连花柴芩可溶性粉对鸡风热犯肺证的临床试验研究

doi:10.16431/j.cnki.1671-7236.2022.09.035

Abstract

Abstract: 【Objective】 This experiment was aimed to study the clinical effect of Lianhuachaiqin soluble powder on fever, cough, anorexia, thirst and drinking in chickens caused by wind-heat invading lung.【Method】 The broiler model of wind-heat invading lung syndrome was established by artificial challenge of chicken Infectious bronchitis virus.150 chickens with typical clinical symptoms of wind-heat invading lung syndrome were included in the experiment, and were randomly divided into 5 groups:Lianhuachaiqin soluble powder high, medium, low dose groups (Lianhuachaiqin soluble powder concentrations were 4, 2, 1 g/L, respectively), Ma Xing Shigan granule control group (1 g/L), and model control group, 30 chickens in each group.Another 30 chickens were used as healthy control group.Except for the healthy control group and the model group, the experimental groups were treated with drinking water continuously for 5 days.The changes of mental state, body temperature, respiratory symptoms, intake and drinking water of chickens before and after administration were recorded, and the indexes of immune organs and immune cell subsets in peripheral blood of chickens in each group were detected to comprehensively investigate the clinical effect of Lianhuachaiqin soluble powder.【Result】 The total effective rates of high and medium dose groups of Lianhuachaiqin soluble powder on fever and cough caused by wind-heat invading lung were 90.00% and 86.66% respectively, which were significantly higher than those in the model control group (P<0.05).And it had a good alleviating effect on the decrease of feed intake and the increase of drinking water in diseased chickens (P<0.05).On the other hand, the Lianhuachaiqin soluble powder could increase the immune organ index and promote the proliferation of CD3⁺, CD4⁺ and CD8⁺ T lymphocytes in the peripheral blood of diseased chickens, and the high and medium doses were significantly higher than those in the model control group (P<0.05).【Conclusion】 The results showed that Lianhuachaiqin soluble powder had good relieving and therapeutic effect on chicken fever, cough, loss of appetite and thirst caused by wind-heat invading lung. Combined with drug dose-effect relationship and economic cost, it was recommended that the clinical dosage of Lianhuachaiqin soluble powder was 2 g/L, given in drinking water for 5 days.

Key words: Lianhuachaiqin soluble powder; chicken syndrome of wind-heat invading lung; clinical efficacy; lymphocyte proliferation

CLC Number:

S853.74

WANG Xuefang, GUI Rong, CHEN Gongyi, WANG Wei, ZHANG Hongying, WANG Xuebing, LI Xiao. Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3610-3621.

References

[1] GALLARDO R A.Infectious bronchitis virus variants in chickens:Evolution, surveillance, control and prevention[J].Austral Journal of Veterinary Sciences, 2021, 53(1):55-62.
[2] 闫延华, 陈彤, 陈泽文, 等.中药复方板青可溶性粉防治鸡传染性支气管炎的效果研究[J].中国畜牧兽医, 2020, 47(10):3379-3388. YAN Y H, CHEN T, CHEN Z W, et al.Study on the preventive and therapeutic effect of compound Banqing soluble powder on chicken infectious bronchitis[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(10):3379-3388.(in Chinese)
[3] 杨彦文, 李梓密, 刘群.双黄连可溶性粉不同组分抗菌抗病毒活性研究[J].中国畜牧兽医, 2021, 48(8):3087-3096. YANG Y W, LI Z M, LIU Q.Study on antibacterial and antiviral activity of different components of Shuanghuanglian soluble powder[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(8):3087-3096.(in Chinese)
[4] 冯海鹏, 辛蕊华, 张凯, 等.中兽药复方组合与抗生素防治肉鸡呼吸道疾病的协同效应[J].中国畜牧兽医, 2020, 47(3):940-947. FENG H P, XIN R H, ZHANG K, et al.Synergistic effect of combination of Chinese veterinary drugs and antibiotics on the prevention and treatment of respiratory diseases in broilers[J].China Animal Husbandry & Veterinary Medicine, 2020, 47(3):940-947.(in Chinese)
[5] 裴智勇.鸡呼吸道疾病的辨证施治[J].中国畜牧兽医文摘, 2015, 31(6):192. PEI Z Y.Syndrome differentiation and treatment of respiratory diseases in chickens[J].Chinese Abstracts of Animal Husbandry and Veterinary Medicine, 2015, 31(6):192.(in Chinese)
[6] 齐丽娜, 陈炫好, 金华, 等.中药连翘化学成分及药理活性研究进展[J].天津中医药大学学报, 2021, 40(2):168-175. QI L N, CHEN X H, JIN H, et al.Research progress on chemical constituents and pharmacological activity of Forsythia suspensa[J].Journal of Tianjin University of Traditional Chinese Medicine, 2021, 40(2):168-175.(in Chinese)
[7] 夏伟, 董诚明, 杨朝帆, 等.连翘化学成分及其药理研究进展[J].中国现代中药, 2016, 18(12):1670-1674. XIA W, DONG C M, YANG C F, et al.Research progress on chemical constituents and pharmacology of Forsythia suspensa[J].Modern Chinese Medicine, 2016, 18(12):1670-1674.(in Chinese)
[8] 陈梦莹, 李钦, 韩晶晶, 等.老翘的化学成分研究[J].天然产物研究与开发, 2020, 32(12):2066-2072. CHEN M Y, LI Q, HAN J J, et al.Chemical constituents of grown Fructus forsythiae[J].Natural Product Research and Development, 2020, 32(12):2066-2072.(in Chinese)
[9] TANG Y R, ZENG T, SALMAN ZAFAR, et a1.Lonicerae flos:A review of chemical constituents and biological activities[J].Digital Chinese Medicine, 2018, 1(2):173-188.
[10] 颜美玲, 杨柳, 侯阿娇, 等.柴胡化学成分及药理作用研究进展[J].中医药信息, 2018, 35(5):103-109. YAN M L, YANG L, HOU A J, et a1.Research progress on chemical constituents and pharmacological effects of Bupleurum[J]. Information on Traditional Chinese Medicine, 2018, 35(5):103-109.(in Chinese)
[11] 马玲玲, 孙燕.中药黄芩药理作用的研究进展[J].沈阳医学院学报, 2016, 18(2):115-117. MA L L, SUN Y.Research progress in pharmacological effects of traditional Chinese medicine Radix Scutellariae[J].Journal of Shenyang Medical College, 2016, 18(2):115-117.(in Chinese)
[12] 朱亚南, 杨七妹, 张硕, 等.黄芩苷与黄芩素药理作用及机制研究进展[J].时珍国医国药, 2020, 31(4):921-925. ZHU Y N, YANG Q M, ZHANG S, et a1.Advances in pharmacological effects and mechanisms of baicalin and baicalein[J].Lishizhen Medicine and Materia Medica Research, 2020, 31(4):921-925.(in Chinese)
[13] 钟萍, 汪镇朝, 刘英孟, 等.紫苏叶挥发油化学成分及其药理作用研究进展[J].中国实验方剂学杂志, 2021, 27(13):215-225. ZHONG P, WANG Z C, LIU Y M, et a1.Advances on chemical constituents of essential oils from Perillae folium and their pharmacological effect:A review[J].Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27(13):215-225.(in Chinese)
[14] 陈健敏, 黄玮玥, 叶雅玲, 等.甘草提取物的功效及其作用机制研究[J].长江大学学报(自然版), 2019, 16(7):109-115. CHEN J M, HUANG W Y, YE Y L, et a1.Study on the efficacy and action mechanism of licorice extract[J].Journal of Yangtze University (Natural Science Edition), 2019, 16(7):109-115.(in Chinese)
[15] 刘钟杰, 许剑琴.中兽医学[M].北京:中国农业出版社, 2009. LIU Z J, XU J Q.Chinese Veterinary Medicine[M].Beijing:China Agriculture Press, 2009.(in Chinese)
[16] 钟秀会, 马爱团.中兽医基础理论[M].北京:中国农业出版社, 2011. ZHONG X H, MA A T.Basic Theory of Chinese Veterinary Medicine[M].Beijing:China Agriculture Press, 2011.(in Chinese)
[17] 风温肺热病的诊断依据、证候分类、疗效评定标准——中华人民共和国中医药行业标准《中医内科病证诊断疗效标准》(ZY/T001.1-94)[J].辽宁中医药大学学报, 2013, 15(4):7. Diagnostic basis, syndrome classification and therapeutic effect evaluation standard of wind-warming lung-heat disease——Industrial standard of traditional Chinese medicine of the People's Republic of China "Criteria of diagnosis and therapeutic effect of internal diseases and syndromes in traditional Chinese medicine"(ZY/T001.1-94)[J].Journal of Liaoning University of Traditional Chinese Medicine, 2013, 15(4):7.(in Chinese)
[18] 郑筱萸.中药新药临床研究指导原则(试行)[M].北京:中国医药科技出版社, 2002. ZHENG Y Y.Guiding Principles for Clinical Research of New Traditional Chinese Medicine (Trial)[M].Beijing:China Medical Science and Technology Press, 2002.(in Chinese)
[19] 农业部兽药评审中心.兽药研究技术指导原则汇编(2006-2011)[M].北京:化学工业出版社, 2012. VETERINARY DRUG EVALUATION CENTER OF THE MINISTRY OF AGRICULTURE.Compilation of Technical Guidelines for Veterinary Drug Research(2006-2011)[M].Beijing:Chemical Industry Press, 2012.(in Chinese)
[20] 邓锐涵, 周紫冲, 杨艳艳, 等.金芩蓝口服液对鸡风热犯肺证的疗效研究[J].中国兽药杂志, 2021, 55(2):46-52. DENG R H, ZHOU Z C, YANG Y Y, et al.Study on the effect of Jinqinlan oral liquid on chicken syndrome of wind-heat invading lung[J].Chinese Journal of Veterinary Drug, 2021, 55(2):46-52.(in Chinese)
[21] 齐有胜, 孙毅坤, 刘为萍.单味中药抗流感病毒研究进展[J].中国实验方剂学杂志, 2017, 23(14):210-218. QI Y S, SUN Y K, LIU W P.Research progress of single Chinese herbal medicine against Influenza virus[J].Chinese Journal of Experimental Traditional Medical Formulae, 2017, 23(14):210-218.(in Chinese)
[22] 赵冰倩, 罗畅, 刘健新, 等.连翘水提液体外对禽流感病毒增殖及炎症因子表达的抑制效应[J].畜牧兽医学报, 2020, 51(6):1466-1474. ZHAO B Q, LUO C, LIU J X, et al.Inhibitory effect of Forsythiae fructus aqueous extracts on the proliferation of Avian influenza virus and the expression of inflammation factors in vitro[J].Chinese Journal of Animal and Veterinary Sciences, 2020, 51(6):1466-1474.(in Chinese)
[23] 张羽璐, 李华伟, 庄英华, 等.连翘酯苷对人工感染传染性支气管炎病毒雏鸡肺组织JAK/STAT信号通路的调节作用[J].北京农学院学报, 2011, 26(2):21-23. ZHANG Y L, LI H W, ZHUANG Y H, et al.Effect of forsythiaside on activation of JAK/STAT pathway in the lung from the chicken infected by Infectious bronchitis virus[J].Journal of Beijing University of Agriculture, 2011, 26(2):21-23.(in Chinese)
[24] 张彤, 刘蓓桦, 杨晓炼, 等.连翘酯苷A对IBV感染细胞内受体和抗病毒基因表达的影响[J].北京农学院学报, 2017, 32(1):37-42. ZHANG T, LIU B H, YANG X L, et al.Effect of forsythoside A on expression of intracellular receptors and antiviral gene in IBV-infected cells[J].Journal of Beijing University of Agriculture, 2017, 32(1):37-42.(in Chinese)
[25] 陈灵, 周艳萌, 欧水平, 等.山银花水提物体外抗甲型H1N1流感病毒的作用研究[J].中国药房, 2017, 28(16):2194-2197. CHEN L, ZHOU Y M, OU S P, et al.Study on the anti-influenza A virus H1N1 effect of aqueous extraction of Lonicerae flos in vitro[J].China Pharmacy, 2017, 28(16):2194-2197.(in Chinese)
[26] 王林青, 张红英, 崔保安, 等.金银花、山银花绿原酸类提取物体外抗NDV作用研究[J].中国农学通报, 2011, 27(19):277-282. WANG L Q, ZHANG H Y, CUI B A, et al.The study on antiviral effect of chlorogenic acids from Flos lonicerae japonicae and Flos lonicerae on NDV in vitro[J].Chinese Agricultural Science Bulletin, 2011, 27(19):277-282.(in Chinese)
[27] 邹珊珊, 玄振玉.连翘酯苷不同给药途径对副流感病毒致家兔发热模型的影响[J].中药药理与临床, 2015, 31(1):57-59. ZOU S S, XUAN Z Y.Studies of different administration routes of forsythiaside on rabbit fever models induced by Parainfluenza virus[J].Pharmacology and Clinics of Chinese Materia Medica, 2015, 31(1):57-59.(in Chinese)
[28] 李兴平, 白筱璐, 雷玲, 等.金银花的解热作用[J].中药药理与临床, 2012, 28(2):37-40. LI X P, BAI X L, LEI L, et al.Antipyretic effect of honeysuckle[J].Pharmacology and Clinics of Chinese Materia Medica, 2012, 28(2):37-40.(in Chinese)
[29] 杨江萍, 高增平, 谢鸣.柴芩水提液退热有效部位的研究[J].中国实验方剂学杂志, 2012, 18(14):131-134. YANG J P, GAO Z P, XIE M.Study on antifebrile parts for Chaiqin decoction[J].Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(14):131-134.(in Chinese)
[30] 薛洋, 闫延华, 付敬敬, 等.黄芩苷防治雏鸡传染性支气管炎的药理作用[J].江苏农业学报, 2017, 33(5):1082-1092. XUE Y, YAN Y H, FU J J, et al.Pharmacology of prevention and control of avian infectious bronchitis by baicalin[J].Jiangsu Journal of Agricultural Sciences, 2017, 33(5):1082-1092.(in Chinese)
[31] 崔小七, 范云鹏, 郑寅, 等.柴黄颗粒对人工感染鸡传染性支气管炎的防治研究[J].中国家禽, 2013, 35(16):25-28. CUI X Q, FAN Y P, ZHENG Y, et al.Prevention and treatment of Chaihuang granule on chickens infected artificially with avian infectious bronchitis[J].China Poultry, 2013, 35(16):25-28.(in Chinese)

Clinical Study of Lianhuachaiqin Soluble Powder on Chicken Syndrome of Wind-heat Invading Lung

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	QIAN Feng, MIAO Yusong, MUHAMMAD Ishfaq, YIN Peng, YIN Jianli, LI Jichang. Pharmacodynamics of Baiqin Extract and Clinical Efficacy Evaluation for Treatment of Cow Endometritis [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1618-1627.
[2]	YU Wenhui, LI Shuhong, JIANG Xiaowen, CHEN Yanan, ZHONG Qiu, HUANG Hui. Study on Antimicrobial Activity and Clinical Efficacy of Coptis chinensis Prescription on E.coli O₇₈ [J]. , 2018, 45(3): 814-821.
[3]	ZHANG Fuxin, SONG Jiaxuan, LIU Xiaodong. Antioxidation and Immune Activity of Oxytropis kansuensis flavonoids [J]. , 2018, 45(12): 3387-3394.
[4]	GUAN Yuan-hong, FU Hong-qing, ZOU Zhi-heng, WEI Qi-peng. Study on Effects of 3 Polysaccharides on Chicken Peripheral Lymphocyte and Spleen Lymphocyte Proliferation in Vitro [J]. , 2014, 41(12): 177-181.