核苷二磷酸激酶B稳定表达细胞系的建立及其对新城疫病毒F48E9株复制的影响

doi:10.16431/j.cnki.1671-7236.2017.08.010

Abstract

Abstract:

In order to explore the influence of nucleoside diphosphate kinase B (NDPK B) on replication and proliferation of F48E9 strain of Newcastle disease virus (NDV), the eukaryotic expression plasmid pEGFP-NDPK B was transfected into Vero cells,then we got the cell subclones by G418 screening. We identified the expression of NDPK B protein by RT-PCR, Western blotting and inverted flurescence microscope. On the basis of Vero/NDPK B cell line,we explored the influence of NDPK B on replication and proliferation of F48E9 strain of NDV by HA-HI, TCID₅₀ and Real-time PCR.The results showed that we had successfully constructed the cell line named Vero/NDPK B, which could stably express NDPK B protein, and found that NDPK B could inhibit the replication and proliferation of F48E9 strain of NDV in Vero cells.It suggested that on the basis of NDPK B,we could design and develop new drugs or antiviral synergist to prevent or treat NDV.

Key words: nucleoside diphosphate kinase B (NDPK B); expression; Newcastle disease virus (NDV); replication and proliferation

CLC Number:

S852.2

GUO Yu-pei, BAN Fu-guo, YAN Ruo-qian, FANG Xian-zhen, DING Zhuang. Establishment of a Stable Expression Cell Line of Nucleoside Diphosphate Kinase B and Its Influence on Replication of F48E9 Strain of Newcastle Disease Virus[J]. , 2017, 44(8): 2283-2288.

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Establishment of a Stable Expression Cell Line of Nucleoside Diphosphate Kinase B and Its Influence on Replication of F48E9 Strain of Newcastle Disease Virus

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