China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (7): 2716-2724.doi: 10.16431/j.cnki.1671-7236.2022.07.030

• Preventive Veterinary Medicine • Previous Articles     Next Articles

Preparation and Immunological Study of Inactived Vaccine of Type A: L1 Pasteurella multocida from Ducks

TIAN Yujie1, LI Ting1, DAI Guotao1, PU Ling1, ZHANG Yanan1, YUAN Chao1, YANG Maosheng1, CHEN Qiang2, LI Meijuan1, XU Jinge1   

  1. 1. Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang 550005, China;
    2. Institute of Pratacultural, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China
  • Received:2022-02-22 Online:2022-07-05 Published:2022-06-29

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Abstract: 【Objective】 The inactivated Pasteurella multocida vaccine was prepared to effectively control the occurrence and prevalence of Pasteurella multocida in ducks in Guizhou province.【Method】 The local epidemic strain of A:L1 type Pasteurella multocida,isolated and preserved in the laboratory,was used as the strain.The growth curve of the strain was determined by the plating method and the median lethal dose (LD50) was calculated by improved Karber's method.After the bacteria were cultured to a final concentration of 1×1010 CFU/mL,the oil adjuvant inactivated vaccine and carbomer adjuvant inactivated vaccine were prepared respectively,and then the immunized duckling test was conducted after the vaccine's quality was inspected.The protection rate of the two vaccines were evaluated and compared by the challenge protection test,and the histopathological observation was performed on the liver,lung and spleen of the ducks in the challenge test.【Result】 The vaccine strain of A:L1 type Pasteurella multocida reached to the higestaed after 18 h,the concentration of viable bacteria was reached to 1.0×1010 CFU/mL,and the LD50 was reached to 5 CFU.The safety of the two vaccines was qualified.The challenge protection test results showed that after first immunization,the immune protection rate of the carbomer adjuvant inactivated vaccine could reached 62.5%,which was higher than that of the oil adjuvant inactivated vaccine (50.0%) and the commercial inactivated vaccine (50.0%).After the second immunization,carbomer adjuvant inactivated vaccine had obvious advantages,and the immune protection rate could reached 87.5%,which was higher than that of oil adjuvant inactivated vaccine (75.0%) and commercial inactivated vaccine (62.5%).The histopathological results showed that carbomer adjuvant inactivated vaccine could provide best protective effects on the lung after the first immunization,and in terms of the protective effects of liver,lung and spleen after the second immunization,the carbomer adjuvant inactivated vaccine group had obvious advantages.【Conclusion】 The carbomer adjuvant inactivated vaccine prepared by using the A:L1 strains prevalent in Guizhou had obvious immune effects,which could play an important role in the prevention and control of Pasteurellosis multocida in Guizhou.

Key words: duck Pasteurella multocida; type A:L1; immune protection; inactived vaccine

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