成都地区宠物犬感染犬瘟热病毒和犬呼吸道冠状病毒的分子流行病学调查

doi:10.16431/j.cnki.1671-7236.2018.02.025

Abstract

Abstract:

In order to investigate the prevalence and distribution of canine distemper virus (CDV) and canine respiratory coronavirus (CRCoV) infections in pet dogs in Chengdu area, 420 samples of nasal cotton swabs were collected from dogs with respiratory signs in eight animal hospitals in Chengdu area, and the molecular detection were carried out using the RT-PCR assay. From 420 samples, 213 samples were detected as CDV positive with the positive rate of 50.71%, and 247 samples were detected as CRCoV positive with the positive rate of 58.81%; The positive rate of mixed detection of CDV and CRCoV was 41.19%. The results indicated that the infection of CDV and CRCoV in pet dogs in Chengdu area was serious, and the mixed infection rate was also high. The detection rate of CDV and CRCoV in pet dogs was different from age, sex, variety, season and immunity. The detection rates of CDV and CRCoV in 1 to 3 month-old puppies were the highest, up to 74.40% and 79.20%, respectively; And in purebred dogs were 59.37% and 62.22%, respectively, which were higher than other varieties; The detection rate of CDV was higher up to 56.19% in spring, however the detection rate of CRCoV was higher up to 67.59% in winter. The detection rates of CDV and CRCoV in non-vaccinated dogs were higher than other dogs which were 64.42% and 63.46%, respectively. This study enriched the epidemiological data of CDV and CRCoV in pet dogs,and provided basic data for diagnosis and control of CDV and CRCoV infections in pet dogs in Chengdu area.

Key words: canine infectious respiratory disease (CIRD); canine distemper virus (CDV); canine respiratory coronavirus (CRCoV); molecular epidemiology

CLC Number:

S858.292

ZHANGX Xin, HUANG Jian, ZHANG Ping, YANG Xiaonong. Molecular Epidemiologic Investigation on Infections of Canine Distemper Virus and Canine Respiratory Coronavirus in Pet Dogs in Chengdu Area[J]. , 2018, 45(2): 486-492.

References

[1] PRIESTNALL S L, MITCHELL J A, WALKER C A, et al. New and emerging pathogens in canine infectious respiratory disease[J]. Veterinary Pathology, 2014, 51(2):492-504.
[2] DALY J M, BLUNDEN A S, MACRAES, et al. Transmission of equine influenza virus to English foxhounds[J]. Emerging Infectious Diseases, 2008, 14(3):461-464.
[3] PECORARO H L, BENNETT S, HUYVAERT K P, et al. Epidemiology and ecology of H3N8 canine influenza viruses in US shelter dogs[J]. Journal of Veterinary Internal Medicine, 2014, 28(2):311-318.
[4] 王西西, 王凤雪, 程世鹏, 等. 犬瘟热病毒感染机制及其诊断方法研究进展[J], 中国畜牧兽医, 2015, 42(3):757-763.WANG X X, WANG F X, CHENG S P, et al. Research progress on infection mechanism and diagnostic methods of canine distemper virus[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(3):757-763. (in Chinese)
[5] 陈秀. 犬瘟热流行病学调查与防制措施[J]. 中国畜牧兽医, 2012, 39(12):169-172.CHEN X. Epidemiological survey and prevention measures of canine distemper[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(12):169-172. (in Chinese)
[6] 苏凤艳, 李哲, 刘千辉, 等. 犬瘟热诊断技术的研究进展[J].中国畜牧兽医, 2015, 42(5):1318-1324.SUN F Y, LI Z, LIU Q H, et al. Research progress on diagnostic technique of canine distemper[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(5):1318-1324. (in Chinese)
[7] MELI M L,SIMMLER P,CARRORI V, et al. Importance of canine distemper virus (CDV) infection in free-ranging Iberian lynxes (Lynx pardinus)[J]. Veterinary Microbiology, 2010, 146(1-2):132-139.
[8] BEINEKE A, MARKUS S, BORLAK J, et al. Increase of pro-inflammatory cytokine expression innon-demyelinating early cerebral lesions in nervous canine distemper[J]. Viral Immunology, 2008,21(4):401-410.
[9] LAN N T, YAMAGUCHI R, KIEN T T, et al. First isolation and characterization of canine distemper virus in Vietnam with the immunohistochemical examination of the dog[J]. Journal of Veterinary Medical Science, 2009, 71(2):155-162.
[10] TAKAYAMA I, KUBO M, TAKENAKA A, et al. Pathological and phylogenetic features of prevalent canine distemper viruses in wild masked palm civets in Japan[J]. Comparative Immunology Microbiology and Infectious Diseases, 2009, 32(6):539-549.
[11] ALSOLAMY S. Middle East respiratory syndrome:Knowledge to date[J]. Critical Care Medicine, 2015, 43(6):1283-1290.
[12] VⅡTANEN S J, LAPPALAINEN A, RAJAMÄKI M M. Co-infections with respiratory viruses in dogs with bacterial pneumonia[J]. Journal of Veterinary Internal Medicine, 2015, 29(2):544-551.
[13] MITCHELL J A, BROOKS H K, SZLADOVITS B, et al. Tropism and pathological findings associated with canine respiratory coronavirus (CRCoV)[J]. Veterinary Microbiology, 2013, 162(2-4):582-594.
[14] MITCHELL J A, BROOKS H, SHIU K B, et al. Development of a quantitative Real-time PCR for the detection of canine respiratory coronavirus[J]. Veterinary Microbiology, 2009, 155(2):136-142.
[15] DECARO N, DESARIO C, ELIA G, et al. Serological and molecular evidence that canine respiratory coronavirus is circulating in Italy[J]. Veterinary Microbiology, 2007, 121(3-4):225-230.
[16] KANESHIMA T, HOHDATSU T, SATOH K, et al. The prevalence of a group 2 coronavirus in dogs in Japan[J].Journal of Veterinary Medical Science, 2006, 68(1):21-25.
[17] KNESL O, ALLAN F J, SHIELDS S. The seroprevalence of canine respiratory coronavirus and canine influenza virus in dogs in New Zealand[J]. New Zealand Veterinary Journal, 2009, 57(5):295-298.
[18] PRIESTNALL S L. BROWMLIE J, DUBOVI E J, et al. Serological prevalence of canine respiratory coronavirus[J]. Veterinary Microbiology, 2006, 115(1-3):43-53.
[19] 肖圆, 吕艳丽. 犬呼吸道冠状病毒研究进展[J].动物医学进展, 201637(9):91-94.XIAO Y, LV Y L. Progress on canine respiratory coronavirus[J]. Progress in Veterinary Medicine, 201637(9):91-94. (in Chinese)
[20] JEOUNG H Y, Song D S, JEONG W S, et al. Simultaneous detection of canine respiratory disease associated viruses by a multiplex reverse transcription-polymerase chain reaction assay[J]. Journal of Veterinary Medical Science, 2012, 8(22):103-106.
[21] 李鑫, 杨霞. 菏泽市犬瘟热发病调查[J]. 黑龙江农业科学, 2014,4:68-70.LI X, YANG X. Incidence investigation of canine distemper in Heze[J]. Heilongjiang Agricultural Sciences, 2014,4:68-70. (in Chinese)
[22] 杨颖, 王芳蕊, 于海霞. 天津地区宠物狗犬瘟热流行病学调查[J]. 黑龙江畜牧兽医, 2015,18:183-185.YANG Y, WANG F R, YU H X. Epidemiological survey of canine distemper in pet dogs in Tianjin area[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015,18:183-185. (in Chinese)
[23] 马丽, 彭广能. 犬瘟热流行病学调查[J]. 中国兽医志, 2007, 43(10):88-89.MA L, PENG G N. Epidemiological survey of canine distemper[J]. Chinese Journal of Veterinary Medicine, 2007, 43(10):88-89. (in Chinese)
[24] 张艳雯, 郑一民. 南宁地区犬瘟热流行病学调查[J].黑龙江畜牧兽医, 2015,11:175-177.ZHANG Y W, ZHENG Y M. Epidemiological survey of canine distemper in Nanning area[J]. Heilongjiang Animal Science and Veterinary Medicine, 2015,11:175-177. (in Chinese)
[25] AN D J, JEOUNG H Y, JEONG W, et al. A serological survey of canine respiratory coronavirus and canine influenza virus in Korean dogs[J]. Journal of Veterinary Medical Science, 2010, 3(30):1217-1219.
[26] ERLES K, TOOMEY C, BOOKS H W, et al. Detection of a group 2 coronavirus in dogs with canine infectious respiratory disease[J]. Virology, 2003,310(2):216-223.
[27] MITCHELL J A, BROWNLIE J. The challenges in developing effective canine infectious respiratory disease vaccines[J]. Journal of Pharmacy and Pharmcology, 2015, 57(3):372-381.
[28] ERLES K, BROENLIE J. Investigation into the causes of canine infectious respiratory disease:Antibody responses to canine respiratory coronavirus and canine herpesvirus in two kennelled dog populations[J]. Archives Virology, 2005, 150(8):1493-1504.
[29] SCHULZ B S, KURZ S, WEBER K, et al. Detection of respiratory viruses and bordetella bronchiseptica in dogs with acute respiratory tract infections[J]. Veterinary Journal, 2014, 201(3):365-369.
[30] JOFFE D J, LELEWSKI R, WEESE J S, et al. Factors associated with development of canine infectious respiratory disease complex (CIRD) in dog in 5 Canadian small animal clinics[J]. Canadian Veterinary Journal-Revue Veterinaire Canadienne, 2016, 57(1):46-51.

Molecular Epidemiologic Investigation on Infections of Canine Distemper Virus and Canine Respiratory Coronavirus in Pet Dogs in Chengdu Area

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[2]	ZHAO Jing, CHU Ying, LUO Jun, GUO Xiaofeng. Construction and Immunogenicity of Recombinant Rabies Virus with Canine Distemper Virus H Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4776-4785.
[3]	LIU Jian, BAI Yilan, JU Houbin, PAN Zihao, YU Xiangqian, YANG Xianchao, ZHU Xiaoying, GE Feifei, GE Jie, YU Huiru, TAO Tiangusheng, WANG Jian, ZHAO Hongjin. Genomic Sequencing Analysis of SH202003 Strain of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2559-2568.
[4]	WANG Zhaoyang, JIANG Yajun, LIU Xueting, LIN Weidong, XIN Ting, HOU Shaohua, GUO Xiaoyu, ZHU Hongfei, JIA Hong. Construction and Screening of Phage Library Against VHH Antibody of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1685-1693.
[5]	WANG Jiayun, WANG Xin, WANG Bingyu, ZENG Hao, SHEN Wenhui, YU Jiang, CHEN Zhi, ZHANG Yuyu, SUN Wenbo, GUO Lihui, REN Sufang, LI Yubao, WU Jiaqiang. Epidemiological Investigation of CSFV in Shandong Province from 2017 to 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1870-1881.
[6]	LIU Hanyang, ZHANG Yi, XIAO Ran, CHEN Bin, WU Yongsheng, SU Zhipeng, LI Yupeng, PEI Chaoxin, XU Zhenying, ZHU Jiawen. Molecular Epidemiological Investigation on ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in Sichuan in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1172-1182.
[7]	YU Zekun, DUAN Xiaoxiao, ZHI Yong, LUAN Zhifang, SHAN Xueqiang. Construction of Vero Cell Line Stably Expressing Nectin4 Receptor of Canine Distemper Virus [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3334-3342.
[8]	JIANG Xinhua, ZHOU Hetian, LUO Feng, YANG Danfeng, ZHANG Zhiqing, CHEN Songchang, DENG Shunzhou. Molecular Epidemiological Investigation of Porcine Epidemic Diarrhea Virus in Jiangxi Province in 2017 [J]. , 2019, 46(5): 1474-1482.
[9]	GUO Yingcheng, FU Shoupeng, GAO Xueyong, LI Xudong. Molecular Epidemiological Investigation and Genetic Evolution Analysis of Porcine Circovirus Type 3 in Jilin Province [J]. , 2019, 46(5): 1483-1490.
[10]	CHEN Meirong, LIN Weidong, SONG Tianqi, XIN Ting, GUO Xiaoyu, HUANG Kehe, JIA Hong. Expression and Identification of H Gene of CDV BJ16B2 Strain in Insect Baculovirus Expression System [J]. , 2018, 45(8): 2041-2048.
[11]	ZHAO Xueli, LIU Yi, BAN Fuguo, YAN Ruoqian, WANG Huajun, WANG Shujuan, MA Zhenyuan, WANG Dongfang. Establishment and Application of A Double TaqMan MGB Real-time PCR Assay for Detection of Canine Distemper Virus and Canine Parvovirus [J]. , 2018, 45(8): 2086-2094.
[12]	YUAN Weifeng, ZHANG Fanfan, LI Longxian, LI Hao, ZHANG Fuhua, LIU Xiaoping, YE Yu, LI Kai, HUANG Dongyan, DING Zhen, WU Qiong, SONG Deping, TANG Yuxin. Molecular Epidemiology Investigation and ORF5 Gene Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Jiangxi Province During 2016 to 2017 [J]. , 2018, 45(10): 2831-2839.
[13]	TUO Xin, LIU Yong-ming, HUANG Mei-zhou, CUI Dong-an, WANG Hui, WANG Sheng-yi, QI Zhi-ming. Research Progress on the Bacterial and Viral Etiology of Lamb Diarrhea [J]. , 2016, 43(3): 831-836.
[14]	LIU Yun-bo, ZHAO Hong-cui, WANG Zhi-cheng, WU Gang, HE Qi-gai. Molecular Epidemiological Investigation of Swine Viral Diarrhea [J]. , 2013, 40(2): 204-207.
[15]	ZHU Xiao-fu;WU Xu-jin;XU De-qian;YANG Ping. The Genome and ORF Protein-coding Structure and Function of Classical Swine Fever Virus [J]. , 2012, 39(5): 183-187.