番鸭源鸭疫里默氏杆菌的分离鉴定及其耐药性分析

doi:10.16431/j.cnki.1671-7236.2020.12.033

Abstract

Abstract: In order to understand the drug resistance of Riemerella anatipestifer from Muscovy duck,this study carried out bacterial isolation and culture,Gram staining microscopy,pathogen detection,biochemical test,16S rRNA sequence analysis,PCR identification,drug sensitivity test and drug resistance gene detection for Muscovy duck suspected of Riemerella anatipestifer infection.The results of bacterial isolation showed that on the blood agar medium,the isolated bacteria grew creamy needle tip size colonies with smooth surface,neat edge,luster and translucency.The Gram-negative bacillus brevis was detected by Gram-negative staining microscopy,and it was named GZQN201907.In the biochemical test of GZQN201907,urea reaction was positive,but glucose,maltose,lactose and other biochemical reactions were negative.The 16S rRNA phylogenetic tree was in the same branch as Riemerella anatipestifer.And the OmpA gene of PCR identification results of the isolated strain were positive.The drug sensitivity test was sensitive to 18 antibiotics including cefuroxime,erythromycin and ceftazidime,moderately sensitive to carboxypicillin and ciprofloxacin,and sensitive to neomycin and cotrimoxazole.And the resistance genes could detect the β-lactam resistance genes VIM and TEM,tetracycline resistance genes tetB,macrolide resistance genes ermB and ermF.The results of drug sensitivity test and drug resistance gene detection indicated that GZQN201907 showed the same resistance phenotype and gene detection results for β-lactam,tetracycline and macrolide.In the animal regression test,all the ducklings inoculated with GZQN201907 died within 72 h,while the control group showed no symptoms,indicating that GZQN201907 was virulent to the ducklings.One strain of Riemerella anatipestifer from Muscovy duck was successfully isolated,which laid a foundation for the prevention and treatment of Riemerella anatipestifer from muscovich.

Key words: Muscovy duck; Riemerella anatipestifer; drug resistance gene

CLC Number:

S855.1⁺2

XIAO Kun, CHEN Guang, ZHANG Jun, WEN Guilan, WEN Ming, CHENG Zhentao. Isolation,Identification and Drug Resistance Analysis of Riemerella anatipestifer from Muscovy Duck[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 4085-4092.

References

[1] 刁有祥.禽病学[M].北京:中国农业科学技术出版社,2012. DIAO Y X.Avian Disease[M].Beijing:China Agricultural Science and Technology Press,2012.(in Chinese)
[2] 查湖生,聂睿,孙志辉,等.雏番鸭鸭疫里默氏杆菌病的病原鉴定与耐药性分析[J].中国家禽,2014,36(3):49-51. ZHA H S,NIE R,SUN Z H,et al.Pathogen identification and drug resistance analysis of Riemerella anatipestifer disease[J].China Poultry,2014,36(3):49-51.(in Chinese)
[3] 刘闯,陈锋,廖理克,等.鸭疫里默氏杆菌血清型的研究进展[J].湖北农业科学,2008,3:355-357. LIU C,CHEN F,LIAO L K,et al.Research progress on serotype of Riemerella anatipestifer[J].Hubei Agricultural Science,2008,3:355-357.(in Chinese)
[4] 焦哲,宋彦民,孙秀秀,等.鸭疫里默氏杆菌与鸭源呼肠孤病毒混合感染的病理学诊断[J].中国兽医科学,2019,49(9):1194-1198. JIAO Z,SONG Y M,SUN X X,et al.Pathological diagnosis of mixed infection of Riemerella anatipestis with duck reovirus[J].Chinese Veterinary Science,2019,49(9):1194-1198.(in Chinese)
[5] 李迎晓.鸭疫里默氏杆菌病和棘口吸虫病混合感染诊治[J].黑龙江畜牧兽医,2016,22:138-139. LI Y X.Diagnosis and treatment of mixed infection of Riemerella anatipestifer and paragonimiasis[J].Heilongjiang Animal Science and Veterinary Medicine,2016,22:138-139.(in Chinese)
[6] 陈翠腾,程龙飞,傅光华,等.一例鸭1型甲肝病毒和11型鸭疫里默氏杆菌混合感染的诊断及其病原的分离鉴定[J].中国畜牧兽医,2016,43(11):3053-3058. CHEN C T,CHENG L F,FU G H,et al.Diagnosis of duck hepatitis virus type 1 and serotype 11Riemerella anatipestifer mixed infection and isolation and identification of pathogens[J].China Animal Husbandry & Veterinary Medicine,2016,43(11):3053-3058.(in Chinese)
[7] 段志强,嵇辛勤,赵佳福,等.新城疫病毒和鸭疫里默氏杆菌混合感染三穗鸭的诊治[J].中国家禽,2015,37(17):57-59. DUAN Z Q,JI X Q,ZHAO J F,et al.Diagnosis and treatment of mixed infection of Newcastle disease virus and Riemerella anatipestifer in Sansui ducks[J].China Poultry,2015,37(17):57-59.(in Chinese)
[8] 包细明.鸭主要病原菌耐药性分析及噬菌体的分离鉴定[D].贵阳:贵州大学,2019. BAO X M.Analysis of drug resistance of duck main pathogens and isolation and identification of phage[D].Guiyang:Guizhou University,2019.(in Chinese)
[9] 孙淑红,诸葛宝忠,朱德全,等.多重耐药黏液型铜绿假单胞菌氨基糖苷类修饰酶基因检测[J].中国微生态学杂志,2019,31(11):1269-1273. SUN S H,ZHUGE B Z,ZHU D Q,et al.Detection of aminoglycoside modifying enzyme genes in multi-drug resistant mucoid Pseudomonas aeruginosa[J].Chinese Journal of Microecology,2019,31(11):1269-1273.(in Chinese)
[10] 陈伟.动物源大肠杆菌耐药基因与HPI毒力基因的检测及相关性分析[D].合肥:安徽农业大学,2013. CHEN W.Detection and correlation analysis of drug resistance gene and HPI virulence gene of E.coli from animals[D].Hefei:Anhui Agricultural University,2013.(in Chinese)
[11] 邱军,张子威,樊瑞锋,等.呼伦贝尔地区猪源大肠杆菌血清型鉴定与耐药基因检测[J].东北农业大学学报,2015,46(7):50-56. QIU J,ZHANG Z W,FAN R F,et al.Serotype identification and resistance gene detection of swine Escherichia coli in Hulunbeir[J].Journal of Northeast Agricultural University,2015,46(7):50-56.(in Chinese)
[12] 张立平,张忠,曾瑾,等.宁夏地区162株大肠杆菌临床分离株对四环素类药物的耐药性分析[J].广东医学,2013,34(23):3580-3583. ZHANG L P,ZHANG Z,ZENG J,et al.Analysis of resistance of 162 clinical isolates of Escherichia coli to tetracycline drugs in Ningxia[J].Guangdong Medical Journal,2013,34(23):3580-3583.(in Chinese)
[13] 田国明,张敬峰.1例番鸭H9亚型禽流感病毒与鸭疫里氏杆菌混合感染的诊治[J].畜牧与兽医,2014,46(9):127. TIAN G M,ZHANG J F.Diagnosis and treatment of mixed infection of Muscovy duck H9 subtype avian influenza virus and Riebsiella anatipestifer[J].Animal Husbandry and Veterinary Medicine,2014,46(9):127.(in Chinese)
[14] 袁火升,李太国,王胜全,等.番鸭大肠杆菌和鸭疫里默氏菌混合感染的治疗[J].湖北畜牧兽医,2015,36(6):46-47. YUAN H S,LI T G,WANG S Q,et al.Treatment of mixed infection of E.coli and Riemerella anatipestifer[J].Hubei Journal of Animal and Veterinary Sciences,2015,36(6):46-47.(in Chinese)
[15] HU Q,HAN X,ZHOU X,et al.OmpA is a virulence factor of Riemerella anatipestifer[J].Veterinary Microbiology,2011,150(3-4):278-283.
[16] LI X Z,PLÉSIAT P,NIKAIDO H.The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria[J].Clinical Microbiology Reviews,2015,28(2):337-418.
[17] ZHANG X,WANG M S,LIU M F,et al.Contribution of RaeB,a putative RND-type transporter to aminoglycoside and detergent resistance in Riemerella anatipestifer[J].Frontiers in Microbiology,2017,8(1):2435.
[18] CHEN Q,GONG X,ZHENG F,et al.Interplay between the phenotype and genotype,and efflux pumps in drug-resistant strains of Riemerella anatipestifer[J].Frontiers in Microbiology,2018,9(1):2136.
[19] GYURIS É,WEHMANN E,CZEIBERT K,et al.Antimicrobial susceptibility of Riemerella anatipestifer strains isolated from geese and ducks in Hungary[J].Acta Veterinaria Hungarica,2017,65(2):153-165.
[20] LI T,SHAN M,HE J,et al.Riemerella anatipestifer M949_0459 gene is responsible for the bacterial resistance to tigecycline[J].Oncotarget,2017,8(57):96615-96626.

Isolation,Identification and Drug Resistance Analysis of Riemerella anatipestifer from Muscovy Duck

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