林蛙养殖场地土壤菌群结构及潜在病原菌分析

doi:10.16431/j.cnki.1671-7236.2018.02.030

Abstract

Abstract:

The aim of this study was to understand the composition and structural characteristics of soil microbial of Rana dybowskii, changes in microbes after spraying probiotics and the number of potential pathogens. Three experimental groups were set up:Breeding farm group,probiotics spraying group and semi-artificial breeding group. And high-throughput sequencing was used for detection. A total of 191 588 valid sequences and 1 369 OTUs were obtained from three experimental groups, which belonged to 438 genera and 29 phyla by taxonomy. The number of bacteria in three groups was relatively large, and the diversity level was relatively high. The dominant bacteria on phylum level were Proteobacteria, Bacteroidetes, Cyanobacteria, Gemmatimonadetes, Chloroflexi, Actinobacteria, Acidobacteria and Firmicutes;The dominant bacteria on genus level were Flavobacterium, Thermomonas, Sphingopyxis, Porphyrobacter and norank_c__Cyanobacteria. The diversity of microbial community among the groups did not reach the significant level. The similarity of the microbial communities was high. The potential red-leg syndrome pathogenic bacteria in the soil were Aeromonas, Citrobacter, Chryseobacterium and Pseudomonas. The changes of soil microbes after spraying probiotics were not obvious. This study provided the basis theory for comprehensively understanding the composition and variation of soil microbes of the Rana dybowskii farm, exploring the types of beneficial microorganisms and the prevention and treatment of Rana dybowskii.

Key words: Rana dybowskii; soil; high-throughput sequencing; microbial

CLC Number:

S154.38⁺1

BIE Jia, TONG Qing, HU Zongfu, CUI Liyong, LIU Xiaoning, WANG Hongbin. Analysis of Soil Microbial Communities and Potential Pathogens in Rana dybowskii Farm[J]. , 2018, 45(2): 528-535.

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Analysis of Soil Microbial Communities and Potential Pathogens in Rana dybowskii Farm

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