微生物发酵床猪舍空气微生物种群结构的研究

doi:10.16431/j.cnki.1671-7236.2018.02.031

Abstract

Abstract:

To explore the characteristics of air microorganism of piggery with microbial fermentation bed, and provide theoretical basis for the assessment of air quality of microbial fermentation bed, air samples were collected by the method of natural sedimentation. Air microorganism were cultured and identified based on 16S rRNA sequence analysis for investigating the diversity of piggery air microorganism. More than 60 strains were isolated and sequenced. Ultimately, 27 species were selected for subsequent analysis. The results showed that, among these 27 species, there were 14 Bacillus (51.9%), 5 Pseudomonas (18.5%), 4 Staphylococcus (14.8%), 1 Ochrobactrum, 1 Paenibacillus, 1 Lysinibacillus and 1 Luteimonas. 5 species of opportunistic pathogen and 6 species of organic degradation bacteria were found among these airborne bacteria. The airborne microbes were significantly clustered into 6 categories by SPSS analysis:Bacteria only distrusted in downwind piggery, bacteria only distrusted in windward piggery, bacteria only distrusted in outside piggery, bacteria distrusted in both windward and downwind piggery, bacteria distrusted in both downwind and outside piggery, and bacteria distrusted in all these three places.The species of airborne bacteria in downwind piggery were more than those of outside piggery and windward piggery. There were 20 species in downwind piggery, including 15 specific species. The adjacent locations had similar microbial composition because the microorganisms transported in the form of aerosol. Microbial fermentation bed has rich diversity of air microbial taxa, and the distributions of air microbial are affected by the wind.

Key words: microbial fermentation bed; air microorganism; natural sedimentation method; microbial diversity

CLC Number:

S851.2⁺4

CHEN Qianqian, LIU Bo, WANG Jieping, CHE Jianmei, ZHANG Haifeng, ZHU Yujing. Piggery Air Microbial Community of Microbial Fermentation Bed[J]. , 2018, 45(2): 536-543.

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Piggery Air Microbial Community of Microbial Fermentation Bed

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