水牛ABCD基因家族生物信息学分析及ABCD1基因表达研究

doi:10.16431/j.cnki.1671-7236.2021.09.007

Abstract

Abstract: This study was aimed to indentify the members of ATP-binding cassette (ABC) transporter D (ABCD) gene and its expression in buffalo. The chromosome mapping, gene structure, conserved domains and physicochemical properties of ABCD gene family in buffalo were analyzed using bioinformatics. The expression of ABCD1 gene in buffalo tissues and mammary glands at different lactation stages was detected by Real-time quantitative PCR. The effects of 3 hormones on the expression of ABCD1 gene in mammary epithelial cells of buffalo were also detected. The results indicated that 9 ABCD gene were identified from the whole genome of buffalo, which included ABCD1, ABCD2, ABCD3 and ABCD4, and ABCD4 gene contained 6 different transcripts(ABCD4X1-ABCD4X6). ABCD1-ABCD4 were identified on chromosome X, 4, 6 and 11, respectively. ABCD gene included 9-22 introns, which coded 444-741 amino acid residues with the molecular weight ranging from 49.63-83.41 ku, and the isoelectric points ranging from 6.43-9.64.3 motifs were identified from ABCD protein, including ABC_membrane_2, Endonuclease_5 and ABC_tran. ABCD gene family could be divided into 4 subfamilies, and the evolutionary tree of different species showed that ABCD gene was highly conserved. Collinearity analysis results showed that there were 7 pairs of ABCD gene family with homologous lineages between buffalo and cattle. ABCD1 gene was expressed in 15 tissues of buffalo with a relatively high level in fat. The expression of ABCD1 gene was variable during lactation and exhibited a trend of low-high-low with the highest level at 50 days after parturition. The mammary gland cells in buffalo were treated with different concentrations of prolactin, estradiol and progesterone, the expression of ABCD1 gene was significantly higher than that of control group when the concentrations of prolactin, estradiol and progesterone were above 2.0, 0.3 and 4.5 μg/mL, respectively. The results showed that there were 9 genes in ABCD gene family of buffalo, among which ABCD1 gene was involved in the regulation of lactation. This study provided a reference for further exploring the function of ABCD family genes in mammary gland of buffalo.

Key words: buffalo; ABCD gene family; bioinformatics; expression; mammary epithelial cell

CLC Number:

MA Xiaoya, LIANG Yanyin, LU Xingrong, DUAN Anqin, LIANG Shasha, DENG Tingxian. Bioinformatics Analysis of ABCD Gene Family in Buffalo and Expression Analysis of ABCD1 Gene[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3171-3182.

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Bioinformatics Analysis of ABCD Gene Family in Buffalo and Expression Analysis of ABCD1 Gene

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