利用16S rDNA扩增子测序技术分析不同品种猪盲肠微生物菌落多样性

doi:10.16431/j.cnki.1671-7236.2017.11.002

Abstract

Abstract:

To study the diversity and composition of the bacterial community from cecum samples from Yacha pig, Qingyu pig and Wujin pig, the investigation was designed to reveal mechanism of roughage resistance. Illumina amplicon sequencing of 16S rDNA Tag was used to analyze the cecum microbial diversity of Yacha pig, Qingyu pig and Wujin pig at 90 kg liveweight. The results indicated that the core flora of the three breeds were Bacteroidetes and Firmicutes at the phylum level and Prevotella and Bacteroides at the genus level. Meanwhile bacteria associated with cellulose decomposition were found in all three breeds, in which the number of Fibrobacter and Clostridium in Yacha pig were significantly higher than that in Qingyu pig and Wujin pig (P<0.05),and the number of Prevotella in Yacha pig was significantly higher than that in Wujin pig (P<0.05). The number of Bacteroides in Qingyu pig was significantly higher than that in Yacha pig (P<0.05).The number of Spirochaetes in Qingyu pig was significantly higher than that in Wujin pig (P<0.05). It could conclud that the composition of the cecum bacteria community was similar in above pig breeds, but there were significant differences in distribution and quantity. According to analysis of microbial related to cellulose digestibility, Yacha pig was the strongest among the three breeds, followed by the Qingyu pig and Wujin pig.

Key words: Yacha pig; Qingyu pig; Wujin pig; cecum; microbial diversity; cellulose digest ability

CLC Number:

S811.6

KANG Run-min, LI Yao, LV Xue-bin, JI Gao-sheng, YING San-cheng, ZENG Kai, LI Yan, YIN Ming-yu. Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16S rDNA Tag[J]. , 2017, 44(11): 3121-3129.

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Analysis of the Cecum Microbial Diversity of Different Pig Breeds by Illumina Amplicon Sequencing of 16S rDNA Tag

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