规模化猪场常见呼吸道细菌性病原的流行病学调查

doi:10.16431/j.cnki.1671-7236.2023.06.028

Abstract

Abstract: 【Objective】 The purpose of the test was to understand the infection status of the main bacterial pathogens responsible for porcine respiratory disease complex (PRDC) in large-scale pig farms in China.【Method】 A total of 2 204 samples of lungs, trachea and secretions, spleens and lymph nodes were collected from 647 large-scale pig farms with clinically obvious respiratory symptoms in 26 provinces, cities and autonomous regions of China in 2021.The sample was inoculated into the plate for purification and culture, and then bacteria were identified by morphological observation, Gram staining and PCR identification.In addition, serotype analysis, mixed infection analysis and drug sensitivity test were carried out on isolated Streptococcus suis (SS), Haemophilus parasuis (Hps), Pasteurella multocida (Pm), Bordetella bronchiseptica (Bb) and Actinobacillus pleuropneumoniae (APP).【Result】 The results showed that 647 strains of pathogenic bacteria were isolated and identified.The isolation rate of pathogenic bacteria was 29.35% (647/2 204), including 306 strains of SS (13.88%), 137 strains of Hps (6.22%), 86 strains of Pm (3.90%), 74 strains of Bb (3.35%) and 44 strains of APP (2.00%), respectively.The isolated SS, Hps and APP were chosen to do serotyping tests.The results showed that the major SS was serotype 2, the major Hps was serotype 5, 13 and 4, the major APP was serotype 1.The results of multiple infections showed that the single pathogen infected 407 pig farms, 214 strains of SS, 82 strains of Hps, 38 strains of Pm, 47 strains of Bb and 26 strains of APP were isolated.There were 70 double-mixed infected pig farms, mainly SS+Hps (22 pig farms) and SS+Pm (18 pig farms).There were 6 triple-mixed infected pig farms, mainly SS+Hps+Pm (2 pig farms) and SS+Hps+APP (2 pig farms).The results of drug sensitivity test showed that the isolated bacteria showed different degrees of resistance to 19 kinds of antimicrobial agents, and all the isolated bacteria were resistant to doxycycline and lincomycin, among which Bb showed severe multiple resistance.【Conclusion】 A total of 647 strains of porcine respiratory bacteria pathogens were isolated.SS was mainly serotype 2, Hps was mainly serotype 5, 13 and 4, and APP was mainly serotype 1.The type of multiple infections among different pathogens was complicated, and SS+Hps was the most common type of mixed infection.In this study, different pathogenic bacteria showed different degrees of resistance to 19 kinds of antibiotics.These data could provide a foundation for monitoring epidemiological patterns of bacterial diseases in large-scale pig farms in China, as well as provide reference basis for prevention and control.

Key words: porcine respiratory disease complex (PRDC); serotypes; multiple infection; drug sensitivity test

CLC Number:

S852.61

CAO Qi, JIN Qing, YUAN Yi, FENG Yixue, KONG Danni, CHEN Xianghong, WU Bin, SONG Wenbo, TANG Xibiao. Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2439-2449.

References

[1] BROCKMEIER S L, HALBUR P G, THACKER E L.Polymicrobial Diseases:Porcine Respiratory Disease Complex[M].Washington (DC):ASM Press, 2002.
[2] HALBUR P.Porcine viral respiratory diseases[A].Proceedings of the 14th International Pig Veterinary Society[C].1998.
[3] OPRIESSNIG T, GIMENEZ-LIROLA L G, HALBUR P G.Polymicrobial respiratory disease in pigs[J].Animal Health Research Reviews, 2011, 12(2):133-148.
[4] OPRIESSNIG T, SHEN H G, PAL N, et al.A live-attenuated chimeric Porcine circovirus type 2(PCV2) vaccine is transmitted to contact pigs but is not upregulated by concurrent infection with Porcine parvovirus (PPV) and Porcine reproductive and respiratory syndrome virus (PRRSV) and is efficacious in a PCV2b-PRRSV-PPV challenge model[J].Clinical and Vaccine Immunology, 2011, 18(8):1261-1268.
[5] BROCKMEIER S, REGISTER K, NICHOLSON T, et al.Diseases of Swine:Bordetellosis[M].Chichester:Wiley, 2019.
[6] LIU Z, ZHENG H, GOTTSCHALK M, et al.Development of multiplex PCR assays for the identification of the 33 serotypes of Streptococcus suis[J].PLoS One, 2013, 8(8):e72070.
[7] HOWELL K J, PETERS S E, WANG J, et al.Development of a multiplex PCR Assay for rapid molecular serotyping of Haemophilus parasuis[J].Journal Clinical Microbiology, 2015, 53(12):3812-3821.
[8] TOWNSEND K M, FROST A J, LEE C W, et al.Development of PCR assays for species- and type-specific identification of Pasteurella multocida isolates[J].Journal Clinical Microbiology, 1998, 36(4):1096-1100.
[9] HOZBOR D, FOUQUE F, GUISO N.Detection of Bordetella bronchiseptica by the polymerase chain reaction[J].Research Microbiology, 1999, 150(5):333-341.
[10] BOSSÉ J T, LI Y, FERNANDEZ CRESPO R, et al.Comparative sequence analysis of the capsular polysaccharide loci of Actinobacillus pleuropneumoniae serovars 1-18, and development of two multiplex PCRs for comprehensive capsule typing[J].Veterinary Microbiology, 2018, 220:83-89.
[11] JIA A, ZHOU R, FAN H, et al.Development of serotype-specific PCR assays for typing of Haemophilus parasuis isolates circulating in Southern China[J].Journal Clinical Microbiology, 2017, 55(11):3249-3257.
[12] 陈雨欢, 肖敏, 王雨萌, 等.2017-2019年中国南方五省规模化养猪场副猪格拉菌血清型、耐药性及β-内酰胺类耐药基因bla-_TEM分子特征分析[J].微生物学报, 2022, 62(8):3039-3047. CHEN Y H, XIAO M, WANG Y M, et al.Characterization of serotypes, antibiotic resistance, and bla-_TEM gene of Glaesserella parasuis in large-scale pig farms in 5 southern provinces of China during 2017-2019[J].Acta Microbiologica Sinica, 2022, 62(8):3039-3047.(in Chinese)
[13] GUTIERREZ-MARTIN C B, RODRIGUEZ-DELGADO O, ALVAREZ-NISTAL D, et al.Simultaneous serological evidence of Actinobacillus pleuropneumoniae, PRRS, Aujeszky's disease and Influenza viruses in Spanish finishing pigs[J].Research Veterinary Science, 2000, 68(1):9-13.
[14] HARMS P A, SORDEN S D, HALBUR P G, et al.Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 Porcine circovirus and Porcine reproductive and respiratory syndrome virus[J].Veterinary Pathology, 2001, 38(5):528-539.
[15] ZHANG B, KU X, YU X, et al.Prevalence and antimicrobial susceptibilities of bacterial pathogens in Chinese pig farms from 2013 to 2017[J].Scientific Reports, 2019, 9(1):9908-9918.
[16] SUN Q, YU X, HE D, et al.Investigation and analysis of etiology associated with porcine respiratory disease complex in China from 2017 to 2021[J].Frontiers in Veterinary Science, 2022, 11(9):960033-960046.
[17] 郭龙, 汤细彪, 刘田, 等.2014年猪群常见细菌性疫病的分离鉴定与流行病学调查[J].养殖与饲料, 2015, 10:1-3. GUO L, TANG X B, LIU T, et al.Isolation, identification and epidemiological investigation of common bacterial diseases of swine in 2014[J].Breeding and Feed, 2015, 10:1-3.(in Chinese)
[18] SASSU E, BOSSEJ T, TOBIAS T J, et al.Update on Actinobacillus pleuropneumoniae knowledge, gaps and challenges[J].Transbounday and Emerging Diseases, 2018, 65(1):72-90.
[19] CAO Q, WEI W, WANG H, et al.Cleavage of E-cadherin by porcine respiratory bacterial pathogens facilitates airway epithelial barrier disruption and bacterial paracellular transmigration[J].Virulence, 2021, 12(1):2296-2313.
[20] VÖTSCH D, WILLENBORG M, BAUMGÄRTNER W, et al.Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model[J].Virulence, 2021, 12(1):84-95.
[21] 李红婕, 董紫凡, 车勇良, 等.猪多杀性巴氏杆菌的分离鉴定及oppA基因遗传进化分析[J].中国畜牧兽医, 2022, 49(12):4786-4794. LI H J, DONG Z F, CHE Y L, et al.Isolation and identification of Pasteurella multocida from swine and genetic evolution analysis of oppA gene[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(12):4786-4794.(in Chinese)
[22] YU X, SUN Q, KU X, et al.The epidemiological investigation of co-infection of major respiratory bacteria with Pseudorabies virus in intensive pig farms in China[J].Journal of Veterinary Medical Science, 2021, 7(1):175-183.
[23] CAI X, CHEN H, BLACKALL P J, et al.Serological characterization of Haemophilus parasuis isolates from China[J].Veterinary Microbiology, 2005, 111(3-4):231-236.
[24] 张飞, 曹三杰, 文心田, 等.猪传染性胸膜肺炎疫苗的研究进展[J].中国兽医学报, 2015, 35(10):111-113. ZHANG F, CAO S J, WEN X T, et al.Advance in vaccines against porcine contagious pleuropneumoniae[J].Chinese Journal of Veterinary Medicine, 2015, 35(10):111-113.(in Chinese)
[25] 徐民生, 柯海意, 施科达, 等.广东省猪传染性胸膜肺炎放线杆菌分离鉴定及耐药表型和耐药基因检测与分析[J].中国畜牧兽医, 2022, 49(8):3212-3225. XU M S, KE H Y, SHI K D, et al.Isolation and identification of Actinobacillus pleuropneumoniae from Guangdong province and detection and analysis of drug resistance phenotype and gene[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(8):3212-3225.(in Chinese)
[26] 刘宝玲, 楚品品, 李春玲, 等.猪链球菌临床分离株对四环素类抗生素的耐药性和耐药基因分析[J].中国畜牧兽医, 2022, 49(7):2796-2804. LIU B L, CHU P P, LI C L, et al.Analysis of resistance and resistance genes of Streptococcus suis clinical isolates to tetracycline[J].China Animal Husbandry & Veterinary Medicine, 2022, 49(7):2796-2804.(in Chinese)

Epidemiological Investigation of Common Respiratory Bacterial Pathogens in Large-scale Pig Farms

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