1株基因Ⅶ型新城疫病毒的生物学特性研究及全基因组序列分析

doi:10.16431/j.cnki.1671-7236.2018.10.021

Abstract

Abstract:

In order to study the genotype,pathogenicity and nucleotide difference between Newcastle disease virus (NDV) isolates and the traditional NDV vaccine strain (La Sota),a suspected NDV virus was isolated from a chicken farm.The isolate was preliminarily determined by HA and HI tests.A pair of primers was designed based on the partial fragment of NDV F gene published at GenBank(accession No.:JF950510.1).F gene was amplified by RT-PCR method,and cloned and sequenced,the sequencing result was compared with the F gene sequences published at GenBank,and the evolutionary tree was constracted to analyze the genotypes.The pathogenicity of the virus was determined by the mean death time (MDT) of chicken embryos,intracerebral pathogenicity index (ICPI) in one-day-old chickens and intravenous pathogenicity index (IVPI) in six-week-old chickens,respectively.Based on the NDV genome sequence published in GenBank(accession No.:JF950510.1),nine pairs of primers were designed to amplify the genome sequence of the isolate strain,and its structure was analyzed.The results showed that the length of F gene was about 500 bp,a NDV strain of genotype Ⅶ was isolated.The MDT,ICPI and IVPI were 52.8 h,1.675 and 2.46,respectively.The whole genome sequence analysis results showed that the full genome length of the isolated strain was 15 192 bp,and had 6 nucleotides more than the strain of La Sota,and the homology between the two strains was 82.8%.Therefore,a virulent NDV strain of genotype Ⅶ was isolated,and contained a large difference in the nucleotide sequence compared with the La Sota strain.

Key words: Newcastle disease virus(NDV); F gene; virulent strain; whole genome

CLC Number:

S852.65⁺9.5

SUN Hualu, BI Yunying, YU Zekun, LI Sifei, LIU Yang, LI Mingyi, CUI Xianlan. Biological Characterization and Complete Genomic Analysis of a Genotype Ⅶ Newcastle Disease Virus Strain[J]. , 2018, 45(10): 2840-2848.

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Biological Characterization and Complete Genomic Analysis of a Genotype Ⅶ Newcastle Disease Virus Strain

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