新生水貂肠炎病毒母源抗体消长规律及其亚单位疫苗免疫效果研究

doi:10.16431/j.cnki.1671-7236.2020.11.034

Abstract

Abstract: The purpose of this study was to investigate the dynamic of mink enteritis virus (MEV) maternal antibody and the immune effects of subunit vaccines.The offspring of immunized female mink were studied,serum of 21,30,45 and 60 days old mink were collected to determine the HI titer of maternal antibody of MEV.25 healthy minks aged 47~52 days were selected to be inoculated with MEV genetic engineering subunit vaccine,and then blood samples were collected 14 d before and after immunization to determine MEV HI titer.The clinical symptoms and the titer of feces HA of mink enteritis virus were observed 14 d after immunization.Dying and surviving minks were euthanized 14 d after challenge,the duodenum,jejunum and ileum were collected for histopathological observation and immunohistochemical detection.The results showed that the MEV HI of the offspring of immunized female mink decreased gradually with the increase of day age,it was higher at 21 d of age,the MEV HI titer of some minks at 45 d was <1:32 and ≤ 1:4 at 60 d.The MEV HI titer of the control mink was not higher than 1:4 on 14 d after the preparation of qualified vaccine,while it was increased to 1:64~1:512 in immune group.Challenge protection tests showed that the immuned minks were 100% resistant to the attack of mink enteritis virus,and there was no abnormality in the mink's mental state,diet and feces,the HA titer of feces matter after challenge was 1:8~1:16.Histopathological and immunohisto-chemical tests showed that MEV genetic engineering subunit vaccine could well prevent the replication of virus in intestinal mucosal epithelial cells and the damage to intestinal epithelial cells.Therefore,the antibody titer was highest at 21 day-old after immunizing female mink,and the prepared vaccine could break through maternal antibody interference and produce high levels of antibodies when it immunized minks at about 50 d of age,and could resist the attack of mink enteritis virus virulent strain.

Key words: mink viral enteritis; dynamic of maternal antibody; vaccine; immune efficacy; challenge test

CLC Number:

S858.92

WU Hongchao, WANG Lingxiao, HUO Ningning, DING Hangtian, CHEN Yalei, XI Xiangfeng, LIU Youcai, LIU Yuxiu, TIAN Kegong. The Dynamic of Maternal Antibody Against Enteritis Virus in Newborn Minks and Immune Efficacy of the Subunit Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3705-3712.

References

[1] ZHANG Q M,WANG Y P,JI Q,et al.Selection of antiviral peptides against mink enteritis virus using a phage display peptide library[J].Current Microbiology,2013,66(4):379-384.
[2] REYNOLDS H A.Some clinical and hematological features of virus enteritis of mink[J].Canadian Journal of Comparative Medicine Revue Canadienne De Medecine Comparee,1969,33(2):155.
[3] HUNDT B,BEST C,SCHLAWIN N,et al.Establishment of a mink enteritis vaccine production process in stirred-tank reactor and wave bioreactor microcarrier culture in 1-10 L scale[J].Vaccine,2007,25(20):3987-3995.
[4] WANG J K,CHENG S P,YI L,et al.Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP)[J].Journal of Virological Methods,2013,187(2):401-405.
[5] 郭万里.水貂肠炎细小病毒二联灭活疫苗的研制[D].泰安:山东农业大学,2019. GUO W L.Development of divalent inactivated vaccine for mink enteritis parvovirus[D].Tai'an:Shandong Agricultural University,2019.(in Chinese)
[6] 朱翔宇,鲁荣光,王洋,等.水貂细小病毒MEV-LT18株的分离鉴定及遗传进化分析[J].中国兽医学报,2020,40(2):278-284. ZHU X Y,LU R G,WANG Y,et al.Isolation,identification and phylogenetic analysis of mink enteritis virus MEV-LT18[J].Chinese Journal of Veterinary Science,2020,40(2):278-284.(in Chinese)
[7] 王洋.水貂肠炎细小病毒在体内分布规律及相关细胞因子的变化[D].北京:中国农业科学院,2016. WANG Y.Cytokines changes and viral distribution in vivo of mink infection with mink enteritis virus[D].Beijing:Chinese Academy of Agricultural Sciences,2016.(in Chinese)
[8] 左静.水貂肠炎细小病毒抗原性分析及其特异性马源抗体的制备[D].长春:吉林大学,2011. ZUO J.Antigen analysis of mink enteritis parvovirus and preparation of specific horse antibodies against mink enteritis parvovirus[D].Changchun:Jilin University,2011.(in Chinese)
[9] 刁非非.水貂肠炎细小病毒分离株的分子生物学特性及其致病性研究[D].泰安:山东农业大学,2017. DIAO F F.Molecular characterization of mink enteritis virus isolated from mink and its pathogenesis in mink[D].Tai'an:Shandong Agricultural University,2017.(in Chinese)
[10] 孟晓琴,曹世祯,卓春花,等.母源抗体对猪瘟疫苗免疫效果的影响[J].中国兽医杂志,2013,49(1):40-41. MENG X Q,CAO S Z,ZHUO C H,et al.The effect of maternal antibody on the immune efficacy of classical swine fever vaccine[J].Chinese Journal of Veterinary Medicine,2013,49(1):40-41.(in Chinese)
[11] 程世鹏,吴威,闫喜军.毛皮动物主要传染病免疫失败原因分析及防制措施[J].特产研究,2004,4:46-49. CHENG S P,WU W,YAN X J.The analysis of immunological defeat reason and measurement of prevention and control on the major infectious diseases of fur animal[J].Special Wild Economic Animal and Plant Research,2004,4:46-49.(in Chinese)
[12] 夏业才,陈光华,丁家波.兽医生物制品学[M].北京:中国农业出版社,2018. XIA Y C,CHEN G H,DING J B.Science of Veterinary Biologicals[M].Beijing:China Agriculture Press,2018.(in Chinese)
[13] JIN H L,XIA X Z,LIU B,et al.High-yield production of canine parvovirus virus-like particles in a baculovirus expression system[J].Archives of Virology,2016,161(3):705-710.
[14] 张评浒,任涛,唐应华,等.免疫程序与母源抗体对H5禽流感疫苗免疫鸭和鹅HI抗体水平的影响[J].中国兽医科技,2005,8:57-61. ZHANG P H,REN T,TANG Y H,et al.Influence of immunization programs and maternal antibody upon HI antibody levels of ducks and geese immunized with H5 AIV vaccines[J].Chinese Journal of Veterinary Science and Technology,2005,8:57-61.(in Chinese)
[15] 王洋,牛登云,刘昊,等.水貂细小病毒NS1与VP2蛋白的原核表达及免疫原性分析[J].中国畜牧兽医,2016,43(6):1630-1634. WANG Y,NIU D Y,LIU H,et al.Prokaryotic expression and immunogenicity analysis of mink parvovirus NS1 and VP2 protein[J].China Animal Husbandry & Veterinary Medicine,2016,43(6):1630-1634.(in Chinese)
[16] DALSGAARD K,UTTENTHAL S,JONES T D,et al.Plant-derived vaccine protects target animals against a viral disease[J].Nature Biotechnology,1997,15(3):248-252.
[17] LANGEVELD J P M,KAMSTRUP S,UTTENTHAL A,et al.Full protection in mink against mink enteritis virus with new generation canine parvovirus vaccines based on synthetic peptide or recombinant protein[J].Vaccine,1995,13(11):1033-1037.
[18] KOST T A,KEMP C W.Fundamentals of baculovirus expression and applications[J].Advances in Experimental Medicine and Biology,2016,896:187-197.
[19] CHRISTENSEN J,ALEXANDERSEN S,BLOCH B,et al.Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system:A recombinant vaccine for mink enteritis parvovirus in mink[J].Journal of General Virology,1994,75(1):149-155.
[20] 倪佳.表达水貂肠炎病毒VP2蛋白的重组杆状病毒的构建及其免疫原性分析[D].北京:中国农业科学院,2011. NI J.Generation of recombinant baculovirus expressing VP2 protein of mink enteritis virus and analysis of its immunogenicity in a rabbit model[D].Beijing:Chinese Academy of Agricultural Sciences,2011.(in Chinese)
[21] 倪佳,张蕾,柴秀丽,等.水貂肠炎病毒VP2基因在昆虫杆状病毒表达系统中的表达[J].中国畜牧兽医,2011,38(9):97-100. NI J,ZHANG L,CHAI X L,et al.Expression of VP2 gene of mink enteritis virus in baculovrius expression system[J].China Animal Husbandry & Veterinary Medicine,2011,38(9):97-100.(in Chinese)
[22] 谭婷婷.水貂细小病毒的分离鉴定及卵黄抗体的制备[D].哈尔滨:东北林业大学,2010. TAN T T.Isolation,identification of mink enteritis virus and preparation of immunoglobulin[D].Harbin:Northeast Forestry University,2010.(in Chinese)
[23] MAO Y,WANG J,HOU Q,et al.Comparison of biological and genomic characteristics between a newly isolated mink enteritis parvovirus MEV-LHV and an attenuated strain MEV-L[J].Virus Genes,2016,52(3):388-396.
[24] 谭斌,陈微晶,武华.致病性水貂肠炎病毒MEV-ZJ1株的分离与鉴定[J].中国畜牧兽医,2011,38(2):149-151. TAN B,CHEN W J,WU H.Isolation and identification of pathogenic mink enteritis virus MEV-ZJ1 strain[J].China Animal Husbandry & Veterinary Medicine,2011,38(2):149-151.(in Chinese)
[25] REYNOLDS H A.Pathological changes in virus enteritis of mink[J].Canadian Journal of Comparative Medicine Revue Canadienne De Medecine Comparee,1970,34(2):155-163.

The Dynamic of Maternal Antibody Against Enteritis Virus in Newborn Minks and Immune Efficacy of the Subunit Vaccine

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