基于RAD-Seq技术的大围山微型鸡遗传进化分析

doi:10.16431/j.cnki.1671-7236.2022.04.021

Abstract

Abstract: 【Objective】 This study was aimed to explore the genetic evolution of Daweishan Mini chicken at molecular level.【Method】 Daweishan Mini chicken and other 18 indigenous chicken breeds,as well as two introduced breeds were used as research objects.10 roosters and 20 hens were selected from each breed.The whole genome DNA was extracted from blood samples,and simplified genome sequencing(RAD-Seq) was carried out to identify genome SNP of 21 breeds.Analysis of genetic structure and diversity of Daweishan Mini chicken,Calculate the genetic statistics,analyze genetic diversity and structure,screen selected genes and perform functional enrichment analysis.【Result】 The results showed that 331 892 SNPs markers were identified in Daweishan Mini chicken.The population genetic analysis indicated that Daweishan Mini chicken possessed moderate genetic diversity,with the average heterozygosity (Ho,0.219) and nucleotide diversity (Pi,0.245),inbreeding coefficient(Fis,0.107).The results of cluster analysis showed that Daweishan Mini chicken had closer genetical affinity with Piao chicken,Chahua chicken and Tibetan chicken.Daweishan Mini chicken had the lowest Fst(0.0929) with Piao chicken and the highest Fst(0.2179) with Henan Game chicken,which indicated that Daweishan Mini chicken had the closest relationship with Piao chicken and farthest genetic relationship with Game chicken.A total of 200 selected genes were screened out,GO analysis showed that these selected genes were mainly enriched in the biological processes of locomotion,stimulus response and signal transduction.KEGG analysis showed that these genes were mainly enriched in signaling pathways including metabolism,regulation of actin cytoskeleton and MAPK.【Conclusion】 The genetic diversity of Daweishan Mini chicken was moderately polymorphic,and it was closely related to Piao chicken.200 selected genes were screened,which would play roles in metabolism,signal transduction and skull development.

Key words: Daweishan Mini chicken; simplified genome sequencing(RAD-Seq); genetic evolution

CLC Number:

S831.2

ZHANG Huiyong, LI Guohui, XUE Qian, ZHOU Chenghao, YIN Jianmei, SU Yijun, XIA Shuli, HAN Wei. Study on Genetic Evolution Mechanism of Daweishan Mini Chicken Based on RAD-Seq Technology[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1393-1401.

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Study on Genetic Evolution Mechanism of Daweishan Mini Chicken Based on RAD-Seq Technology

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