五味止痢合剂对大肠杆菌致小鼠腹泻的治疗作用

doi:10.16431/j.cnki.1671-7236.2022.05.042

Abstract

Abstract: 【Objective】The purpose of this experiment was to study the therapeutic effect of Wuwei Zhili mixture on diarrhea induced by Escherichia coli in mice.【Method】 Four-week-old clean grade ICR mice were divided into blank control (BC),model control (MC),Wuwei Zhili mixture high (W-H),medium (W-M),low (W-L) doses and ciprofloxacin hydrochloride control (CPFX) groups.20 mice in each group were intraperitoneally administered with Escherichia coli except BC group to construct the diarrhea model.When diarrhea symptoms appeared,the treatment group was intragastrically administered with high,medium and low doses of Wuwei Zhili mixture (25,20,15 mL/kg BW,equivalent to crude drug 2.5,2.0,1.5 g/kg BW),CPFX group was intragastrically administered with ciprofloxacin hydrochloride (20 mL/kg BW),and MC group and BC group were intragastrically administered with the same amount of normal saline,twice a day for 5 successive days.Diarrhea rate and protection rate were determined 14 days after drug withdrawal.Another 100 mice were divided and administrated according to the above methods,the duodenum and jejunum were collected on the days 2 after administration and the day 1,7 and 14 after drug withdrawal.The expressions of duodenal mucosa repair factor proliferating cell nuclear antigen (PCNA) and transforming growth factor-α (TGF- α) were detected by immunohistochemistry.【Result】 On the day 14 after drug withdrawal,the protection rates of diarrhea in W-H and W-M groups were 95% and 90%,and the mortality rates were both 0,which was similar to CPFX group.In the course of treatment,the small intestinal villi of mice in W-H and W-M groups gradually tended to be intact,the infiltration of lymphocytes gradually decreased,and the mucosal morphology gradually tended to be normal.The sIgA contents in W-H,W-M and CPFX groups were significantly higher than that in MC group (P<0.05).The secretion of IFN-α in intestinal mucosa of W-H,W-M,W-L and CPFX groups were significantly inhibited (P<0.05),and the secretion of IL-4 were significantly increased (P<0.05) when compared to that of MC group at day 1,7 and 14 after drug withdrawal.On the day 14 after drug withdrawal,the contents of IFN-α and IL-4 in W-H group were similar to that in BC group.On day 1 and 7 after drug withdrawal,the expression level of intestinal repair factor PCNA in W-H and CPFX groups was significantly higher than that in MC group (P<0.05).On day 1 after drug withdrawal,the expression level of intestinal repair factor TGF-α in W-H and CPFX groups was significantly higher than that in MC group (P<0.05),and compared with MC group,there was no significant difference in TGF-α expression in W-H and CPFX groups on day 7 after drug withdrawal (P>0.05).There were no significant differences in the expression of PCNA and TGF-α among all groups on day 14 after drug withdrawal (P>0.05).【Conclusion】 2.5 g/kg BW Wuwei Zhili mixture could significantly control diarrhea caused by Escherichia coli in mice,and the protection rate was 95%.It played a prominent therapeutic role by increasing the content of sIgA in jejunal lavage fluid,fully activating immune cells,regulating the cytokines IFN-α and IL-4 secreted by intestinal mucosa,effectively inhibiting intestinal inflammatory response,stimulating the expression of intestinal mucosal repair factors PCNA and TGF-α.

Key words: Wuwei Zhili mixture; Escherichia coli; diarrhea; cytokines; repair factor

CLC Number:

S854

CHEN Yu, CHEN Xiaolan, YANG Haifeng, LUO Jun, ZHANG Ting, ZUO Haiping, ZHENG Yi. Therapeutic Effect of Wuwei Zhili Mixture on Diarrhea Induced by Escherichia coli in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 2001-2009.

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Therapeutic Effect of Wuwei Zhili Mixture on Diarrhea Induced by Escherichia coli in Mice

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