鸭疫里氏杆菌二价灭活疫苗制备及免疫效力研究

doi:10.16431/j.cnki.1671-7236.2023.01.029

Abstract

Abstract: 【Objective】 The purpose of the test was to prepare bivalent inactivated vaccine according to Guizhou epidemic strains of Riemerella anatipestifer (RA) serotype 1 and 2, and provide research data for the prevention and control of RA infection and vaccine development.【Method】 The local epidemic strains of serotype 1 Riemerella anatipestifer (RA-G06) and serotype 2 RA (RA-HS01) were used as strains, and the growth curves of the strains were determined by the smear method and the modified Coulomb method was used to calculate the median lethal dose(LD₅₀) of the strains to ducks.After culturing to a final concentration of 1×10¹⁰ CFU/mL, the two strains were mixed in equal proportions, and then the bivalent inactivated vaccine was prepared with carbomer as an adjuvant, which was used to immunize ducks after the vaccine quality inspection.The protection rate of the vaccine was evaluated by detecting the level of specific antibodies in the sera of the immunized ducks and the challenge protection test, and the histopathology of the heart, liver, spleen and brain tissues of the ducks in the challenge test were observed.【Result】 RA-G06 strain and RA-HS01 strain both reached the peak value after 12 h of culture, the numbers of viable bacteria were 2.1×10¹¹ and 3.3×10¹¹ CFU/mL, LD₅₀ were 1.44×10¹⁰and 2.63×10⁸ CFU/mL, respectively.The prepared vaccine was safe and the humoral immunity could be induced in ducks.The results of RA-G06 strain challenge protection test showed that the protection rates of the self-developed vaccine and commercial vaccine were both 90%.The results of the challenge test of RA-HS01 showed that the protection rate of the self-developed vaccine was 90%, and the protection rate of the commercial vaccine was 80%.Histopathological results showed that after immunization, each vaccine group could provide better protection to duck heart, liver, spleen and brain tissues, and the self-developed vaccine group had obvious advantages.【Conclusion】 It showed that the carbomer adjuvant inactivated vaccine prepared by using RA serotype 1 and 2 strains circulating in Guizhou had obvious immune effect, and could play an important role in the prevention and control of RA infection in Guizhou.

Key words: Riemerella anatipestifer (RA); immune protection; inactived vaccine

CLC Number:

S859.79^＋7

XU Jinge, LI Ting, CHEN Rujia, YU Bo, ZHANG Yanan, YANG Li, ZHAO Bin, PU Ling. Preparation and Immune Efficacy of Riemerella anatipestifer Bivalent Inactivated Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 290-299.

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Preparation and Immune Efficacy of Riemerella anatipestifer Bivalent Inactivated Vaccine

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