丁酸钠对肉鸡肠道形态与消化吸收功能影响的研究进展

doi:10.16431/j.cnki.1671-7236.2021.05.011

Abstract

Abstract: The intestine is an important digestive,endocrine and immune organ of broilers,and intestinal health depends on the dynamic balance between nutrients,microbial flora and intestinal mucosa.Intestinal digestion and absorption are directly related to the growth of intestinal epithelial cells and intestinal morphology integrity.As an energy source,sodium butyrate can stimulate intestinal epithelial cell proliferation,and improve intestinal mucosal morphology in broiler and promote villus growth and intestinal tissue development.Through the intestinal free fatty acid receptors FFAR2 and FFAR3,sodium butyrate mediates the secretion of various hormones from intestinal endocrine cells,promotes gastrointestinal mucosa development,and stimulates the secretion of digestive enzymes from the stomach and pancreas,and promote nutrient digestion and absorption.Sodium butyrate is an essential regulator of intestinal homeostasis,which can stimulate mucin production,increase the thickness of mucus layer,reduce the permeability of colonic epithelium,and maintain intestinal integrity and mucosal barrier function.Sodium butyrate promotes the synthesis of host defense peptides (HPDs),inhibits intestinal harmful bacteria's proliferation,and reduces endotoxin's damage to intestinal epithelial cells.By inhibiting NF-κB kinase (IKK),sodium butyrate can down-regulate the pro-inflammatory pathway,inhibit the activation of NF-κB,prevent intestinal mucosal inflammation,promote digestion and absorption,and protect intestinal health.Therefore,sodium butyrate has intestinal protection and antibacterial effect,enhances intestinal integrity,promote digestion and absorption of nutrients and improves immunity and disease resistance.Under the background of the prohibition of antibiotics in the feed industry,it is very important to study and apply sodium butyrate as an antibiotic substitute.The authors focused on the mechanism of the effect of sodium butyrate on intestinal function,in order to provide a scientific basis for the replacement of antibiotics in broiler feed.

Key words: sodium butyrate; broiler; intestinal morphology; digestion and absorption

CLC Number:

S816.7

LI Xiang, HE Jinhuan, PAN Chunmei, SUO Jianghua, LI Jing. Research Progress on Effect of Sodium Butyrate on Intestinal Morphology, Digestion and Absorption Function of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1603-1612.

References

[1] SVIHUS B.Function of the digestive system[J].Journal of Applied Poultry Research, 2014,23(2):306-314.
[2] 陈业,赵怀宝.丁酸钠在动物肠道功能方面的生物学意义[J].饲料研究,2018,41(2):30-33. CHEN Y,ZHAO H B.Biological significance of sodium butyrate in intestinal function of animals[J].Feed Research,2018,41(2):30-33.(in Chinese)
[3] 肖宇,林英庭.丁酸钠在养鸡生产中的研究进展[J].饲料博览,2011,24(1):33-35. XIAO Y,LIN Y T.Progress of sodium butyrate in chickens production[J].Feed Review,2011,24(1):33-35.(in Chinese)
[4] 左丽君,姜宁,张爱忠.丁酸钠对畜禽机体营养及免疫调控作用的研究进展[J].中国畜牧杂志,2019,55(4):25-28. ZUO L J,JIANG N,ZHANG A Z.Advances in nutrition and immune manipulation action of sodium butyrate on livestock and poultry[J].Chinese Journal of Animal Science,2019,55(4):25-28.(in Chinese)
[5] 陈国顺,徐振飞,赵芳芳,等.包被丁酸钠对断奶仔猪生长性能和肠道消化酶活性的影响[J].国外畜牧学(猪与禽),2011,31(2):65-68. CHEN G S,XU Z F,ZHAO F F,et al.Effects of coated sodium butyrate on growth performance and intestinal digestive enzyme activities of weaned piglets[J].Animal Science Abroad(Pigs and Poultry),2011,31(2):65-68.(in Chinese)
[6] 李海英,张俐华,翟卫爽,等.丁酸钠替代饲用抗生素对白羽肉仔鸡生产性能和免疫器官指数的影响[J].新疆农业科学,2014,51(4):755-760. LI H Y,ZHANG L H,ZHAI W S,et al.Effect of sodium butyrate used as substitute for antibiotics feed additive on production performance and immune organ index of White broilers[J].Xinjiang Agricultural Sciences,2014,51(4):755-760.(in Chinese)
[7] LIN F,LI X,WEN J,et al.Effects of coated sodium butyrate on performance,diarrhea,intestinal microflora and barrier function of pigs during the first 2-week post-weaning[J].Animal Feed Science and Technology,2020,263(5):114464.
[8] CONWAY P L.Function and regulation of the gastrointestinal microbiota of the pig[A].Proceedings of the 6th International Symposium on Digestive Physiology in Pigs[C].1994.
[9] 何昭阳,王增辉.雏鸡消化道主要正常菌群定植规律的研究[J].畜牧兽医学报,2000,31(1):41-48. HE Z Y,WANG Z H.Study on the colonization law of main normal flora in the digestive tract of chicks[J].Journal of Animal Husbandry and Veterinary Medicine, 2000,31(1):41-48.(in Chinese)
[10] SCANES C G.Sturkie's Avian Physiology[M].London:Academic Press,2015.
[11] 冯焱,张芬鹊,李建慧.家禽肠道黏膜屏障结构及功能研究进展[J].中国家禽,2016,38(4):1-4. FENG Y,ZHANG F Q,LI J H.Research progress on the structure and function of poultry intestinal mucosal barrier[J].China Poultry, 2016,38(4):1-4.(in Chinese)
[12] SAIF Y M.Diseases of Poultry[M].Iowa:Iowa State University Press,2003.
[13] APAJALAHTI J,KETTUNEN A,GRAHAM H.Characteristics of the gastrointestinal microbial communities,with special reference to the chicken[J].World's Poultry Science Journal,2004,60(2):223-232.
[14] FREDERICK W.Advances in Immunology[M].London:Academic Press,2014.
[15] VINOLO M A,RODRIGUES H G,Nachbar R T,et al.Regulation of inflammation by short chain fatty acids[J].Nutrients, 2011,3(10):858-876.
[16] TIMBERMONT L,LANCKRIET A,DEWULF J,et al.Control of Clostridium perfringens-induced necrotic enteritis in broilers by target-released butyric acid,fatty acids and essential oils[J].Avian Pathology,2010,39(2):117-121.
[17] LIU J,LUMPKINS B,MATHIS G,et al.Evaluation of encapsulated sodium butyrate with varying releasing times on growth performance and necrotic enteritis mitigation in broilers[J].Poultry Science,2019,98(8):3240-3245.
[18] KINOSHITA M,SUZUKI Y,SAITO Y.Butyrate reduces colonic paracellular permeability by enhancing PPARγ activation[J].Biochemical and Biophysical Research Communications,2002,293(2):827-831.
[19] WU W,XIAO Z B,AN W Y,et al.Dietary sodium butyrate improves intestinal development and function by modulating the microbial community in broilers[J].PLoS One,2018,13(5):e0197762.
[20] 王继凤,李芙燕,陈耀星,等.丁酸钠对肉鸡小肠黏膜免疫相关细胞的影响[J].动物营养学报,2008,20(4):469-474. WANG J F,LI F Y,CHEN Y X,et al.Effects of sodium butyrate on mucosal immunity-associated cells in small intestine of broiler[J].Chinese Journal of Animal Nutrition, 2008,20(4):469-474.(in Chinese)
[21] 崔蕾,袁缨,权志中,等.不同水平的丁酸钠对肉仔鸡小肠肠道形态的影响[J].饲料工业,2009,30(5):34-37. CUI L,YUAN Y,QUAN Z Z,et al.Effects of different levels of sodium butyrate on intestinal morphology of broilers[J].Feed Industry,2009,30(5):34-37.(in Chinese)
[22] GONZÁLEZ-ORTIZ G,DOS SANTOS T,VIENOLA K,et al.Response of broiler chickens to xylanase and butyrate supplementation[J].Poultry Science, 2019,98(9):3914-3925.
[23] CHAMBA F,PUYALTO M,ORTIZ A,et al.Effect of partially protected sodium butyrate on performance,digestive organs,intestinal villi and E.coli development in broilers chickens[J].International Journal of Poultry Science,2014,13(7):390.
[24] AGHAZADEH A,TAHAYAZDI M.Effect of butyric acid supplementation and whole wheat inclusion on the performance and carcass traits of broilers[J].South African Journal of Animal Science,2012,42(3):241-248.
[25] MAHDAVI R,TORKI M.Study on usage period of dietary protected butyric acid on performance[J].Journal of Animal and Veterinary Advances,2009,8(9):1702-1709.
[26] ADIL S,MAGRAY S.Impact and manipulation of gut microflora in poultry:A review[J].Journal of Animal and Veterinary Advances,2012,11(6):873-877.
[27] LAN R,LI S,CHANG Q,et al.Sodium butyrate enhances growth performance and intestinal development in broilers[J].Czech Journal of Animal Science,2020,65(1):1-12.
[28] HU Z,GUO Y.Effects of dietary sodium butyrate supplementation on the intestinal morphological structure,absorptive function and gut flora in chickens[J].Animal Feed Science and Technology, 2007,132(3-4):240-249.
[29] MALLO J,PUYALTO M,RAO S R.Evaluation of the effect of sodium butyrate addition to broiler diets on energy and protein digestibility,productive parameters and size of intestinal villi of animals[J].Feed Compounder,2012,32(1):30-33.
[30] 刘馨忆.包被丁酸钠对肉仔鸡生长性能、免疫功能及肠道组织形态的影响[J].饲料研究,2020,43(6):41-44. LIU X Y.Effect of sodium butyrate on growth performance,immune function and intestinal morphology of broilers[J].Feed Research,2020,43(6):41-44.(in Chinese)
[31] 鞠婷婷,郭孝烨,肖雪,等.饲粮添加丁酸钠对黄羽肉鸡生产性能、血清生化指标、消化功能和肠道形态的影响[J].中国家禽,2015,37(9):32-36. JU T T,GUO X Y,XIAO X,et al.Effects of sodium butyrate on growth performance,serum biochemical indicators,digestive function and intestinal morphology in broilers[J].China Poultry,2015,37(9):32-36.(in Chinese)
[32] 张俊.丁酸酯和丁酸钠对急性应激肉仔鸡小肠形态结构、二糖酶活性及能量状态的影响[D].武汉:武汉轻工大学,2013. ZHANG J.Effects of tributyrin and coated sodium butyrate on morphology,disaccharidase and energy status of small intestine in acute stressed broilers[D].Wuhan:Wuhan Polytechnic University,2013.(in Chinese)
[33] LEESON S,NAMKUNG H,ANTONGIOVANNI M,et al.Effect of butyric acid on the performance and carcass yield of broiler chickens[J].Poultry Science, 2005,84(9):1418-1422.
[34] ELNESR S,ROPY A,ABDEL-RAZIK A.Effect of dietary sodium butyrate supplementation on growth,blood biochemistry,haematology and histomorphometry of intestine and immune organs of Japanese quail[J].Animal,2019,13(6):1234-1244.
[35] 杨在宾,邹杨,杨维仁,等.微囊丁酸钠对肉仔鸡肠道消化酶活性的影响[J].饲料工业,2011,S2:27-29. YANG Z B,ZOU Y,YANG W R,et al.Effects of microencapsulated sodium butyrate on digestive enzyme activities in intestinal tract of broilers[J].Feed Industry,2011,S2:27-29.(in Chinese)
[36] 邹杨.丁酸钠对商品肉仔鸡的促生长机理和应用功效研究[D].泰安:山东农业大学,2010. ZOU Y.Effects of sodium butyrate on growth-promoting mechanism and application efficiency in broilers[D].Tai'an:Shandong Agricultural University,2010.(in Chinese)
[37] ICHIKAWA H,SHINEHA R,SATOMI S,et al.Gastric or rectal instillation of short-chain fatty acids stimulates epithelial cell proliferation of small and large intestine in rats[J].Digestive Diseases and Sciences,2002,47(5):1141-1146.
[38] 郭传珍,曹兵海.丁酸钠对肉鸡肠道pH值、微生物菌群和挥发性脂肪酸的影响研究[J].中国家禽,2009,31(21):14-17. GUO C Z,CAO B H.Effects of sodium butyrate on intestinal pH value and intestinal colonic microflora population and volatile fatty acid of broilers[J].China Poultry, 2009,31(21):14-17.(in Chinese)
[39] PANDA A,RAO S,RAJU M,et al.Effect of butyric acid on performance,gastrointestinal tract health and carcass characteristics in broiler chickens[J].Asian-Australasian Journal of Animal Sciences, 2009,22(7):1026-1031.
[40] CZERWINSKI J,HOJBERG O,SMULIKOWSKA S,et al.Effects of sodium butyrate and salinomycin upon intestinal microbiota,mucosal morphology and performance of broiler chickens[J].Archives of Animal Nutrition, 2012,66(2):102-116.
[41] 邹杨,杨在宾,杨维仁,等.不同剂型丁酸钠与抗生素对肉仔鸡生产性能、肠道pH及挥发性脂肪酸含量的影响[J].动物营养学报,2010,22(3):675-681. ZOU Y,YANG Z B,YANG W R,et al.Effects of different preparations of sodium butyrate and antibiotics on performance,intestinal pH and concentration of volatile fatty acids in broilers[J].Chinese Journal of Animal Nutrition, 2010,22(3):675-681.(in Chinese)
[42] MOQUET P C A,ONRUST L,VAN IMMERSEEL F,et al.Importance of release location on the mode of action of butyrate derivatives in the avian gastrointestinal tract[J].Worlds Poultry Science Journal,2016,72(1):61-80.
[43] 张锦涛,伊曼,李志嘉,等.丁酸盐在炎症反应中作用机制的研究进展[J].免疫学杂志,2015,31(12):95-98. ZHANG J T,YI M,LI Z J,et al.Research progress on the mechanism of butyrate in inflammatory reaction[J].Immunological Journal,2015,31(12):95-98.(in Chinese)
[44] PETER J,MICHAEL K.Nuclear factor-kappaB:A pivotal transcription factor in chronic inflammatory disease[J].New England Journal of Medicine,1997,336(15):1066-1071.
[45] KLASING K C,JOHNSTONE B J.Monokines in growth and development[J].Poultry Science,1991,70(8):1781-1789.
[46] MOEINIAN M,GHASEMI-NIRI S F,MOZAFFARI S,et al.Synergistic effect of probiotics,butyrate and L-carnitine in treatment of IBD[J].Journal of Medical Hypotheses and Ideas,2013,7(2):50-53.
[47] HAMER H M,JONKERS D M,BAST A,et al.Butyrate modulates oxidative stress in the colonic mucosa of healthy humans[J].Clinical Nutrition,2009,28(1):88-93.
[48] CANANI R B,DI COSTANZO M,LEONE L,et al.Potential beneficial effects of butyrate in intestinal and extraintestinal diseases[J].World Journal of Gastroenterology,2011,17(12):1519.
[49] ZHANG W H,JIANG Y,ZHU Q F,et al.Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens[J].British Poultry Science,2011,52(3):292-301.
[50] 邱洪仪.三丁酸甘油酯和包被丁酸钠对慢性应激肉鸡肠道形态结构、二糖酶活性及脂质代谢的影响[D].武汉:武汉轻工大学,2013. QIU H Y.The effect of tributyrin and sodium butyrate on intestinal morphological structure,mucosa disaccharidase activity and lipid metabolism in broilers challenged with lipopolysaccharide[D].Wuhan:Wuhan Polytechnic University,2013.(in Chinese)
[51] CAMPBELL Y,FANTACONE M L,GOMBART A F.Regulation of antimicrobial peptide gene expression by nutrients and by-products of microbial metabolism[J].European Journal of Nutrition,2012,51(8):899-907.
[52] HAMER H M,JONKERS D,VENEMA K,et al.The role of butyrate on colonic function[J].Alimentary Pharmacology and Therapeutics,2008,27(2):104-119.
[53] LEWIS K,LUTGENDORFF F,PHAN V,et al.Enhanced translocation of bacteria across metabolically stressed epithelia is reduced by butyrate[J].Inflamma-tory Bowel Diseases, 2010,16(7):1138-1148.
[54] ABDELQADER A,AL-FATAFTAH A R.Effect of dietary butyric acid on performance,intestinal morphology,microflora composition and intestinal recovery of heat-stressed broilers[J].Livestock Science,2016,183(1):78-83.
[55] MAKLED M,ABOUELEZZ K,GAD-ELKAREEM A,et al.Comparative influence of dietary probiotic,yoghurt,and sodium butyrate on growth performance,intestinal microbiota,blood hematology,and immune response of meat-type chickens[J].Tropical Animal Health and Production,2019,51(8):2333-2342.
[56] FERNÁNDEZ-RUBIO C,ORDONEZ C,ABAD-GONZÁLEZ J,et al.Butyric acid-based feed additives help protect broiler chickens from Salmonella Enteritidis infection[J].Poultry Science,2009,88(5):943-948.
[57] VAN IMMERSEEL F,BOYEN F,GANTOIS I,et al.Supplementation of coated butyric acid in the feed reduces colonization and shedding of Salmonella in poultry[J].Poultry Science,2005,84(12):1851-1856.
[58] NAMKUNG H,YU H,GONG J,et al.Antimicrobial activity of butyrate glycerides toward Salmonella Typhimurium and Clostridium perfringens[J].Poultry Science,2011,90(10):2217-2222.
[59] KULKARNI R,PARREIRA V,SHARIF S,et al.Immunization of broiler chickens against Clostridium perfringens-induced necrotic enteritis[J].Clinical and Vaccine Immunology, 2007,14(9):1070-1077.
[60] GANTOIS I,DUCATELLE R,PASMANS F,et al.Butyrate specifically down-regulates Salmonella pathogenicity island 1 gene expression[J].Applied and Environmental Microbiology,2006,72(1):946-949.
[61] SUNKARA L T,ACHANTA M,SCHREIBER N B,et al.Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression[J].PLoS One,2011,6(11):e27225.
[62] GANZ T.Defensins:Antimicrobial peptides of innate immunity[J].Nature Reviews Immunology,2003,3(9):710-720.
[63] LANGHOUT D,SCHUTTE J,VAN LEEUWEN P,et al.Effect of dietary high-and low-methylated citrus pectin on the activity of the ileal microflora and morphology of the small intestinal wall of broiler chicks[J].British Poultry Science,1999,40(3):340-347.
[64] ANTONGIOVANNI M,BUCCIONI A,PETACCHI F,et al.Butyric acid glycerides in the diet of broiler chickens:Effects on gut histology and carcass composition[J].Italian Journal of Animal Science,2007,6(1):19-25.
[65] LATORRE R,STERNINI C,DE GIORGIO R,et al.Enteroendocrine cells:A review of their role in brain-gut communication[J].Neurogastroenterology & Motility,2016,28(5):620-630.
[66] REHFELD J.A centenary of gastrointestinal endocrinology[J].Hormone and Metabolic Research,2004,36(11/12):735-741.
[67] GUNAWARDENE A R,CORFE B M,STATON C A.Classification and functions of enteroendocrine cells of the lower gastrointestinal tract[J].International Journal of Experimental Pathology,2011,92(4):219-231.
[68] EGEROD K L,ENGELSTOFT M S,GRUNDDAL K V,et al.A major lineage of enteroendocrine cells coexpress CCK,secretin,GIP,GLP-1,PYY,and neurotensin but not somatostatin[J].Endocrinology,2012,153(12):5782-5795.
[69] 高侃,慕春龙,余凯凡,等.肠道内分泌与营养素感应系统[J].动物营养学报,2016,28(6):1633-1640. GAO K,MU C L,YU K F,et al.Enteroendocrine and nutrient sensing system[J].Chinese Journal of Animal Nutrition,2016,28(6):1633-1640.(in Chinese)
[70] RAZA M,BISWAS A,MIR N,et al.Butyric acid as a promising alternative to antibiotic growth promoters in broiler chicken production[J].The Journal of Agricultural Science,2019,157(1):55-62.
[71] DEEPA K,PURUSHOTHAMAN M,VASANTHA-KUMAR P,et al.Effect of sodium butyrate as an antibiotic substitute on production performance,carcass characteristics and economics in broiler chicken[J].Animal Nutrition and Feed Technology,2018,18(3):377-387.

Research Progress on Effect of Sodium Butyrate on Intestinal Morphology, Digestion and Absorption Function of Broilers

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