4种清热解毒复方中草药体外抑菌及抗PRRSV活性研究

doi:10.16431/j.cnki.1671-7236.2022.02.037

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the effects of 4 heat clearing and detoxifying compound Chinese herbal medicines, Xiexin decoction, Sanhuang Huzhang decoction, Huanglian Jiedu decoction and Qingwen Baidu powder on inhibiting Escherichia coli and Staphylococcus aureus combined with anti Porcine reproductive and respiratory syndrome virus (PRRSV) in vitro, in order to provide reference to clinicuse.【Method】 Active ingredients of 4 kinds of compoud Chinese herbal medicines were extracted by the conventional decoction method.Double dilution method was used to determine the in vitro antibacterial activities of 4 kinds of Chinese herbal medicines and their compound against Escherichia coli standard strain ATCC 259222 and Staphylococcus aureus standard strain ATCC 25923.Based on the study of the maximum safe concentration of Chinese herbal medicine on Marc-145 cells, the inhibitory effect of compound traditional Chinese medicine on PRRSV was determined by Real-time quantitative PCR.【Result】 Xiexin decoction had the best antibacterial activity against Escherichia coli in vitro, its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were both 7.81 mg/mL, followed by Sanhuang Huzhang decoction and Huanglian Jiedu decoction, which MIC and MBC were 31.25-125 mg/mL.Qingwen Baidu powder had poor inhibitory effect on Escherichia coli, the MIC and MBC were more than 250 mg/mL, while Staphylococcus aureus was very sensitive to Xiexin decoction, Huanglian Jiedu decoction and Sanhuang Huzhang decoction.The MIC and MBC were 0.24-0.98 mg/mL.In the experiment of anti PRRSV effect in vitro, the maximum safe concentrations of Xiexin decoction, Sanhuang Huzhang decoction, Huanglian Jiedu decoction and Qingwen Baidu powder on Marc-145 cells were 0.49, 0.49, 3.90 and 1.95 mg/mL, respectively.4 compound Chinese herbal medicines had good inhibitory and direct inactivation effects on PRRSV.Huanglian Jiedu decoction and Qingwen Baidu powder had blocking effects on PRRSV at 0.98 mg/mL, while Xiexin decoction and Sanhuang Huzhang decoction had blocking effects on PRRSV at 0.24 and 0.12 mg/mL, respectively.【Conclusion】 It was suggested that among 4 compound Chinese herbal medicines, Xiexin decoction had the best antibacterial and anti PRRSV effects in vitro, followed by Sanhuang Huzhang decoction, Huanglian Jiedu decoction and Qingwen Baidu powder.4 heat clearing and detoxifying compounds had certain antibacterial and antiviral activities, which could provide reference basis for clinical application.

Key words: Xiexin decoction; Sanhuang Huzhang decoction; Huanglian Jiedu decoction; Qingwen Baidu powder; in vitro antimicrobial; Porcine reproductive and respiratory syndrome virus (PRRSV)

CLC Number:

S852.65⁺1

SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.

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Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro

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