2016-2017年江西地区猪繁殖与呼吸综合征病毒分子流行病学调查及ORF5基因变异分析

doi:10.16431/j.cnki.1671-7236.2018.10.020

Abstract

Abstract:

To investigate the genetic variation and molecular epidemiology of porcine reproductive and respiratory syndrome virus (PRRSV) in Jiangxi province in the recent years,a total of 453 tissue samples were collected from porcine reproductive and respiratory syndrome (PRRS) suspected cases in pig farms in different regions of Jiangxi province during 2016 to 2017,and then examined by RT-PCR.Of the 453 clinical samples,321 were identified positive for PRRSV,with a positive rate of 70.86%.The positive rates were varying from 19.15% to 84.85% in different regions.14 positive samples were sequenced,and the nucleotide homologies of ORF5 gene were among 83% to 100% for the strains identified in this study,and 59.9% to 98.5% for reference strains of PRRSV.14 sequenced strains all belonged to North America type (genotype Ⅱ) PRRSV in this study,4 strains belonged to the highly pathogenic PRRSV (HP-PRRSV,subtype Ⅰ),3 strains belonged to the classic PRRSV (subtype Ⅱ),3 strains belonged to NADC30-like PRRSV (subtype Ⅲ),and 4 strains belonged to the new subtype of PRRSV (subtype Ⅳ).Sequence analysis on deduced amino acid of GP5 coded by ORF5 gene indicated that the significant variations were observed in three epitopes and two important antigenic regions in PRRSVs belonged to the four subtypes (subtypesⅠ,Ⅱ,Ⅲ and Ⅳ).Subtype specific variations in subtypes Ⅲ and Ⅳ were also observed in amino acid of GP5,which might lead to the variation of antigenecity of GP5 protein.The results indicated that there was a new situation of PRRSV strains circulating in Jiangxi province during 2016 to 2017,with multi-subtypes of strains existed and co-infected in pigs.And the most popular subtype was HP-PRRSV,while there were high rates of newly emerged NADC30-like strains and new subtype strains;It was necessary to continuously monitor the epidemiology of PRRSV,which would be benefit for the development of new diagnostic methods,drugs and vaccines for the prevention and control of PRRS.

Key words: porcine reproductive and respiratory syndrome virus (PRRSV); molecular epidemiology; ORF5 gene; variation analysis

CLC Number:

S852.65

YUAN Weifeng, ZHANG Fanfan, LI Longxian, LI Hao, ZHANG Fuhua, LIU Xiaoping, YE Yu, LI Kai, HUANG Dongyan, DING Zhen, WU Qiong, SONG Deping, TANG Yuxin. Molecular Epidemiology Investigation and ORF5 Gene Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Jiangxi Province During 2016 to 2017[J]. , 2018, 45(10): 2831-2839.

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Molecular Epidemiology Investigation and ORF5 Gene Variation Analysis of Porcine Reproductive and Respiratory Syndrome Virus in Jiangxi Province During 2016 to 2017

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