猪伪狂犬病病毒灭活疫苗对猪伪狂犬病病毒流行毒株和经典毒株的免疫保护效果评估

doi:10.16431/j.cnki.1671-7236.2021.04.027

Abstract

Abstract: In order to evaluate of the protective efficacy of Pseudorabies virus (PRV) inactivated vaccine (HN1201-ΔgE) against the challenge of PRV epidemic and classic strains,piglets were immunized with the inactivated vaccine (HN1201-ΔgE) and live vaccine (Bartha-K61) separately and the gB antibody (Ab) was evaluated using the serum samples collected pre-immune and 7,10,14,17,21,24 and 28 days post-immunization.The pigs were challenged with the epidemic strain of HN1201 and the classical strain of Fa at the 28th day post-immunization,respectively.After challenging,the body temperature,gE antibody (7 and 14 days post-challenge),and the virus shedding (0-8 days post-challenge) were tested.The results showed that the production of gB Ab in HN1201-ΔgE immunization group was earlier and higher than that in Bartha-K61 immunization group.Compared with control group,the appearance of gE Ab in all immunization groups postponed and showed a lower rate of virus shedding after challenge.The control group showed typical clinical symptoms and pathological damage after challenge,together with the positive result of antigen detection in IHC test,while the piglets in the two immunization groups had no obvious clinical symptoms,and the IHC test was negative,except part of the organs in the Bartha-K61 immunization group had minor pathological damage.Compared with control group,the pigs in two immunization groups showed a late seroconversion of gE antibody.The gE antibody level of the HN1201-ΔgE immunization group was lower than that of the Bartha-K61 immunization group.The Bartha-K61 immunization group showed virus shedding on the 3rd to 5th day post challenge.While no virus shedding was detected in the HN1201-ΔgE immunization group.Our studies suggested that the inactivated vaccine (HN1201-ΔgE strain) provided complete protection against the challenge of both the PRV epidemic and classical strains.

Key words: porcine pseudorabies; inactivated vaccine; pandemic strain; classic strain; immune protection

CLC Number:

S852.4

WANG Yuzhou, XIE Limin, GUO Linghua, BAI Xiaofei, SUN Zhe, HUANG Baicheng, XIAO Yan, TIAN Kegong. Efficacy Evaluation of Pseudorabies Virus Inactivated Vaccine Against the Challenge of the Epidemic and Classic Strains[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1396-1404.

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Efficacy Evaluation of Pseudorabies Virus Inactivated Vaccine Against the Challenge of the Epidemic and Classic Strains

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