发酵脐橙粕对番鸭肠道形态结构、肠黏膜免疫功能和微生物多样性的影响

doi:10.16431/j.cnki.1671-7236.2023.02.015

Abstract

Abstract: 【Objective】 The feasibility of fermented navel orange pomace for Muscovy duck breeding was explored by investigating the effects of fermented navel orange pomace on intestinal morphology,intestinal mucosal immune function and microbial diversity in Muscovy ducks.【Method】 A total of 180 Muscovy ducks (16-day-old) weighing (267.33±9.50) g were randomly divided into two groups (half male and half female in each group), 6 replicates each group,15 ducks each repetition.The control group was fed the basal diet,and the experimental group was supplemented with 100 g/kg fermented navel orange pomace in the basal diet.The prefeeding period was 7 days,and the formal experiment lasted for 48 days.At the end of the experimental period,the duodenal tissues and cecal contents were collected from Muscovy ducks.The duodenal tissue samples were fixed with 4% paraformaldehyde.The intestinal morphological indexes and intestinal mucosal immune function index were detected by HE staining and immunohistochemistry,respectively.High-throughput sequencing technology was used to sequence 16S rDNA of cecal contents from experimental Muscovy ducks to analyze the changes of cecal microbial diversity.【Results】 ①Compared to control group,100 g/kg fermented navel orange pomace could extremely significantly increased the villi height (P＜0.01),as significantly elevated surface area of villi and positive rate of intraepithelial lymphocyte (P＜0.05) in duodenum of Muscovy duck.②Differential analysis between two groups showed that 100 g/kg fermented navel orange pomace extremely significantly inhibited the abundance of harmful bacteria such as Deferribacterota,Anaerovoracaceae and Acholeplasma (P＜0.01),and extremely significantly increased the abundance of beneficial bacteria such as Flavonifractor, Helicobacter and Megamonas (P＜0.01) compared to control group.Results of function prediction showed that 100 g/kg fermented navel orange pomace clearly depressed metabolism of terpenoids and polyketides of KEGG pathways in microbes of the cecum(P＜0.05) compared to control group. 【Conclusion】 Fermented navel orange pomace could improve intestinal morphology and structure,enhance the mucosal immune function of intestine and regulate the intestinal microbial diversity and metabolism.

Key words: fermented navel orange pomace; Muscovy ducks; intestinal morphology; intestinal mucosal immune function; microbial diversity

CLC Number:

S834

LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.

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Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks

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