纳米银体外抗猪繁殖与呼吸综合征病毒的作用研究

doi:10.16431/j.cnki.1671-7236.2022.11.026

Abstract

Abstract: 【Objective】 This study was aimed to study the antiviral effects of silver nanoparticles (AgNPs) on Porcine reproductive and respiratory syndrome virus (PRRSV) in vitro, and preliminarily analyze its mechanism, so as to provide a new idea for the prevention and control of PRRSV.【Method】 The safe concentration of AgNPs for subsequent experiments was selected from 0.1875, 0.375, 0.75, 1.5, 3, 6 and 12 μg/mL by CCK-8 kit which was used to evaluate the cytotoxicity of AgNPs to Marc145 cells.The antiviral effects of AgNPs on PRRSV were evaluated by microscopic observation, indirect immunofluorescence assay, virus titer determination and Real-time quantitative RT-PCR.Direct inactivation effect of AgNPs on PRRSV was evaluated by indirect immunofluorescence assay and Real-time quantitative RT-PCR.The effects of AgNPs on the adhesion and invasion of different multiplicity of infection (MOI) of PRRSV (0.0001 to 0.1) to Marc145 cells were analyzed by Real-time quantitative RT-PCR.The effects of AgNPs on PRRSV proliferation were analyzed by Real-time quantitative PCR after Marc145 cells were treated with AgNPs at the indicated time points that infected with PRRSV for 3, 6, 12, 18 and 24 h.【Result】 The maximum safe concentration of AgNPs to Marc145 cells was 1.5 μg/mL.There were both a certain inhibitory and inactivation effect of 0.375, 0.75 and 1.5 μg/mL AgNPs on PRRSV.The adhesion and invasion of different MOI strains to Marc145 cells was inhibited by AgNPs, and the proliferation of PRRSV was inhibited by adding AgNPs at 3, 6, 12, 18 and 24 h.【Conclusion】 AgNPs was anti-PRRSV, and the resistance was exerted through direct inactivation of PRRSV and inhibition of adhesion, invasion and proliferation of virus.

Key words: silver nanoparticles(AgNPs); Porcine reproductive and respiratory syndrome virus (PRRSV); antiviral activity; action mechanism

CLC Number:

S852.65⁺9.6

CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.

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Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro

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