基于CRISPR-Cas系统的病原体检测研究进展

doi:10.16431/j.cnki.1671-7236.2022.08.035

Abstract

Abstract: Development of rapid,specific and sensitive detection method for emerging and re-emerging zoonosis and animal disease is critical for disease surveillance,prevention and control.CRISPR-Cas system,characterized by their sensitivity,specificity,and scalability upon DNA and RNA recognition,have been repurposed for the new generation of molecular diagnostic tools and are highly applied for pathogens detection.In the COVID-19 pandemic,CRISPR-Cas based SARS-CoV-2 detection kits (SHERLOCK and DETECTR) have been approved by USA,and CRISPR-Cas12 based SARS-CoV-2 detection kit developed in China has also been approved for marketing,implying that CRISPR-Cas assisted detection method could have a lot of uses in the future.This review firstly introduces the classification of CRISPR-Cas systems,especially CRISPR-Cas9/Cas12/Cas13,which are commonly used for pathogen detection. It also introduces the mechanism and the wider applications of CRISPR-Cas system. Meanwhile,the paper analyses the key technical issues in the development of CRISPR-Cas detection platform including sequence conservation of target genes,target enrichment and amplification,pathogens sensing with different signal readouts,and discusses the future development and application prospect of this method.

Key words: CRISPR-Cas system; pathogens detection; diagnosis; application

CLC Number:

R446.5

ZHANG Qingxun, ZHONG Zhenyu, GUO Qingyun, HE Hongxuan, BAI Jiade. Recent Advances of Pathogens Detection Based on CRISPR-Cas System[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3190-3199.

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Recent Advances of Pathogens Detection Based on CRISPR-Cas System

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