基于F和HN蛋白的基因Ⅶ型新城疫病毒嵌合疫苗的构建及免疫效力评估

doi:10.16431/j.cnki.1671-7236.2022.11.025

Abstract

Abstract: 【Objective】 This study was aimed to construct a genotype Ⅶ Newcastle disease virus (NDV) chimeric vaccine, and access its immune efficacy.【Method】 The F and HN protein ectodomains of recombinant plasmid pT7-Y2 of the Avian metaavulavirus-2 (AMAV-2) Y2 strain were replaced with the corresponding F and HN protein ectodomains of genotype Ⅶ NDV HB0901 strain by utilizing reverse genetic technology.Then F protein cleavage site of HB0901 strain was transformed into that of lentogenic LaSota strain, and the chimeric virus was recovered and named rY2-FHNR.The growth characteristics, pathogenicity and genetic stability of rY2-FHNR strain were evaluated, then 2-week-old SPF chickens were immunized with rY2-FHNR to determine the immunogenicity and cross-reactivity between immune serum and Y2 strain.The immune serum was identified by indirect NDV NP ELISA to verify its diagnosis effect.【Result】 Chimeric recombinant virus strain rY2-FHNR was constructed successfully.The biological characteristics results showed that the proliferation titer and pathogenicity in chicken embryos of rY2-FHNR strain were avirulent, while the chimeric virus was genetic stable when serially passaged in chicken embryos.Immunization with rY2-FHNR stimulated antibodies against NDV, and the immune serum had no cross-reactivity with rY2 strain.Through indirect NDV NP ELISA could distinguish infected animals from vaccinated animals.【Conclusion】 This study developed a genotype Ⅶ NDV chimeric vaccine candidate, which could be used as chimeric vaccine, provided technological support for the monitoring, prevention and eradication of genotype Ⅶ NDV.

Key words: Newcastle disease virus (NDV); genotype Ⅶ; Avian metaavulavirus-2(AMAV-2); reverse genetic technology; chimeric vaccine

CLC Number:

S852.65⁺7

LI Li, WANG Guokang, SHANG Yu, XU Yingying, FENG Helong, WANG Hongcai, ZHANG Rongrong, ZENG Zhe, LUO Qingping, SHAO Huabin, ZENG Chi, WEN Guoyuan. Construction and Immune Efficacy Evaluation of a Genotype Ⅶ Newcastle Disease Virus Chimeric Vaccine Based on F and HN Proteins[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4346-4355.

References

[1] NAGUIB M M, HÖPER D, ELKADY M F, et al.Comparison of genomic and antigenic properties of Newcastle disease virus genotypes Ⅱ, ⅩⅪ and Ⅶ from Egypt do not point to antigenic drift as selection marker[J].Transboundary and Emerging Diseases, 2022, 69(2):849-863.
[2] SAHOO N, BHUYAN K, PANDA B, et al.Prevalence of Newcastle disease and associated risk factors in domestic chickens in the Indian state of Odisha[J].PLoS One, 2022, 17(2):e0264028.
[3] SULTAN H A, ELFEIL W K, NOUR A A, et al.Efficacy of the Newcastle disease virus genotype Ⅶ.1.1-Matched vaccines in commercial broilers[J].Vaccines, 2021, 10(1):29.
[4] JIA W F, ZHANG X H, WANG H R, et al.Construction and immune efficacy of a recombinant Turkey herpesvirus vaccine strain expressing fusion protein of genotype Ⅶ Newcastle disease virus[J].Veterinary Microbiology, 2022, 268:109429.
[5] RIMA B, BALKEMA-BUSCHMANN A, DUNDON W G, et al.ICTV virus taxonomy profile:Paramyxoviridae[J].Journal of General Virology, 2019, 100(12):1593-1594.
[6] AMARASINGHE G K, AYLLON M A, BAO Y, et al.Taxonomy of the order Mononegavirales:Update 2019[J].Archives of Virology, 2019, 164(7):1967-1980.
[7] DIMITROV K M, ABOLNIK C, AFONSO C L, et al.Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus[J].Infection, Genetics and Evolution, 2019, 74:103917.
[8] SHIN-HEE K, SA X, HEATHER S, et al.Replication, neurotropism, and pathogenicity of Avian paramyxovirus serotypes 1-9 in chickens and ducks[J].PLoS One, 2012, 7(4):e34927.
[9] STEGLICH C, GRUND C, RAMP K, et al.Chimeric Newcastle disease virus protects chickens against avian influenza in the presence of maternally derived NDV immunity[J].PLoS One, 2013, 8(9):e72530.
[10] SUBBIAHA M, XIAOA S, KHATTARA S K, et al.Pathogenesis of two strains of Avian paramyxovirus serotype 2, Yucaipa and Bangor, in chickens and turkeys[J].Avian Diseases, 2010, 54(3):1050-1057.
[11] TSUNEKUNI R, HIKONO H, TANIKAWA T, et al.Recombinant Avian paramyxovirus serotypes 2, 6, and 10 as vaccine vectors for highly pathogenic avian influenza in chickens with antibodies against Newcastle disease virus[J].Avian Diseases, 2017, 61(3):296-306.
[12] 郭丽丽, 廖天星, 高晓敏, 等.新城疫病毒HN标记疫苗的构建及免疫效力[J].中国家禽, 2020, 42(2):21-25. GUO L L, LIAO T X, GAO X M, et al.Construction and immune efficacy of one HN marker vaccine against Newcastle disease virus infection[J].China Poultry, 2020, 42(2):21-25.(in Chinese)
[13] 王国康, 李丽, 贾妙妙, 等.基于全基因组序列合成的禽副黏病毒Ⅱ型反向遗传操作系统的建立[J].中国兽医学报, 2022, 42(3):464-471. WANG G K, LI L, JIA M M, et al.Establishment of reverse genetic system of Avian paramyxovirus type Ⅱ based on the whole genome synthesis[J].Chinese Journal of Veterinary Science, 2022, 42(3):464-471.(in Chinese)
[14] 贾妙妙, 王国康, 李丽, 等.基于新城疫病毒NP蛋白的间接ELISA抗体检测方法的建立[J].中国兽医学报, 2021, 41(3):475-479. JIA M M, WANG G K, LI L, et al.Development of an indirect ELISA for NDV antibody detection based on NP protein[J]. Chinese Journal of Veterinary Science, 2021, 41(3):475-479.(in Chinese)
[15] EDWARDS S.The work of the OIE biological standards commission[J].Developmental Biology, 2007, 128:5-11.
[16] FRANCISCO L, BAILEY S, BAUTISTA T, et al.Detection of velogenic Avian paramyxoviruses in rock doves in New York city, New York[J].Microbiology Spectrum, 2022, 10:e0206121.
[17] FAWZY M, ALI R R, ELFEIL W K, et al.Efficacy of inactivated velogenic Newcastle disease virus genotype Ⅶ vaccine in broiler chickens[J].Veterinary Research Forum, 2020, 11(2):113-120.
[18] JI Y H, LIU T, DU Y Y, et al.A novel genotype Ⅶ Newcastle disease virus vaccine candidate generated by mutation in the L and F genes confers improved protection in chickens[J].Veterinary Microbiology, 2018, 216(8):99-106.
[19] AHMAD-RAUS R, ALI A M, WEN S T, et al.Localization of the antigenic sites of Newcastle disease virus nucleocapsid using a panel of monoclonal antibodies[J].Research in Veterinary Science, 2009, 86(1):174-182.
[20] MEBATSION T, KOOLEN M, VAAN L D, et al.Newcastle disease virus (NDV) marker vaccine:An immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope[J].Journal of Virology, 2002, 76(20):10138-10146.
[21] BI Y, JIN Z, WANG Y, et al.Identification of two distinct linear B cell epitopes of the matrix protein of the Newcastle disease virus vaccine strain LaSota[J].Viral Immunology, 2019, 32(5):221-229.
[22] UTTENTHAL A, PARIDA S, RASMUSSEN T B, et al.Strategies for differentiating infection in vaccinated animals (DIVA) for foot-and-mouth disease, classical swine fever and avian influenza[J].Expert Review of Vaccines, 2010, 9(1):73-87.
[23] KIM S H, SUBBIAH M, SAMUEL A S, et al.Roles of the fusion and hemagglutinin-neuraminidase proteins in replication, tropism, and pathogenicity of Avian paramyxoviruses[J].Journal of Virology, 2011, 85(17):8582-8596.
[24] SHIRVANI E, SAMALS K, Comparative protective efficacies of novel Avian paramyxovirus-vectored vaccines against virulent Infectious bronchitis virus in chickens[J].Viruses, 2020, 12(7):697.

Construction and Immune Efficacy Evaluation of a Genotype Ⅶ Newcastle Disease Virus Chimeric Vaccine Based on F and HN Proteins

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