鸭源致病性大肠杆菌O抗原与毒力基因检测及药敏试验

doi:10.16431/j.cnki.1671-7236.2023.02.029

Abstract

Abstract: 【Objective】 To investigate the O antigen,virulence gene and drug resistance of Escherichia coli (E.coli) isolates from ducks in Rongchang,Dazu and Longchang regions.【Method】 107 strains of bacteria isolated from duck diseased materials from 2014 to 2021 were inoculated in MacConkey medium under sterile conditions for culture and purification,identified by 16S rDNA gene amplification sequencing and biochemical test,detected O antigen and 16 virulence genes by PCR technology,and conducted drug sensitivity test by Kirby-Bauer disk diffusion method.【Result】 107 isolates identified as E.coli.O antigen identification tests identified nine O antigens,of which the dominant antigens were O78 (37.00%),O7 (25.00%),O121 and O145 (both 15.00%),and five O78+O145 and O7+O145 fusion strains were detected.The virulence gene detection test detected 11 virulence factors,of which the strongly pathogenic virulence genes were Tsh gene with a detection rate of 25.23%, fyuA gene with a detection rate of 31.78%, estB gene with a detection rate of 31.78%, Vat gene with a detection rate of 2.80% and iucA gene with a detection rate of 44.56%.3 virulence genes, ompA, yijP and ibeB,had the highest carriage rates of 100.00%,96.26% and 85.98%,respectively.The drug sensitivity test results showed that the isolates were most sensitive to aminoglycosides,minocycline and polymyxin,and resistant to macrolides and clindamycin.All isolates were multi-drug resistant,with 30.00% of isolates showing 7-fold resistance.【Conclusion】 In this study,107 strains of pathogenic E.coli from ducks were identified,which were highly pathogenic and drug resistant,and virulence gene carrier and drug resistance detection found that the virulence of E.coli had a certain relationship with its drug resistance.O antigen detection found five new O antigen fusion strains,the dominant O antigen was constantly changing,indicating that the genetic structure of E.coli in Southwest China was changing,and there might be new bacterial antigens.The results of this study provided a basis for the prevention and control of duck colibacillosis and vaccine preparation in Southwest China.

Key words: Escherichia coli form duck; O-antigen; virulence gene; drug sensitivity test

CLC Number:

Q939.94

LUO Gan, ZOU Hong, REN Shaoke, WU Chunxia, ZOU Yao, LOU Yinying, ZHOU Yang, CHENG Fangjun, GUO Jianhua. Detection of O-antigen,Virulence Gene and Drug Sensitivity Test of Pathogenic Escherichia coli from Duck[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 713-721.

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Detection of O-antigen,Virulence Gene and Drug Sensitivity Test of Pathogenic Escherichia coli from Duck

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