VA5免疫增强剂对鸽新城疫病毒灭活疫苗的增效作用研究

doi:10.16431/j.cnki.1671-7236.2020.12.027

Abstract

Abstract: This study was designed to evaluate the synergistic effect of VA5 immunopotentiator on pigeon ND4416 inactivated vaccine.160 healthy pigeons were randomly divided into four groups,including ND4416 strain inactivated vaccine group (NDV group),ND4416 strain inactivated vaccine and immunopotentiator (VA5) mixed group (NDV+VA5 group),La Sota inactivated vaccine group (La Sota group) and normal saline as the control group (C group),to assess their vaccine efficacy against virulent pigeon NDV by serological analysis and animal testing.Pigeons sera were collected at different time points after immunization and measured the HI antibody titer of each group.The results showed that VA5 immunopotentiator significantly improved the serum antibody level of pigeons immunized with pigeon Newcastle disease inactivated vaccine (P<0.05).In addition,comparative test of spleen lymphocyte transformation was conducted at various time points after immunization.The results indicated that VA5 effectively stimulated the lymphocyte transformation of immune pigeon.Pigeons in each groups were challenged with ND4416 strain at the 30th,90th and 180th d after immunization.The results presented that the NDV+VA5 group had 100% protection rate and higher than La Sota group.The duration of immunization test showed that the antibody titer of NDV+VA5 group reached peak 11.20log₂ at the 21st d,remained 7.50log₂ at 180th d,and the protection rate remained 100% at 180th d.It indicated that VA5 immunopotentiator sustained the immune duration of pigeon NDV vaccine up to 180 d.Moreover,the in vitro detoxification test results suggested that VA5 immunopotentiator reduced the in vitro detoxification cycle of pigeons after challenge.Overall,this study suggested VA5 immunopotentiator could significantly improve the immune efficacy of pigeon Newcastle disease inactivated vaccine,which provided a basis for further enhancing the efficacy of Newcastle disease vaccine,as well increased experimental data for the application of immunopotentiators.

Key words: pigeon; Newcastle disease virus inactivated vaccine; ND4416 strain; VA5 immunopotentiator; immune efficacy

CLC Number:

S852.65⁺9.5

XIONG Xiaoyan, QIN Zhenbin, MO Hongfang, HE Dongxian, YU Lintian, XU Jiarong. Study on Synergistic Effect of VA5 Immunopotentiator on Pigeon NDV Inactivated Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4032-4040.

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Study on Synergistic Effect of VA5 Immunopotentiator on Pigeon NDV Inactivated Vaccine

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