人工感染鸭源新城疫病毒对绍鸭β-防御素和细胞因子基因表达的影响

doi:10.16431/j.cnki.1671-7236.2019.02.025

Abstract

Abstract:

To investigate the effects of artificial infected duck Newcastle disease virus (NDV) on the expression of avian β-defensins(AvBDs) and cytokines in Chinese Shaoxing duck,forty five 20-day-old SPF Chinese Shaoxing ducks were randomly divided into attack group (27) and control group (18).The ducks in attack group were intraocularly inoculated with 10^8.38 EID₅₀ of virulent NDV strain (Md/CH/LGD/1/2005),others were mock-inoculated with phosphate-buffered saline (PBS).6 ducks in two groups were killed at 24 and 48 h,respectively.Nine tissues including spleen,lung,kidney,glandular stomach,bursa of Fabricius,cecal tonsil,trachea,harderian glands and marrow,were collected from ducks for Real-time quantitative PCR analysis to quantify the mRNA levels of AvBDs and cytokines.In addition,the remaining ducks were constantly observed every day,serum was collected,and specific antibody titres was evaluated at 4,8,12,16,20 and 24 d,respectively.Oropharyngeal and cloacal swabs were collected and specific antibody titres was evaluated at 24,48,72 and 120 h,respectively.The results showed that no obvious clinical signs were observed in the NDV infected ducks.The ducks began to detoxify at the 1st day after attack,and stopped at the 5th day,NDV was detected in the oropharyngeal and cloacal swabs of attack group.The antibody level test results showed that NDV could induce anti-NDV specific antibodies in ducks.Real-time quantitative PCR results showed that compared with control group,the expression levels of AvBD1,AvBD2,AvBD5,AvBD6,AvBD9 and AvBD16 were significantly increased in some tissues of NDV infected ducks at 24 h (P<0.05);The expression levels of AvBD1 and AvBD6 were significantly up-regulated in some tissues of NDV infected ducks at 48 h (P<0.05).The expression levels of IL-6 in bursa of Fabricius and IFN-γ in spleen at 48 h were lower than that at 24 h after infection.In summary,the duck-derived NDV strain infection could induce early natural immune responses in Chinese Shaoxing duck,and these reactions might be related to the replication of the virus in the host.

Key words: Chinese Shaoxing duck; Newcastle disease virus(NDV); avian β-defensins(AvBDs); cytokines

CLC Number:

S852.65⁺9.5

XU Liwen, WANG Qiuling, WANG Fangfang, LIU Chenggang, MA Deying. Effects of Artificially Infected Duck Newcastle Disease Virus on the Expression of β-defensins and Cytokines in Chinese Shaoxing Ducks[J]. , 2019, 46(2): 536-547.

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Effects of Artificially Infected Duck Newcastle Disease Virus on the Expression of β-defensins and Cytokines in Chinese Shaoxing Ducks

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