H9N2亚型禽流感病毒SD18株的传染性及不同途径感染效果比较

doi:10.16431/j.cnki.1671-7236.2022.04.027

Abstract

Abstract: 【Objective】 The study was aimed to elucidate the transmission and pathogenicity of H9N2 subtype Avian influenza virus (AIV) in mammals.【Method】 Samples were collected for virus isolation and identification in this study.The isolated virus was used to infect guinea pigs by nasal drops and lung delivery at a dose of 300 μL (10⁷ EID₅₀),with 15 guinea pigs in each group,and the infection effects of the two groups were compared,then the aerosol transmission ability of the isolated strain was verified by the transmission ability experiment in guinea pigs.The replication ability of the isolated strain on human bronchial epithelial cells was detected by indirect immunofluorescence assay.【Result】 A strain of H9N2 subtype AIV was isolated and was named SD18.After guinea pigs were infected with the virus by nasal drip and lung delivery,the guinea pigs infected by lung delivery showed greater virus excretion than the nasal drip (P<0.05).Through the detection the expression of pattern recognition receptors and antiviral proteins in lungs of guinea pigs,the expression of immunologic factors Toll-like receptor 3 (TLR3),TLR7,myxovirus-resistance (MX),2'-5'oligoadenylates synthesis (OAS) had extremely significantly increased in both modes of infection (P<0.01).Antibodies of H9N2 subtype AIV began to be developed on the 6th day and showed an upward trend.The transmission ability of SD18 strain in guinea pigs was verified.It was found that SD18 strain had the ability to infect guinea pigs through direct contact and droplet transmission,but the generation and transmission of aerosols had not been confirmed.When human bronchial epithelial BEAS-2B cells were infected with SD18 strain,the virus could be detected at 12 h after infection,its replicability within cells peaked at 24 h,and the expression of immune factors TLR3,TLR7,MX,OAS,interleukin 6 (IL-6),IL-8 and interferon β(IFN-β) were extremely significantly upregulated (P<0.01).【Conclusion】 The interspecies transmission of SD18 strain had considerably become stronger,it could directly infect guinea pigs without passage adaptation.SD18 strain had the ability to infect guinea pigs and produce antibodies through droplets,and SD18 strain could replicate on human bronchial epithelial BEAS-2B cells.

Key words: H9N2 subtype AIV; lung-delivered aerosol infection; pathogenicity; droplet transmission

CLC Number:

S852.65^＋7

HOU Xiaohong, WANG Meng, WU Bo, LONG Xianrong, ZHAO Xiaomin, GAO Jing, DAI Peiqiang, CHAI Tongjie. Infectivity of H9N2 Subtype Avian Influenza Virus SD18 Strain and Comparison of the Effects of Different Routes of Infection[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1449-1460.

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Infectivity of H9N2 Subtype Avian Influenza Virus SD18 Strain and Comparison of the Effects of Different Routes of Infection

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