鸭疫里默氏菌对鸭盲肠组织形态结构和TLR4信号通路相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2020.06.008

Abstract

Abstract: In order to investigate the possible pathogenic mechanism of Riemerella anatipestifer,the morphological structure was observed using microscope,the thickness of intestinal mucosa,the height of intestinal villi and the depth of crypt were measured and counted,and the expression of TLR4 signal pathway related genes in the cecum of ducks after infected with Riemerella anatipestifer was detected by Real-time quantitative PCR.Pathological observation results showed that the intestinal structure was damaged after Riemerella anatipestifer infection,which was manifested as intestinal villus shedding,congestion,bleeding and lymphocyte infiltration and lymphocyte proliferation.The statistical results showed that the thickness of intestinal mucosa in infection group (383.58 μm) was significantly lower than that in control group (643.39 μm) at 2 d (P<0.05).The height of intestinal villi in infection group (173.04 and 168.68 μm) were significantly lower than that in control group (355.79 and 276.54 μm) at 2 and 5 day (P<0.05),respectively.The ratio of height of intestinal villi to crypt depth in infection group (3.42) was significantly lower than that of control group (5.34) at 9 d (P<0.05).Real-time quantitative PCR results showed that the expression of TLR4 signal pathway related genes of TLR4,MD2,MyD88,TRAF6,NF-κB,IL-4 and IL-8 mRNA was up-regulated at 2 and 5 d after infection.These results suggested that the infection of Riemerella anatipestifer could lead to the physical damage and inflammatory lesions of the cecum,and TLR4 signal pathway was involved in the inflammatory response process.

Key words: Riemerella anatipestifer; cecum; morphological structure; TLR4 signal pathway

CLC Number:

Q786

TAO Zhiyun, ZHU Chunhong, SHI Zuhao, XU Wenjuan, ZHANG Shuangjie, SONG Weitao, LIU Hongxiang, LI Huifang. Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1702-1708.

References

[1] 张大丙,郭玉璞.鸭疫里默氏菌[J].中国动物保健,2001,7:20-22. ZHANG D B,GUO Y P.Riemerella anatipestifer[J].Zhongguo Dongwu Baojian,2001,7:20-22.(in Chinese)
[2] 杨晓辉,程龙飞,陈红梅,等.鸭疫里默氏菌分子生物学研究进展[J].中国畜牧兽医,2012,39(4):183-187. YANG X H,CHENG L F,CHEN H M,et al.The progress of molecular biology in Rimerella anatipestifer[J].China Animal Husbandry & Veternary Medicine,2012,39(4):183-187.(in Chinese)
[3] WANG Y,YIN X,ZHOU Z,et al.Cas9 regulated gene expression and pathogenicity in Riemerella anatipestifer[J].Microbial Pathogenesis,2019,136:103706.
[4] HUANG L,WANG M,MO T,et al.Role of LptD in resistance to glutaraldehyde and pathogenicity in Riemerella anatipestifer[J].Frontiers in Microbiology,2019,10:1443.
[5] YUAN H,HUANG L,WANG M.Role of the gldK gene in the virulence of Riemerella anatipestifer[J].Poultry Science,2019,98(6):2414-2421.
[6] 冯焱,张芬鹊,李建慧.家禽肠道粘膜屏障结构及功能研究进展[J].中国家禽,2016,38(4):1-4. FENG Y,ZHANG F Q,LI J H.Research progress in intestinal mucosal barrier structure and function of poultry[J].China Poultry,2016,38(4):1-4.(in Chinese)
[7] NAWAB A,AN L,WU J,et al.Chicken Toll-like receptors and their significance in immune response and disease resistance[J].Internal Reviews of Immunology,2019,38(6):284-306.
[8] HAN C,XU J,LIU C H,et al.Modulation of TLR2 and TLR4 in macrophages following Trichinella spiralis infection[J].Helminthologia,2018,55(3):195-203.
[9] JIA H,LI G,TIAN Y,et al.Cloning expression and bioinformatics analysis of the duck TLR4 gene[J].British Poultry Science,2012,53(2):190-197.
[10] ZHAO W,HUANG Z,CHEN Y,et al.Molecular cloning and functional analysis of the duck TLR4 gene[J].International Journal of Molecular Sciences,2013,14(9):18615-18628.
[11] ZHANG Y,CHEN Y,GU T,et al.Effects of Salmonella Enterica serovar Enteritidis infection on egg production and the immune response of the laying duck Anas platyrhynchos[J].Peer Journals,2019,7:e6359.
[12] 柳劲松.鸟类盲肠的功能[J].生物学通报,2005,3:13-15. LIU J S.Functions of avain function[J].Bulletin of Biology,2005,3:13-15.(in Chinese)
[13] 李韦,周璐丽,王定发,等.槟榔提取物对球虫感染鸡盲肠组织结构的影响[J].黑龙江畜牧兽医,2016,9:187-189. LI W,ZHOU L L,WANG D F,et al.Effect of areca nut extract on caecal histological structure in chickens infected by Eimeria tenella[J].Heilongjiang Animal Science and Veterinary Medicine, 2016,9:187-189.(in Chinese)
[14] 黎德兵,崔恒敏,赵翠燕.人工感染鸭疫里默氏杆菌病鸭主要器官的光镜及电镜观察[J].安徽农业科学,2008,36(15):6335-6337. LI D B,CUI H M,ZHAO C Y.Observation on main organs of the diseased duck artificially infected by Rimerella anatifer through light microscope and electron microscope[J].Journal of Anhui Agriculture Science,2008,36(15):6335-6337.(in Chinese)
[15] CHEN Z,XIE J,WANG B,et al.Effect of γ-aminobutyric acid on digestive enzymes,absorption function,and immune function of intestinal mucosa in heat-srtessed chicken[J].Poultry Science,2014,93(10):2490-500.
[16] 沈霞芬.家畜组织学与胚胎学[M].第3版.北京:中国农业出版社,2001. SHEN X F.Histology and Embryology of Domestic Animals[M].3rd Edition.Beijing:China Agriculture Press,2001.(in Chinese)
[17] LIM K H,STAUDT L M.Toll-like receptor signaling[J].Cold Spring Harb Perspectives Biology,2013,5(1):a011247.
[18] ARMANT M A,FENTON M J.Toll-like receptors:A family of pattern-recognition receptors in mammals[J].Genome Biology,2002,3(8):S3011.
[19] LU Y C,YEH W C,OHASHI P S.LPS/TLR4 signal transduction pathway[J].Cytokine,2008,42(2):145-151.
[20] COCHET F,PERI F.The Role of Carbohydrates in the lipopolysaccharide (LPS)/Toll-like receptor 4(TLR4) signalling[J].International Journal of Molecular Sciences,2017,18(11):E2318.
[21] VIJAY-KUMAR M,SANDERS C J,TAYLOR R T,et al.Deletion of TLR5 results in spontaneous colitis in mice[J].Journal of Clinical Investigation,2007,117(12):3909-3921.
[22] TIAN X,ZHAO H,ZHANG Z,et al.Intestinal mucosal injury induced by obstructive jaundice is associated with activation of TLR4/TRAF6/NF-κB pathways[J].PLoS One,2019,14(10):e0223651.
[23] HUANG X Y,ANSARI A R,HUANG H B,et al.Lipopolysaccharide mediates immuno-pathological alterations in young chicken liver through TLR4 signaling[J].BMC Immunology,2017,18(1):12.
[24] CHENG P,WANG T,LI W,et al.Baicalin alleviates lipopolysaccharide-induced liver inflammation in chicken by suppressing TLR4-mediated NF-κB pathway[J].Front Pharmacology,2017,8:547.
[25] LI R X,LI J,ZHANG S Y,et al.Attenuating effect of melatonin on lopopolysaccharide-induced chicken small intestine inflammation[J].Poultry Science,2018,97(7):2295-2302.
[26] LI N,ANSARI A R,SUN Z,et al.Toll like receptor 4 signaling pathway participated in salmonella lipopolysaccharide-induced spleen injury in young chicks[J].Microbial Pathogenesis,2017,112:288-294.
[27] KARNATI H K,PASUPULETI S R,KANDI R,et al.TLR-4 signalling pathway:MyD88 independent pathway up-regualtation in chicken breeds upon LPS treatment[J].Veternary Research Community,2015,39(1):73-78.

Effect of Riemerella anatipestifer on the Morphological Structure and TLR4 Signal Pathway Related Gene Expression of Cecum in Ducks

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