中药常山散的体外抑菌作用研究

doi:10.16431/j.cnki.1671-7236.2017.02.040

Abstract

Abstract:

The purpose of this study was to indicate the antibacterial effect of traditional Chinese medicine Radix dichroa powder (RDP) in vitro. Minimal inhibitory concentration (MIC) of RDP for 12 pathogens were determined by two fold dilution method and agar dilution method, and the dose-effect relationship of antibiotic effect was studied, and the antimicrobial effect was also observed by Oxford-cup method. The results from two fold dilution method indicated that RDP had stronger inhibited effect to Streptococcus and Bacillus subtilis (MIC were from 15.6 to 62.5 mg/mL) than that of S. aureus (MIC was about 250 mg/mL), Enterobacteria (MIC was about 500 mg/mL) and fungi (MIC > 500 mg/mL); The results of agar dilution method showed that RDP had some antibacterial activities on Proteusbacillus vulgaris, Candida albicans And aspergillus niger, MIC was about 500 mg/mL, while it had weak antibacterial effect on other pathogens (MIC > 500 mg/mL). The results of Oxford-cup method showed that RDP (500 mg/mL) had some degree antimicrobial effect on pathogens, and the bacteria-inhibiting ring diameter of RDP not exceeded 10 mm, while drug-action ring was observed obviously around the Oxford-cup, and the number of bacteria was significantly decreased within the ring. In conclusion, RDP had some degree antibacterial effect on common pathogens, but the effects were not strong because of charicteristics of RDP itself and lower effective content.

Key words: Radix dichroa powder (RDP); minimal inhibitory concentration (MIC); pathogen; bacteriostatic test

CLC Number:

S853.76

WANG Ling, GUO Zhi-ting, YANG Feng, WANG Wen-li, MO Ya-xia, GUO Ai-ming, LUO Xiao-qin, WEI Xiao-juan, LV Ya-nan. Bacteriostatic Test in vitro of Traditional Chinese Medicine Radix Dichroa Powder on Common Pathogens[J]. , 2017, 44(2): 594-600.

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Bacteriostatic Test in vitro of Traditional Chinese Medicine Radix Dichroa Powder on Common Pathogens

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