白藜芦醇对体外培养的水牛卵丘细胞增殖活力及其相关基因mRNA表达的影响

doi:10.16431/j.cnki.1671-7236.2022.01.022

Abstract

Abstract: [Objective] The experiment was to study the effect of resveratrol(RES) on the proliferation activity, hormone secretion, cumulus expansion as well as apoptosis and antioxidase of buffalo cumulus cells cultured in vitro. [Method] Cumulus cells were cultured in medium supplemented with different concentrations (0(control group), 1, 10, 20, 30, 40, 50 and 60 μmol/L) of RES, cell proliferation activity was measured using CCK-8 kit. The best RES treatment concentration and time were selected for subsequent tests. The concentrations of estradiol (E₂)and progesterone (P₄)in the culture medium were measured by ELISA method, and the expression level of genes related to cumulus expansion, apoptosis and antioxidase were determined by Real-time quantitative PCR method. [Result] Compared with control group the proliferation activities of cumulus cells cultured in medium supplemented with 1, 10 and 20 μmol/L RES within 24-36 h were significantly promoted. The cell viability was the strongest after 36 h treatment with 10 μmol/L RES, so it was used for cell treatment in subsequent experiments. When cultured in vitro for 36 h, the cell proliferation ability and the secretion of E₂ and P₄ of 10 μmol/L RES group were significantly increased (P<0.05). The relative mRNA expression of PTX3, PTGS2, Bcl-2 and SOD1 genes of 10 μmol/L group was significantly or extremely significantly increased (P<0.05;P<0.01), and the expression of Bax, Caspase-3 and p21 genes was significantly or extremely significantly decreased (P<0.05;P<0.01), while there was no significant difference in the expression of HAS2 and CAT genes (P>0.05). [Conclusion] 10 μmol/L RES could increase the proliferation activity of cumulus cells, promote hormone secretion and the expansion of cumulus cells, and enhance the antioxidant capacity and reduce apoptosis of cumulus cells in buffalo.

Key words: resveratrol; buffalo; cumulus cells; proliferation activity; gene expression

CLC Number:

S823.8⁺3

ZHENG Haiying, YANG Chunyan, LI Lingyu, TANG Liping, ZHENG Wei, SHANG Jianghua. Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 208-215.

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Effects of Resveratrol on Proliferation of Cumulus Cells Cultured in vitro and mRNA Expression Levels of Related Genes in Buffalo

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