黄连组方对大肠杆菌O78的抑菌作用与临床疗效研究

doi:10.16431/j.cnki.1671-7236.2018.03.032

Abstract

Abstract:

To study the new method for prevention and treatment of chicken colibacillosis and reduce the use of antibiotics and drug-resistant strains,Coptis chinensis,Fructus mume,Atractylodes and other traditional Chinese medicine were selected to constitute the Coptis chinensis prescription oral liquid.The inhibitory effect and clinical efficacy of Coptis chinensis prescription oral liquidon on E.coli O₇₈ were tested.MIC and MBC were detected to show the oral antibacterial effect in vitro test.The effects of oral liquid on bacterial mortality were observed by flow cytometry.Meanwhile the effects of oral liquid on the morphology and structure of the cells were observed by scanning electron microscopy and transmission electron microscopy,in vitro test.The effect of oral liquid on the treatment of colibacillosis in chickens and the index of immune organ in clinical trials were investigated.The results showed that MIC was 62.5 mg/mL,MBC was 250 mg/mL.The result of flow cytometry showed that the mortality rate of E.coli was 74.1% in the oral liquid group but it was 37.2% in blank control group.Scanning electron microscopy showed that the cells of the oral liquid treatment group were spilled and broken,and many debris were formed.Under the transmission electron microscope,the cell deformation and the wall separation were observed.Clinical efficacy showed that the cure rate was 86.67% in Coptis chinensis prescription,while the cure rate was 63.33% in the group administrated with norfloxacin.The spleen index of blank control group and western medicine group were extremely significantly different from that of model group (P < 0.01).The bursa index of blank control group,western medicine group and Coptis chinensis high dose group were extremely significantly higher than that of model group (P < 0.01).The thymus index of model group was significantly lower than that of other groups (P < 0.05).In a word,Coptis chinensis prescription could make the bacteria deformation,constriction fracture and wall separation to achieve the role of sterilization.Meanwhile,Coptis chinensis could enhance animals immune function to prescription prevention and treatment of chicks with E.coli disease.

Key words: Coptis chinensis prescription; E.coli O₇₈; bacteriostatic; chicks; clinical efficacy

CLC Number:

S853.74

YU Wenhui, LI Shuhong, JIANG Xiaowen, CHEN Yanan, ZHONG Qiu, HUANG Hui. Study on Antimicrobial Activity and Clinical Efficacy of Coptis chinensis Prescription on E.coli O₇₈[J]. , 2018, 45(3): 814-821.

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Study on Antimicrobial Activity and Clinical Efficacy of Coptis chinensis Prescription on E.coli O₇₈

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Study on Antimicrobial Activity and Clinical Efficacy of Coptis chinensis Prescription on E.coli O78

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Study on Antimicrobial Activity and Clinical Efficacy of Coptis chinensis Prescription on E.coli O₇₈