血清白蛋白的功能、应用及纯化工艺研究进展

doi:10.16431/j.cnki.1671-7236.2023.06.010

Abstract

Abstract: Albumin is an important component of serum.It is a highly water-soluble globular protein, which can maintain the balance of colloid osmotic pressure and has both anticoagulant and antioxidant effect.Serum albumin (SA) chemical structure and conformation allows interaction with many different drugs, affecting drugs transport and metabolic processes.As a protein carrier for endogenous and exogenous ligands, SA is of great clinical significance to be used to treat a variety of diseases, including hemorrhagic shock, hypoalbuminemia, chronic liver disease, etc.At the same time, it plays an important role in animal production by acting as cell culture agent, influencing the in vitro maturation of oocytes and the viability of frozen sperm.Therefore, the demand for SA increased annually worldwide, and it is crucial to obtain SA with high purity, high activity and high recovery.Currently, the purification process of SA includes ethyl alcohol precipitation, affinity precipitation, trichloroacetic acid/acetone precipitation method, ammonium sulfate precipitation combined with liquid chromatography, Cohn method combined with liquid chromatography, and chromatography.In order to clarify the extraction and purification process of SA, the structure, physiological functions, applications and purification processes of SA were reviewed in this paper, and the principles, purification effect, advantages, disadvantages of different purification processes were summarized.It was expected to provide a reference for the in-depth research, development, and utilization of SA, and further promotes the application of SA in the fields of medicine, molecular biology research and immuno-diagnostics.

Key words: serum albumin; function; application; purification process

CLC Number:

Q512⁺.1

WU Xuan, ZENG Shenming. Research Progress on the Function,Application and Purification Technology in Serum Albumin[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2265-2275.

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Research Progress on the Function,Application and Purification Technology in Serum Albumin

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