China Animal Husbandry and Veterinary Medicine ›› 2022, Vol. 49 ›› Issue (2): 731-737.doi: 10.16431/j.cnki.1671-7236.2022.02.035

• Basic Veterinary Medicine • Previous Articles     Next Articles

Effect of Polysaccharide from Rhodomyrtus tomentosa Fruit on Immunological Function in Immunosuppressed Mice

YIN Huihui1, TONG Yanmei2, ZENG Xueyan1, LU Guangqiang3, ZHAO Wu1, JIANG Yuanming1, LIU Wei1   

  1. 1. Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China;
    2. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
    3. Guangxi Longan Center for Animal Disease Control and Prevention, Nanning 532799, China
  • Received:2021-08-16 Online:2022-02-05 Published:2022-01-27

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Abstract: 【Objective】 This study was aimed to investigate the effect of Rhodomyrtus tomentosa fruit polysaccharide on immunological function in immunosuppressed mice.【Method】 Cyclophosphamide was injected intraperitoneally with 80 μg/g BW, once a day, for 3 consecutive days, and mice immunosuppressive model was established.The successfully modeled mice were divided into 5 groups randomly (16 in each group): model group, astragalus polysaccharide group and Rhodomyrtus tomentosa fruit polysaccharide low, medium and high dose group.Another 16 healthy mice were set as normal group.Each mouse in astragalus polysaccharide group was intraperitoneally injected with astragalus polysaccharide suspension at a dose of 200 μg/g BW, and in Rhodomyrtus tomentosa fruit polysaccharide low, medium, and high-dose groups was treated with 50, 100 and 200 μg/g BW Rhodomyrtus tomentosa fruit polysaccharide suspension, respectively.The mice in normal group and model group were treated with normal saline of equal volume.This injection was administered once a day for 7 days.After the test, thymus index, spleen index, the content of IL-1β, IL-2, IL-6, IFN-γ, LZM and POD activity in serum were determined.【Result】 The thymus index and spleen index were increased by different doses of Rhodomyrtus tomentosa fruit polysaccharide, and the differences between the medium dose group and model group were extremely significant (P < 0.01).The contents of IL-6 and IL-1β in different doses of Rhodomyrtus tomentosa fruit polysaccharide group were extremely significantly higher than those in model group (P < 0.01).Compared with model group, the IFN-γ and IL-2 levels in low and medium dose groups increased, but the difference was not significant (P > 0.05).The contents of IFN-γ and IL-2 in high-dose group were extremely significantly higher than those in the model group (P < 0.01).The POD activity and LZM content of mice in different doses of Rhodomyrtus tomentosa fruit polysaccharide groups were extremely significantly or significantly higher than those in the model group (P < 0.01;P < 0.05).【Conclusion】The medium dose of Rhodomyrtus tomentosa fruit polysaccharide (100 μg/g BW) had the best effect on the recovery of immune function in immunosuppressive mice.

Key words: Rhodomyrtus tomentosa fruit polysaccharide; cyclophosphamide; immunomodulatory; cytokines

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