龙胆草及其提取物体外抗猪繁殖与呼吸综合征病毒作用的研究

doi:10.16431/j.cnki.1671-7236.2019.11.036

Abstract

Abstract: In order to study the water extract,total flavonoids and total glycosides of gentian in vitro against porcine reproductive and respiratory syndrome virus (PRRSV),the effect of Marc-145 cell model was established in this experiment.Based on the study of its maximum safe concentration of Marc-145 cells,combined with the observation of cytopathic morphology and MTT method,the drug group,ribavirin positive control group,virus control group and cell control group were set up to determine the three action modes of blocking,inhibition and direct inactivation to the virus,respectively.The results showed that the maximum safe concentrations of the water extract of gentian,total flavonoids of gentian,total glycosides of gentian and ribavirin were 6.250,0.020,0.630 and 0.016 mg/mL,respectively. In vitro anti-PRRSV effect of the test,the highest inhibition rates of the water extract of gentian on virus blocking,inhibition and direct inactivation effect were 43.1%,57.4% and 56.4%,respectively,which were all lower than that of ribavirin in the positive control group,and the cells had obvious pathological changes in each mode of action.The highest inhibition rates of the total flavonoids of gentian on virus blocking,inhibition and direct inactivation effect were 66.1%,41.1% and 42.7%,respectively.The inhibition rate of its inhibitory effect and direct inactivation effect were lower than that of ribavirin in the positive control group.The highest inhibition rates of the total glycosides of gentian on virus blocking,inhibition and direct inactivation effect were 33.4%,78.2% and 81.9%,respectively.The inhibition and direct inactivation were much stronger than that of ribavirin in the positive control group,and the cells basically kept the monolayer intact under the two action modes.Combined with the three action modes,among the three gentian extracts,the total glycosides of gentian had the best anti-PRRSV effect,followed by the water extract and the total flavonoids of gentian.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); cytopathic effect; antiviral activity; gentian

CLC Number:

S859.3

LIU Yun, ZHU Shanyuan, GONG Zhunan, QIN Feng, GU Lingling, WU Xiaojie. Study on the Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Gentian and Its Extract[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3449-3456.

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Study on the Anti-porcine Reproductive and Respiratory Syndrome Virus Effect of Gentian and Its Extract

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