2017-2019年山东省部分地区猪瘟流行病学调查

doi:10.16431/j.cnki.1671-7236.2020.06.027

Abstract

Abstract: To investigate the molecular epidemiology and genetic diversity of CSFV in Shandong province,1 687 tissue samples collected from January 2017 to December 2019 from suspected pigs in 10 regions (including Jinan,Taian,Liaocheng,Qingdao cities and so on) of Shandong province were detected for CSFV,the CSFV-positive samples were amplified and sequenced for E2 and CSFV gene,and the bioinformatics software DNAStar and Mega 7.0 were used to analyze the genetic evolution of CSFV E2 protein and the complete gene compared with reference strain.In addition,4 866 serum samples collected from the same regions as tissue samples were tested for E2-ELISA antibodies.The results showed that 188 out of 1 687 cases were positive for CSFV,with a total positive rate of 11.1%,and the annually positive rates from 2017 to 2019 were 8.5%,13.1% and 15.1%,respectively,it could be clearly seen that the antigen-positive rate had been increasing year by year,which might indicate that there was a certain risk and pressure in the immunization of swine fever rabbit attenuated vaccine in large-scale swine farms.While 4 146 of 4 866 blood samples (85.2%) were positive for E2 antibody,and the annually positive rates of E2 antibodies from 2017 to 2019 were 84.3%,83.0% and 92.3%,respectively.The positive rate of antibody detection had increased year by year,indicating that the overall level of swine fever vaccine protection in large-scale farms in Shandong province had increased year by year.In addition,5 strains of CSFV genome and 5 strains of virus E2 gene sequences were obtained from the positive disease material.The sequence homology between the 5 clinical isolates and the 2.1d subtype CSFV genome were 97.5% to 99.5%,indicating that the 5 isolates were closely related to the CSFV of the 2.1d subgenotype,mutations in the amino acid sites of the isolated strains were at positions 102 (L→M) and 159 (K→R).This study analyzed the prevalence and variation of CSFV in pigs in parts of Shandong from January 2017 to December 2019,and the results could provide a reference for the prevention and control in Shandong.

Key words: classical swine fever virus(CSFV); molecular epidemiology; complete genome; E2 gene; 2.1d subgenotype

CLC Number:

S852.65⁺1

WANG Jiayun, WANG Xin, WANG Bingyu, ZENG Hao, SHEN Wenhui, YU Jiang, CHEN Zhi, ZHANG Yuyu, SUN Wenbo, GUO Lihui, REN Sufang, LI Yubao, WU Jiaqiang. Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1870-1881.

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Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019

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