半胱胺对夏季水牛泌乳性能、抗氧化性能和瘤胃微生物多样性的影响

doi:10.16431/j.cnki.1671-7236.2021.03.014

Abstract

Abstract: The purpose of this experiment was to study the effect of adding cysteamine to the buffalo diet on the lactation performance,rumen fermentation parameters,antioxidant performance,and rumen microbial diversity of the buffalo.Twenty healthy Nili-Ravi buffaloes with similar body weight (615 kg±21 kg),parity (3~5 births) and milk production (6 kg/d±1 kg/d) were selected and divided into 4 groups with 5 buffalo in each group as a single-factor randomized block design.0 (control group),15,30 and 45 g/d cysteamine was added to the diet,respectively.The pre-feeding period was 1 week and the test period was 4 weeks.The results showed that:Compared with the control group,① Adding cysteamine had no significant effect on buffalo body surface temperature,rectal temperature and respiratory rate (P>0.05).② The addition of 30 and 45 g/d cysteamine in the diet significantly increased the DMI and the apparent digestibility of ADF (P<0.05).③ The addition of cysteamine in the diet could significantly increase the milk production of buffalo,4% milk fat corrected milk production,milk protein rate,total milk solids and the milk non-fat solids content (P<0.05).④ Dietary supplementation with 45 g/d cysteamine significantly increases the buffalo rumen MCP content (P<0.05).Dietary supplementation with cysteamine had no significant effect on the number of bacteria and fungi in rumen fluid of buffalo (P>0.05).Adding 15 g/d cysteamine significantly reduced the number of protozoa (P<0.05).When the dosage of cysteamine was 30 g/d,the number of rumen fluid protozoa was significantly increased (P<0.05).⑤ The addition of cysteamine in the diet significantly reduced the content of C14:1n5,C18:3n6 in milk and significantly increased the content of C18:1n9t,C19:0,C20:3n6 in milk (P<0.05).Adding 30 g/d cysteamine to the diet significantly reduced the content of C16:0,C18:0 and SFA in milk (P<0.05).Dietary supplementation of 30 and 45 g/d cysteamine significantly reduced the content of C20:4n6 (ARA) and UFA in milk (P<0.05).⑥ The buffalo serum T-AOC in cysteamine groups were significantly higher (P<0.05);MDA in the diet supplemented with 15 and 30 g/d cysteamine group was significantly lower than the control group (P<0.05);GSH-Px,total protein and globulin in the 30 and 45 g/d cysteamine group was significantly increased compared with the control group (P<0.05);Cor of the 15 and 45 g/d cysteamine group was significantly lower than that of the control group (P<0.05).The activity of AST in the 30 g/d cysteamine group was significantly increased compared with the control group (P<0.05).In conclusion,the addition of cysteamine in the diet could improve the lactation performance and antioxidant capacity of the buffalo in the mid-lactation period,and affect the rumen fermentation,milk fat fatty acid content and the number of rumen protozoan in the buffalo.Under the conditions of this experiment,the recommended amount of cysteamine added to the diet was 30 g/d.

Key words: cysteamine; buffalo; lactation performance; antioxidant performance; microbial diversity

CLC Number:

S816.7

ZHANG Huihui, LI Mengwei, TANG Zhenhua, LIANG Xin, PENG Lijuan, PENG Kaiping, YANG Chengjian, WANG Xinfeng. Effects of Cysteamine on the Lactation Performance,Antioxidant Performance and Rumen Microbial Diversity of Buffalo in Summer[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 901-915.

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Effects of Cysteamine on the Lactation Performance,Antioxidant Performance and Rumen Microbial Diversity of Buffalo in Summer

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[3]	LIU Zhenni, LEI Xiaowen, LI Jianjun, ZENG Qingyuan, TAN Donghai, ZHONG Yunping, ZHANG Qiang, XIE Hualiang. Effects of Fermented Navel Orange Pomace on Intestinal Morphology,Intestinal Mucosal Immune Function and Microbial Diversity of Muscovy Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 573-585.
[4]	WANG Jiahao, WANG Jing, YUAN Peng, LI Zhiwei, GENG Juan, WANG Hui, LI Fengming, ZANG Changjiang. Diversity Analysis of Fecal Flora in Young Suckling Camels and Adult Female Camels [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 97-105.
[5]	LIU Xinyong, TAN Zhengzhun, LI Hui, HUANG Jian, LUO Hua, ZENG Linghu, CHENG Juanru, ZHONG Huapei, YANG Chunyan, WANG Xiaoli, QIN Guangsheng. Effects of Heat Stress on Blood Biochemical Indexes of Murrah and Nili-Ravi Buffaloes [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 521-528.
[6]	XU Yuanyuan, MO Xia, HUANG Chenqian, FENG Chao, LU Xingrong, MA Xiaoya, YANG Chunyan, ZHENG Haiying, SHANG Jianghua. Cloning and Bioinformatics Analysis of TRAIL Gene in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3693-3703.
[7]	LIANG Shasha, PANG Chunying, DENG Tingxian, LU Xingrong, DUAN Anqin, MA Xiaoya, FANG Yanyan, LIANG Xianwei. Polymorphism of MFSD2A Gene and Its Association with Milk Production Traits in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3856-3865.
[8]	ZHENG Haiying, YANG Chunyan, LI Lingyu, TANG Liping, ZHENG Wei, SHANG Jianghua. Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 208-215.
[9]	LI Lingyu, ZHENG Haiying, CHEN Mingtang, TANG Liping, SHANG Jianghua, YANG Chunyan. Effects of Oocyte Origin on the Developmental Potential of Buffalo Somatic Cell Nuclear Transfer Embryos [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 241-247.
[10]	DUAN Anqin, LU Xingrong, MA Xiaoya, LIANG Shasha, DENG Tingxian. Effect of HSD17B4 Gene Interference on Milk Fat and Casein in Buffalo Mammary Epithelial Cell [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3128-3137.
[11]	MA Xiaoya, LIANG Yanyin, LU Xingrong, DUAN Anqin, LIANG Shasha, DENG Tingxian. Bioinformatics Analysis of ABCD Gene Family in Buffalo and Expression Analysis of ABCD1 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3171-3182.
[12]	PANG Chunying, LIANG Shasha, WEN Chongli, CHEN Mingtang, LU Chengwei, WEI Kelong, PAN Yuhong, LIANG Xianwei. Isolation and Identification of Plasma Exosomes in Buffalo Calves [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3200-3205.
[13]	TIAN Quanhua, ZHAO Xu, LIU Yong, WEI Lingyun, TAN Zhiliang, HE Zhixiong. Effects of Astragalus membranaceus and Dextran Sodium Sulfate Stimulation on Intestinal Hindgut Fermentation and Microbial Composition of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3242-3253.
[14]	PAN Meng, YE Jianqiang, KANG Runmin, XIAO Lu, YE Yonggang, YU Jifeng, XIE Jing, CAO Ye, LI Xingyu, LIN Yi, WEI Yong. Study on the Characteristics of Microbial Diversity in Swine Manure of Different Treatments During Fermentation by Metagemomics Sequencing [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2671-2684.
[15]	LI Wenjing, WEI Kaimin, ZHANG Shaoqiu, CHEN Yong. Effects of Walnut Green Husk and Its Extract on Intestinal Morphology,Mucosal Antioxidant Activity and Microbial Diversity of Yellow-feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2056-2065.