宏基因组法研究不同堆肥处理下猪粪微生物动态结构特征

doi:10.16431/j.cnki.1671-7236.2021.07.044

Abstract

Abstract: To study the characteristic and composition of swine manure of two treatments during composting using metagenomic technology.Fecal samples were collected from the same pig tank and randomly divided into two treatments after stirring,such as F group which was added to EM bacteria and K group without EM bacteria.Each treatment was made into a conical pile with a height of 1.2 m and a diameter of 2 m at the bottom.The samples were collected at 0,7,14 and 21 days during composting of two treatments and used to extract genomic DNA.Fecal microbial diversity was investigated by metagenomic sequencing technology.The results showed that in total 1 083 607 ORFs were obtained after sequencing,and the bacteria in the swine manure included 147 phyla,118 classes,258 orders,593 families,2 343 genera,and 13 193 species.At phyla level,the relative abundance of the top 10 bacterias include that Proteobacteria,Bacteroidetes,Actinobacteria,Firmicutes,Verrucomicrobia,Chloroflexi,Tenericutes,Planctomycetes,Spirochaetes,and Acidobacteria.The top 35 bacterias of the two treatments at genera level with the relative abundance were classified into 5 phylas,respectively belong to Proteobacteria,Bacteroidetes,Firmicutes,Actinobacteria and Verrucomicrobia.The KEGG functional prediction showed that an abundance of functional genes such as those involved in amino acid transport and carbohydrate metabolism pathway.The differences in the structure of two groups showed that the addition of EM bacteria would promote the changes of the flora at the early stage of composting.

Key words: swine; manure; metagenomics sequencing; species composition; microbial diversity

CLC Number:

S851.2⁺4

PAN Meng, YE Jianqiang, KANG Runmin, XIAO Lu, YE Yonggang, YU Jifeng, XIE Jing, CAO Ye, LI Xingyu, LIN Yi, WEI Yong. Study on the Characteristics of Microbial Diversity in Swine Manure of Different Treatments During Fermentation by Metagemomics Sequencing[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2671-2684.

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Study on the Characteristics of Microbial Diversity in Swine Manure of Different Treatments During Fermentation by Metagemomics Sequencing

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