添加通路阻断剂对IGF-1影响奶牛乳腺上皮细胞增殖与凋亡的研究

doi:10.16431/j.cnki.1671-7236.2017.08.009

Abstract

Abstract:

The test was aimed to study the effect of insulin-like growth factor 1(IGF-1) on the proliferation of bovine mammary epithelial cells (BMECs),and provide a theoretical basis for the subsequent regulating mammary gland development using IGF-1. The optimal IGF-1 concentration for inhibiting BMECs apoptosis was obtained by measuring the apoptosis rate at 12, 24, 48 and 72 h after the addition of IGF-1 at four groups (0, 10, 50 and 100 μg/mL). Then divided into six groups:BMECs group, BMECs+IGF-1 group, BMECs+LY294002 group, BMECs+IGF-1+LY294002 group, BMECs+RAPA group and BMECs+IGF-1+RAPA group,and the apoptosis rates were detected by flow cytometry. The results showed that the heterologous IGF-1 had an inhibitory effect on the apoptosis of BMECs with an optimal concentration of 50 μg/mL. The apoptosis rates of BMECs+IGF-1+LY294002 and BMECs+IGF-1+RAPA groups were extremely significantly higher than BMECs+IGF-1 group (P<0.01). This study suggested that IGF-1 could activate the PI3K/Akt/mTOR signaling pathway and thereby inhibit the apoptosis of BMECs. Moreover, IGF-1 might also promote the "repair" mechanism for the inhibited PI3K/Akt/mTOR signaling pathway, and therefore make it reparticipate in BMECs life process.

Key words: insulin-like growth factor 1(IGF-1); bovine mammary epithelial cells (BMECs); proliferation; apoptosis

CLC Number:

Q253

LUO Cheng-long, ZHAO Yan-kun, WU Kai-le, SHAO Wei. Study on Effect of IGF-1 on Proliferation and Apoptosis of Bovine Mammary Epithelial Cells by Adding Inhibitor[J]. , 2017, 44(8): 2277-2282.

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Study on Effect of IGF-1 on Proliferation and Apoptosis of Bovine Mammary Epithelial Cells by Adding Inhibitor

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