鸭疫里默氏杆菌贵州流行株蜂胶灭活疫苗制备

doi:10.16431/j.cnki.1671-7236.2022.08.032

Abstract

Abstract: 【Objective】 This study was aimed to prepare propolis inactivated vaccine of Riemerella anatipestifer (RA) epidemic strain in Guizhou.【Method】 The RA serotype 2 Guizhou epidemic strain (RA-SS-8 strain) was selected as the basic strain,and the bacterial growth curve was measured by spectrophotometry and plate counting method in turn,and then the inactivation conditions were screened.Propolis was used as an adjuvant to prepare RA Guizhou epidemic strain propolis inactivated vaccine,and conduct sterility test,safety test and immune duck challenge protection test were carried out.【Result】 Spectrophotometric determination of D₆₀₀_nm value of strain RA-SS-8 with culture time showed that the bacteria proliferated slowly in the period of 0-3 h,and the proliferation trend was obviously accelerated in the period of 3-10 h,and the proliferation gradually became flat after 10 h,with the extension of the culture time,the bacteria finally entered the decay stage.The results of plate counting method showed that when the D_{600 nm} value of the RA-SS-8 strain was 0.1-0.8,the bacteria was in the logarithmic growth phase,and the D_{600 nm} value and the number of viable bacteria showed a good linear relationship. The best inactivation conditions of RA-SS-8 were 0.2% formaldehyde solution and 37 ℃ for 12 h.The bacterial content of the propolis inactivated vaccine was 3.8×10⁹ CFU/mL,and the dry matter content of propolis was 10 mg/mL.No colony growth was found on the chocolate agar medium in the sterility test.Ducks inoculated with 2 times of the immunization dose did not show adverse reactions during the observation period,and no obvious lesions were observed in gross lesions.The protection test of immunized ducks showed that the protection rate of ducks in the immunized group against RA-SS-8 strain was 70%,the propolis inactivated vaccine had good protective effect on the heart and liver tissues of the experimental ducks.【Conclusion】 The propolis inactivated vaccine of RA epidemic strain in Guizhou was successfully prepared,which laid a foundation for the preparation of propolis inactivated vaccine and animal immunity test.

Key words: Riemerella anatipestifer; propolis inactivated vaccine; challenge protection test

CLC Number:

S852.61

MA Guangqiang, LIU Lijuan, CHEN Guoquan, WU Liangtao, YANG Jun, LIU Junze, LI Xiaomeng, PAN Chengwen, ZHOU Bijun. Preparation of Propolis Inactivated Vaccine of Riemerella anatipestifer Epidemic Strain in Guizhou[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3163-3170.

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Preparation of Propolis Inactivated Vaccine of Riemerella anatipestifer Epidemic Strain in Guizhou

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