广东省4株PCV2型分离株全基因组进化分析

doi:10.16431/j.cnki.1671-7236.2022.03.023

Abstract

Abstract: 【Objective】 This study was aimed to investigate the genotypes and evolutionary characteristics of Porcine circovirus type 2 (PCV2) isolated from Guangdong province, and provide reference for PCV2 prevention and control and vaccine strain screening in Guangdong province.【Method】 PCV2 whole genome sequences from 4 positive samples were amplified by PCR, and then sequenced and phylogenetically analyzed.MegAlign software was used to analyze the key variation of ORF2 amino acid sequences of 4 Guangdong isolates of PCV2 and reference strains at home and abroad.The capsid protein encoded by ORF2 gene of 4 Guangdong isolates of PCV2 and 4 vaccine strains were predicted by Jameson-Wolf method of DNAStar Protean software.【Result】 The sequence length of 4 Guangdong isolates of PCV2 was 1 767 bp.Phylogenetic tree analysis showed that all the 4 isolates belonged to PCV2d genotype with nucleotide homology of 97.7%-99.1%, 91.7%-99.8% with 54 reference strains at home and abroad, PhuTho/G40312/2018 (Viet Nam, accession No.:LC602996), QZ1410 (Jiangsu, accession No.:MG732832) and GXBB1501211 (Guangxi, accession No.:MH756609) had similar genetic relationships.Analysis of key amino acid variation showed that there were 8 amino acid specific mutation sites were found in Cap protein, which were Y3C, F8Y, T56S, R116K, V123I, K164E, R169G and T216A.Antigen analysis showed that the Cap protein antigenic index of Guangdong isolates was significantly different from that of the four vaccine strains, mainly in the amino acid regions of 7-12, 47-53, 80-90, 160-170 and 205-213.【Conclusion】 In this study, 4 strains of PCV2 isolated from parts of Guangdong province were all 2d subtypes, and there were many mutation sites in the amino acid sequence of Cap protein, and the antigenic index was also significantly different from that of the vaccine strain, suggesting that the epidemic trend of PCV2 in Guangdong province was gradually dominated by 2d subtypes.

Key words: Porcine circovirus type 2(PCV2); genotype; ORF2 gene; antigen index; genetic evolutionary analysis

CLC Number:

S852.65

YANG Lulu, LI Anqi, YUAN Sheng, HUANG Liangzong, HUANG Shujian, WEN Feng, GUO Jinyue. Complete Genome Evolution Analysis of Four Porcine Circovirus Type 2 Isolates from Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1015-1023.

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Complete Genome Evolution Analysis of Four Porcine Circovirus Type 2 Isolates from Guangdong Province

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