1671-7236.2021.11.037

Abstract

Abstract: In order to understand the epidemic serotype, virulence and drug resistance of Riemerella anatipestifer (RA) in Sansui county, Guizhou province, 6 isolates were isolated from clinically suspected RA infected ducks in six large-scale duck farms in Sansui county, Guizhou province. The pathogenic bacteria were isolated and identified, and virulence gene detection and drug resistance analysis were carried out. The results of bacterial isolation and identification showed that the isolates were smooth, round and translucent drop-shaped colonies on chocolate agar medium. Gram staining was negative for short bacilli, Wright staining showed bipolar staining. The isolates did not have movement. The test of urea, catalase and oxidase were positive, which was in line with the biochemical characteristics of RA. The 6 strains of isolates were named SS-RA1 to SS-RA6. The 16S rRNA gene sequence of the 6 strains of isolates was compared with that of the RA reference strain on NCBI, the gene sequence similarity was ≥ 98%, indicating that all 6 strains were RA. SS-RA1 to SS-RA4 were serotype 2 and contain 8 virulence genes (OmpA、CAMP、wza、AS87_04050、Fur、SIP、TbdR1 and luxE genes), SS-RA5 and SS-RA6 were serotype 11, lacking the AS87_04050 gene, and only containing the remaining 7 virulence genes. The results of animal regression test showed that all the ducklings in the virus attack group died, and the ducklings in the control group did not show obvious clinical symptoms, indicating that the 6 strains of bacteria were pathogenic to the ducklings. The results of drug sensitivity test showed that the 6 strains of isolates were only sensitive to 7 antibiotics:Carboxybenzyl, piperacillin, ceftazidime, cefalexin, ceftriaxone, cefradine and cefoperazone, and showed different degrees of resistance to the other 13 antibiotics. The resistance rates to aminoglycosides and macrolides were 100%. In this study, the 6 RA strains were successfully isolated, providing a theoretical basis for vaccine selection and drug control of Riemerella anatipestifer infection in Sansui county, Guizhou province.

Key words: Riemerella anatipestifer; isolation and identification; virulence genes; antibiotic resistance analysis

CLC Number:

S852.61

WU Zhengzhuo, CHEN Guoquan, YAO Biqiong, SHI Dajun, YAN Chaohua, WANG Na, WANG Kaigong, ZHOU Bijun, CHENG Zhentao, WEN Ming. Isolation, Identification, Virus Gene Detection and Antibiotic Resistance Analysis of Riemerella anatipestifer in Sansui County of Guizhou Province[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4274-4283.

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Isolation, Identification, Virus Gene Detection and Antibiotic Resistance Analysis of Riemerella anatipestifer in Sansui County of Guizhou Province

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