禽腺病毒4型云南株分离鉴定及Hexon基因序列分析

doi:10.16431/j.cnki.1671-7236.2022.10.026

Abstract

Abstract: 【Objective】 This study was aimed to analyze the prevalence,genetic difference of Hexon gene and pathogenicity of Fowl adenovirus-4 (FAdV-4) in Yunnan province,so as to provide reference for the epidemiology of FAdV-4 and the influence of Hexon gene on virus virulence.【Method】 158 samples of poultry liver tissue suspected of FAdV-4 infection and 310 fresh feces of wild birds were collected from January 2020 to June 2021 in Kunming,Qujing,Chuxiong,Dali,Honghe and Yuxi of Yunnan province,FAdV-4 nucleic acid was detected by PCR,representative positive samples were isolated and identified,and pathogenic and Hexon gene sequence of isolated strains were analyzed.【Result】 In 158 poultry tissue samples,19 samples were positive for FAdV-4 pathogenic nucleic acid,with a positive rate of 12.03%,and 1 sample (Black-necked cranes) was positive for 310 fresh feces of wild birds,with a positive rate of 0.3%.7 strains of FAdV-4 were isolated from positive samples.The results of pathogenicity study showed that all strains of FAdV-4 in Yunnan could cause chicken embryo dysplasia and pericardial effusion-hepatitis syndrome in 4-week broilers.The embryo median lethal dose (ELD₅₀) was 10^―6.17 to 10^―4.32/mL.The 7 strains were clustered in the branches of FAdV-C subgroup,and Hexon protein had the same characteristics of ¹⁸⁸R,¹⁹³R and ¹⁹⁵Q as domestic highly pathogenic strains.The strains infected with SPF chickens showed obvious clinical symptoms,highly pathogenic,high lethality and significant hepatocellular lesions.【Conclusion】 The prevalence of FAdV-4 in Yunnan was preliminarily understood,FAdV-4 pathogenic nucleic acid was detected in the feces of Black-necked cranes for the first time,7 strains of FAdV-4 were isolated,and some strains of Hexon protein had amino acid mutations.

Key words: Fowl adenovirus-4 (FAdV-4); Hexon gene; pathogenicity; genetic evolution

CLC Number:

S852.65⁺7

XUE Xiaoyan, ZHANG Zhenxing, ZENG Yi, JI Jia, DAI Hongyang, LI Suhua, SONG Jianling. Isolation and Identification of Yunnan Fowl Adenovirus-4 and Sequence Analysis of Hexon Gene[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3932-3942.

References

[1] CHEN L,YIN L,ZHOU Q,et al.Epidemiological investigation of Fowl adenovirus infections in poultry in China during 2015-2018[J].BMC Veterinary Research,2019,15(1):271-275.
[2] KUMAR R,KUMAR V,ASTHANA M,et al.Isolation and identification of a Fowl adenovirus from wild Black kites (Milvus migrans)[J].Journal of Wildlife Diseases,2010,46(1):272-276.
[3] MANZOOR S,HUSSAIN Z,RAHMAN S U,et al.Identification of antibodies against hydropericardium syndrome in wild birds[J].British Poultry Science,2013,54(3):325-328.
[4] 卢琴,罗青平,张腾飞,等.鹌鹑源FAdV4 Fiber 2、ORF29区序列分析及其致病性[J].中国动物传染病学报,2020,35(7):1-9. LU Q,LUO Q P,ZHANG T F,et al.Genomics and pathogenicity analysis of Avian adenovirus type 4 derived from quail[J].Chinese Journal of Animal Infectious Diseases,2020,35(7):1-9.(in Chinese)
[5] LI C,LI H,WANG D,et al.Characterization of Fowl adenoviruses isolated between 2007 and 2014 in China[J].Veterinary Microbiology,2016,197:62-67.
[6] 宋文平,李雪峰,秦亚萍,等.鸡腺病毒血清4型Hexon蛋白的表达及与不同血清型抗体的交叉反应性评价[J].中国兽医科学,2020,50(6):679-688. SONG W P,LI X F,QIN Y P,et al.Expression of Hexon protein of Fowl adenovirus serotype 4 and the cross-reaction with antibodies of different serotypes[J].China Veterinary Science,2020,50(6):679-688.(in Chinese)
[7] 李允章,梅楠,汪凯,等.血清4型鸡腺病毒AHHN分离株全基因组测序及其致病性研究[J].中国预防兽医学报,2019,41(6):637-640. LI Y Z,MEI N,WANG K,et al.Whole genome sequencing and pathogenicity of group Ⅰ Fowl adenovirus serotype 4 strain AHHN[J]. Chinese Journal of Preventive Veterinary Medicine,2019,41(6):637-640.(in Chinese)
[8] 王晓语.辽宁地区两株FAdV-4分离鉴定及Penton、Fiber1和Fiber2基因遗传进化分析[D].沈阳:沈阳农业大学,2019. WANG X Y.The isolation identification of two strain Fowl adenovirus serotype 4 and the phylogenetic analysis of Penton,Fiber1 and Fiber2 genes in Liaoning area[D].Shengyang:Shenyang Agricultural University,2019.(in Chinese)
[9] 朱丽华,王超,宋建领.2016~2020年云南部分地区禽腺病毒4型检测分析[J].中国畜禽种业,2020,16(10):17-19. ZHU L H,WANG C,SONG J L.Analysis of Fowl adenovirus serotype 4 detection in Yunnan parts of from 2016 to 2020[J].The Chinese Livestock and Poultry Breeding Industry,2020,16(10):17-19.(in Chinese)
[10] MATCZUK A K,NICZYPORUK J S,KUCZKOWSKI M,et al.Whole genome sequencing of Fowl aviadenovirus A—A causative agent of gizzard erosion and ulceration,in adult laying hens[J].Infection Genetics and Evolution,2017,48(2):47-53.
[11] SHEPPARD M,MCCOY R J,WERNER W.Genomic mapping and sequence analysis of the Fowl adenovirus serotype 10 Hexon gene[J].Journal of General Virology,1995,76(10):2595-2600.
[12] 冯敬敬,牛登云,马利芳,等.2015—2020年我国禽腺病毒流行病学调查及高致病性FAdV-4分离株遗传变异性分析[J].中国动物传染病学报,2021,29(5):92-98. FENG J J,NIU D Y,MA L F,et al.Epidemiological survey of Avian adenovirus in China from 2015 to 2020 and analysis of genetic variability of highly pathogenic FAdV-4 isolates[J]. Chinese Journal of Animal Infectious Diseases,2021,29(5):92-98.(in Chinese)
[13] ZHANG Y,LIU A,WANG Y,et al.A single amino acid at residue 188 of Hexon protein is responsible for the pathogenicity of the emerging novel Fowl adenovirus 4[J].Journal of Virology,2021,95(17):e0060321.
[14] 金前跃,王寅彪,柴永笑,等.禽腺病毒4型ZZ株的致病性研究[J].畜牧与兽医,2021,53(5):55-60. JIN Q Y,WANG Y B,CHAI Y X,et al.Pathogenicity of a Fowl adenovirus serotype 4 isolate ZZ[J].Animal Husbandry & Veterinary Medicine,2021,53(5):55-60.(in Chinese)
[15] 迟兰,任士飞,李心海,等.鸡胚不同接种途径对鸡安卡拉病毒徐州株增殖的影响[J].黑龙江畜牧兽医,2020,3:81-83. CHI L,REN S F,LI X H,et al.Effect of different inoculation routes of chicken embryos on the proliferation of chicken Ankara virus in Xuzhou strain[J]. Heilongjiang Animal Science and Veterinary Medicine,2020,3:81-83.(in Chinese)
[16] 兰虹,任广彩,叶俊贤,等.Ⅰ群禽腺病毒血清11型的分离鉴定及致病性试验[J].中国畜牧兽医,2022,49(3):1067-1076. LAN H,REN G C,YE J X,et al.Isolation,identification and pathogenicity of Fowl adenovirus group Ⅰ serotype 11[J].China Animal Husbandry & Veterinary Medicine,2022,49(3):1067-1076.(in Chinese)
[17] HESS M.Detection and differentiation of Avian adenoviruses:A review[J]. Avian Pathology,2000,29(3):195-206.
[18] 窦砚国,郑肖,陈浩,等.鸡源血清4型禽腺病毒的分离鉴定[J].中国兽医学报,2017,37(6):1036-1040. DOU Y G,ZHENG X,CHEN H,et al.Isolation and identification of the Fowl adenovirus serotype 4 from chicken[J].Chinese Journal of Veterinary Science,2017,37(6):1036-1040.(in Chinese)
[19] WEI Z P,LIU H,DIAO Y J,et al.Pathogenicity of Fowl adenovirus(FAdV) serotype 4 strain SDJN in Taizhou geese[J]. Avian Pathology,2019,48(5):477-485.
[20] 赵玉杰,刘建勋,陈田田,等.血清4型禽腺病毒W株的分离鉴定和全基因组序列分析[J].中国畜牧兽医,2019,46(7):2059-2068. ZHAO Y J,LIU J X,CHEN T T,et al.Isolation,identification and complete genome sequence analysis of Fowl adenovirus genotype 4 W strain[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):2059-2068.(in Chinese)
[21] 潘青.新发血清4型禽腺病毒遗传进化分析及细胞受体的鉴定[D].北京:中国农业科学院,2020. PAN Q.Genetic evolution analysis and cell receptors identification of the emerging serotype 4 Fowl adenovirus[D].Beijing:Chinese Academy of Agricultural Sciences,2020.(in Chinese)
[22] NORRBY E.The relationship between the soluble antigens and the virion of Adenovirus type 3.Ⅰ.Morphological characteristics[J]. Virology,1966,28(2):236-248.
[23] VERA P F,MORALES A,CORTES D V,et al.Clinicopathological characterization and genomic sequence differences observed in a highly virulent Fowl aviadenovirus serotype 4[J]. Avian Pathology,2016,45(1):73-81.
[24] ZHANG Y,LIU R,TIAN K,et al.Fiber2 and Hexon genes are closely associated with the virulence of the emerging and highly pathogenic Fowl adenovirus 4[J].Emerging Microbes & Infection, 2018,7(1):199.
[25] 侯力丹,王嘉,刘丹,等.血清4型禽腺病毒强毒株GY株的分离鉴定和致病性研究[J].中国兽药杂志,2021,55(4):1-10. HOU L D,WANG J,LIU D,et al.Isolation,identification and pathogenicity study of Fowl adenovirus serotype 4 GY strain[J].Chinese Journal of Veterinary Drug,2021,55(4):1-10.(in Chinese)

Isolation and Identification of Yunnan Fowl Adenovirus-4 and Sequence Analysis of Hexon Gene

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