卵母细胞来源对水牛体细胞核移植胚胎发育潜能的影响

doi:10.16431/j.cnki.1671-7236.2022.01.026

Abstract

Abstract: [Objective] This study was to explore the effects of different oocyte's origin on developmental ability of reconstructed embryos after somatic cell nuclear transfer (SCNT) and the expression levels of key development related proteins. [Method] The experiment was divided into two groups:Ovum pick up (OPU) and slaughterhouse (SLH) groups. In the OPU group, the follicles of 10 non-lactating buffalo were punctured and aspirated by ultrasonic in vivo to collect oocytes. In SLH group, follicles were drawn from the ovaries of slaughterhouses to collect oocytes. All the COCs were matured in vitro for 22-24 h, respectively. The cumulus cells were removed by blowing, the oocytes with the first polar body were selected and enuvleated for SCNT using ear fibroblasts as donor cells, and the fusion rate, cleavage rate and blastocyst rate of SCNT reconstructed embryos were recorded respectively. Furthermore, the protein expression levels of E-cadherin and transcription factor Sox2 in two SCNT reconstructed embryos were determinated by immunofluorescence. [Result] The maturation rate of oocytes and the blastocyst rate of reconstructed embryos in OPU group was significantly higher than that of SLH group (P<0.05), but there were no significant differences in fusion rate and blastocyst rate of two SCNT reconstructed embryos (P>0.05). According to the analysis of immunofluorescence images, E-cadherin protein was located on the cell membrane, Sox2 protein was distributed in the cell nuclear membrane and cytoplasm, and the expression level of E-cadherin and Sox2 protein in OPU group SCNT embryos was significantly higher than that of SLH-SCNT embryos (P<0.05). [Conlusion] Buffalo oocytes collected in vivo were more suitable for the construction of SCNT reconstructed embryos.

Key words: buffalo; oocyte; E-cadherin; transcription factor Sox2

CLC Number:

S813.7

LI Lingyu, ZHENG Haiying, CHEN Mingtang, TANG Liping, SHANG Jianghua, YANG Chunyan. Effects of Oocyte Origin on the Developmental Potential of Buffalo Somatic Cell Nuclear Transfer Embryos[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 241-247.

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Effects of Oocyte Origin on the Developmental Potential of Buffalo Somatic Cell Nuclear Transfer Embryos

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