哺乳期幼驼与成年母驼粪便菌群多样性分析

doi:10.16431/j.cnki.1671-7236.2023.01.010

Abstract

Abstract: 【Objective】 The purpose of this experiment was to explore the structural composition and diversity of fecal flora in young suckling and adult female camels, and provide an important basis for healthy development of young camels.【Method】 12 adult female camels with 6-7 years old and similar physical condition, and 12 young suckling female camels with similar birth date (3 months old) were selected.The fresh fecal samples were collected by rectal fecal sampling method for V3-V4 region sequencing of 16S rDNA of fecal microbiota, and the sequencing data were analyzed accordingly.【Result】 The Chao1 and Shannon indexes of fecal flora in adult female camels were significantly higher than that of young suckling camels (P<0.05).At phylum levels, the top 10 bacteria in feces of adult female and young suckling camels were Firmicutes, Bacteroidetes, Verrucomicrobia, Proteobacteria, Tenericutes, Spirochaetes, Saccharibacteria, Actinobacteria, Cyanobacteria and Fibrobacteres.But the abundance of Proteobacteria and Tenericutes in feces of young suckling camels were extremely significantly higher than that of adult female camels (P<0.01).At family levels, the top 10 bacteria in feces of adult female and young suckling camels were Ruminococcaceae, Peptostreptococcaceae, Lachnospiraceae, Clostridiaceae, Christensenellaceae, Rikenellaceae, Verrucomicrobiaceae, Bacteroidaceae, Mogibacteriaceae and Paraprevotellaceae, and the abundance of Peptostreptococcaceae and Clostridiaceae in feces of adult female camels were extremely significantly higher than that of young suckling camels (P<0.01), while the abundance of Lachnospiraceae in feces of young suckling camels was extremely significantly higher than that of adult female camels (P<0.01).At genus levels, the top 10 bacteria in feces of adult female and young suckling camels were Clostridium, Oscillospira, Ruminococcus, Clostridiaceae_Clostridium, Akkermansia, 5-7 N15, CF231, Turicibacter, Clostridiaceae-SMB53 and Treponema, and the abundance of Clostridium, Clostridiaceae_Clostridium and Turicibacter in feces of adult female camels were extremely significantly higher than that of young suckling camels (P<0.01).【Conclusion】 The fecal flora diversity of adult female camels was significantly higher than that of young suckling camels, and show more stability.The dominant species of bacteria in feces of adult female and young suckling camels were consistent at the levels of phylum, family and genus, but there were difference in abundance.

Key words: young suckling camels; female adult camels; feces; microbial diversity

CLC Number:

S824.5

WANG Jiahao, WANG Jing, YUAN Peng, LI Zhiwei, GENG Juan, WANG Hui, LI Fengming, ZANG Changjiang. Diversity Analysis of Fecal Flora in Young Suckling Camels and Adult Female Camels[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 97-105.

References

[1] 李晓斌, 李海, 李凤鸣, 等.断奶前纯血马马驹粪便菌群多样性分析[J].动物营养学报, 2019, 31(9):4363-4370. LI X B, LI H, LI F M, et al.Analysis of fecal microflora diversity for thoroughbred foals before weaning[J].Chinese Journal of Animal Nutrition, 2019, 31(9):4363-4370.(in Chinese)
[2] SAMSUDIN A A, EVANS P N, WRIGHT A G, et al.Molecular diversity of the foregut bacteria community in the dromedary camel (Camelus dromedarius)[J].Environmental Microbiology, 2011, 13(11):3024-3035.
[3] MING L, YI L, SIRIGULENG, et al.Comparative analysis of fecal microbial communities in cattle and Bactrian camels[J].PLoS One, 2017, 12(3):e0173062.
[4] 李晓斌, 李海, 臧长江, 等.断奶前马驹与母马粪便菌群多样性分析[J].中国畜牧兽医, 2020, 47(4):1050-1057. LI X B, LI H, ZANG C J, et al.Diversity analysis of fecal floraon pre-weaning foal and mare[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(4):1050-1057.(in Chinese)
[5] SHENGYONG M, MENGLING Z, JUNHUA L, et al.Characterising the bacterial microbiota across the gastrointestinal tracts of dairy cattle:Membership and potential function[J].Scientific Reports, 2015, 5(1):16116.
[6] BIBO L, KE Z, CHAO L, et al.Characterization and comparison of microbiota in the gastrointestinal tracts of the goat (Capra hircus) during preweaning development[J].Frontiers in Microbiology, 2019, 10:2125.
[7] WENJING Z, YAPENG W, SHUYUN L, et al.The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments[J].PLoS One, 2015, 10(2):e0117441.
[8] 张瑞阳, 孟玲, 李方方, 等.包被丁酸钠对断奶仔猪生长性能、血清生化指标、养分表观消化率和粪便微生物菌群的影响[J].动物营养学报, 2019, 31(5):2296-2302. ZHANG R Y, MENG L, LI F F, et al.Effects of coated sodium butyrate on growth performance, serum biochemical indices, nutrient apparent digestibility and fecal microflora population of weaning piglets[J].Chinese Journal of Animal Nutrition, 2019, 31(5):2296-2302.(in Chinese)
[9] 马露, 刘文慧, 阿拉腾珠拉, 等.外源添加短链脂肪酸调控断奶前犊牛胃肠道健康发育潜在功能和机制[J].动物营养学报, 2019, 31(6):2465-2470. MA L, LIU W H, ALATENGZHULA, et al.Potential functions and mechanisms of exogenous addition short chain fatty acids regulated gastrointestinal tract healthy development of calves before weaning[J].Chinese Journal of Animal Nutrition, 2019, 31(6):2465-2470.(in Chinese)
[10] 李婷.健康与腹泻羔羊生长发育及肠道菌群变化的相关性研究[D].乌鲁木齐:新疆农业大学, 2018. LI T.Relationship between lamb growth and gastrointestinal flora changes in health and diarrhea[D].Urumqi:Xinjiang Agricultural University, 2018.(in Chinese)
[11] 王瑶, 李卓, 杨超, 等.粪便微生物系移植通过影响炎症因子和肠道菌群结构缓解大鼠实验性结肠炎[J].中国生物化学与分子生物学报, 2017, 33(9):917-924. WANG Y, LI Z, YANG C, et al.Fecal microbiota transplantation ameliorates colorectal colitis in rats by affecting inflammatory cytokines and gut microbiota[J].Chinese Journal of Biochemistry and Molecular Biology, 2017, 33(9):917-924.(in Chinese)
[12] 向全航.菌群发育窗口期早期干预对仔猪肠道菌群与肠道先天性免疫系统发育的影响及机制[D].武汉:华中农业大学, 2020. XIANG Q H.Effect and mechanism of intervention during window period on gut microbiota and intestinal innate immune development in piglets[D].Wuhan:Huazhong Agricultural University, 2020.(in Chinese)
[13] CHUNG H, PAMP S J, HILL J A, et al.Gut immune maturation depends on colonization with a host-specific microbiota[J].Cell, 2012, 149(7):1578-1593.
[14] 何静.双峰驼肠道微生态特征及纤维素分解菌的研究[D].呼和浩特:内蒙古农业大学, 2019. HE J.A study on the gut microbiome and cellulolytic bacierium in bactriancamels[D].Hohhot:Inner Mongolia Agricultural University, 2019.(in Chinese)
[15] VAIBHAV B, SUCHITRA D, NITIN P, et al.Molecular analysis of the bacterial microbiome in the forestomach fluid from the dromedary camel (Camelus dromedarius)[J].Molecular Biology Reports, 2013, 40(4):3363-3371.
[16] YUJIAN W, HAO Z, LIN Z, et al.Dynamic distribution of gut microbiota in goats at different ages and health states[J].Frontiers in Microbiology, 2018, 9:2509.
[17] DE MUINCK E J, TROSVIK P.Individuality and convergence of the infant gut microbiota during the first year of life[J].Nature Communications, 2018, 9(1):1-8.
[18] SHIN N, WHON T W, BAE J.Proteobacteria:Microbial signature of dysbiosis in gut microbiota[J].Trends in Biotechnology, 2015, 33(9):496-503.
[19] GITALEE S, KATHRIN L, RAHEL K, et al.Maternal overnutrition during critical developmental periods leads to different health adversities in the offspring:Relevance of obesity, addiction and schizophrenia[J].Scientific Reports, 2019, 9(1):17322.
[20] MELANIE S, ASHLEY G, HERA V, et al.Microbial genes and pathways in inflammatory bowel disease[J].Nature Reviews.Microbiology, 2019, 17(8):497-511.
[21] DE OLIVEIRA M N V, JEWELL K A, FREITAS F S, et al.Characterizing the microbiota across the gastrointestinal tract of a Brazilian Nelore steer[J].Veterinary Microbiology, 2013, 164(3-4):307-314.
[22] MAXIME B, JULIEN T, ARIANE L, et al.Faecalmicrobiota study reveals specific dysbiosis in spondyloarthritis[J].Annals of the Rheumatic Diseases, 2017, 76(9):1614-1622.
[23] DOUA A, AIDANA O, ADRIANA H, et al.Lupus nephritis is linked to disease-activity associated expansions and immunity to a gut commensal[J].Annals of The Rheumatic Diseases, 2019, 78(7):947-956.
[24] JILLIAN W, RUTH L.The human gut bacteria christensenellaceae are widespread, heritable, and associated with health[J].BMC Biology, 2019, 17(1):83.
[25] KONG F, HUA Y, ZENG B, et al.Gut microbiota signatures of longevity[J].Current Biology, 2016, 26(18):R832-R833.
[26] MORETÓ M, MIRÓ L, AMAT C, et al.Dietary supplementation with spray-dried porcine plasma has prebiotic effects on gut microbiota in mice[J].Scientific Reports, 2020, 10(1):2926.
[27] FABRICE A, MIREILLE H, BERNARD V, et al.Monitoring bacterial community of human gut microbiota reveals an increase in lactobacillus in obese patients and methanogens in anorexic patients[J].PLoS One, 2009, 4(9):e7125.
[28] YUYING D, ZHENXING H, MINGXING Z, et al.Effects of co-inoculating rice straw with ruminal microbiota and anaerobic sludge:Digestion performance and spatial distribution of microbial communities[J].Applied Microbiology and Biotechnology, 2017, 101(14):5937-5948.
[29] JENNIFER B, DIONYSIOS A, BERG M M E, et al.Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases[J].Proceedings of The National Academy of Sciences of the United States of America, 2009, 106(6):1948-1953.
[30] PEARL H, ALLAN W W.Why is initial bacterial colonization of the intestine important to infants' and children's health?[J].Journal of Pediatric Gastroenterology and Nutrition, 2015, 60(3):294-307.
[31] HASSAN Z, H S M.Gut bacteroides species in health and disease[J].Gut Microbes, 2021, 13(1):21-20.
[32] SUKHPREET T, HEIN T, THEODORE K, et al.Bacteroides-dominant gut microbiome of late infancy is associated with enhanced neurodevelopment[J].Gut Microbes, 2021, 13(1):11-17.

Diversity Analysis of Fecal Flora in Young Suckling Camels and Adult Female Camels

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments

[1]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[2]	TIAN Quanhua, ZHAO Xu, LIU Yong, WEI Lingyun, TAN Zhiliang, HE Zhixiong. Effects of Astragalus membranaceus and Dextran Sodium Sulfate Stimulation on Intestinal Hindgut Fermentation and Microbial Composition of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3242-3253.
[3]	PAN Meng, YE Jianqiang, KANG Runmin, XIAO Lu, YE Yonggang, YU Jifeng, XIE Jing, CAO Ye, LI Xingyu, LIN Yi, WEI Yong. Study on the Characteristics of Microbial Diversity in Swine Manure of Different Treatments During Fermentation by Metagemomics Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2671-2684.
[4]	LI Wenjing, WEI Kaimin, ZHANG Shaoqiu, CHEN Yong. Effects of Walnut Green Husk and Its Extract on Intestinal Morphology,Mucosal Antioxidant Activity and Microbial Diversity of Yellow-feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2056-2065.
[5]	ZHANG Huihui, LI Mengwei, TANG Zhenhua, LIANG Xin, PENG Lijuan, PENG Kaiping, YANG Chengjian, WANG Xinfeng. Effects of Cysteamine on the Lactation Performance,Antioxidant Performance and Rumen Microbial Diversity of Buffalo in Summer [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 901-915.
[6]	ZHANG Anrong, LIU Jiao, CHEN Zhimin, CHANG Wenhuan, DENG Xuejuan, CAI Huiyi, LIU Guohua, ZHENG Aijuan. Effects of Clostridium butyricum on Calcium and Phosphorus Metabolism and Tibia Indexes of Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2849-2856.
[7]	ZHANG Huihui, LI Mengwei, TANG Zhenhua, LIANG Xin, PENG Lijuan, PENG Kaiping, YANG Chengjian, WANG Xinfeng. Effects of Different Levels of Chromium Niacin on Lactation Performance, Antioxidant Activity and Ruminal Microbial Diversity in Buffalo Under Heat Stress [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1373-1383.
[8]	LIU Zhenni, LEI Xiaowen, KONG Zhiwei, ZHONG Yunping, XIE Hualiang, TAN Donghai, WU Yijian. Effects of Fermented Peanut Vine on Production Performance,Slaughter Performance, Serum Biochemical Indexes and Feces' Routine Ingredients in Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3493-3501.
[9]	CHEN Qianqian, LIU Bo, WANG Jieping, CHE Jianmei, ZHANG Haifeng, ZHU Yujing. Piggery Air Microbial Community of Microbial Fermentation Bed [J]. , 2018, 45(2): 536-543.
[10]	YANG Ya-jin, GUO Ai-wei, ZHANG Ying, SUN Ya-jie, HUANG Yan-nao. Effects of Lactobacillus plantarum on Production Performance,Egg and Feces Bacteria content of Salmonella enteric-Infected Hens [J]. , 2017, 44(2): 482-487.
[11]	KANG Run-min, LI Yao, LV Xue-bin, JI Gao-sheng, YING San-cheng, ZENG Kai, LI Yan, YIN Ming-yu. Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16S rDNA Tag [J]. , 2017, 44(11): 3121-3129.
[12]	WANG Shui-ping, WANG Wen-juan. Research Progress on the Factors Influencing the Amount of Methane Emission of Ruminants [J]. , 2013, 40(2): 225-227.
[13]	YU Miao;YIN Yong-zhi;GUO Chun-hua;AI Dan;CAO Long-kai;YAN Jin-xiu;YU Ting-ting;XIE Xiao-hong;GUO Zhi-qiang. Effect of Different Levels of Silkworm Feces on Meat Quality of New Zealand Rabbit [J]. , 2011, 38(7): 13-15.
[14]	WANG Jia-qi. Five Key Indicators Leading the Direction of China Dairy Industry [J]. , 2011, 38(2): 5-9.