奶牛产气荚膜梭菌的分离鉴定与药物敏感性分析

doi:10.16431/j.cnki.1671-7236.2020.08.028

Abstract

Abstract: The purpose of this study was to isolate and classify Clostridium perfringens (CP) strains from cow viscera and tissue samples to provide materials for subsequent vaccine studies,in addition,sensitive drugs were screened out through drug sensitivity test to guide the pasture to treat cattle and effectively control and isolate cattle without disease.In 2019,some cows in 21 ranches in Shandong province reported diarrhea,hematochezia and short-term death.Some of the dead cows were dissected,and 64 viscera and tissue samples were sent to be cultured for CP preliminary screening,then the positive strains was identified by biochemical identification and PCR typing.The homology of strains was analyzed by 16S rDNA technique.The minimal bacteriostatic concentration of isolated strains against 7 kinds of antibiotics (erythromycin,levfloxacin,linezolid,vancomycin,clindamycin,tetracycline,rifampicin) was determined by E-test method,and sensitive drugs were screened out.8 samples showed gray,orange and black colonies on blood medium,CP chromogenic medium and TSC medium respectively.The biochemical identification results were consistent with the characteristics of CP.PCR typing results showed that 5 A-type strains,2 C-type strains,1 E-type strain.The homology of 8 isolates and Clostridium percapsulatum was up to 100% by 16S rDNA analysis;Among the 8 strains,2 were more sensitive to clindamycin and rifampicin,3 more sensitive to erythromycin and linezolid,1 more sensitive to levfloxacin.Lincoamides,rifamycin and macrolide were recommended for the prevention and control of two farms with diseased cattle.The effect of the prevention and control on the farms was tracked in time.The cattle without therapeutic value were immediately culled and treated innocuously,so as to improve the feeding conditions and reduce the feeding density.

Key words: Clostridium perfringens; isolation and identification; drug sensitivity test; E-test

CLC Number:

S852.61⁺6.3

SUN Yangyang, FAN Xuezheng, YANG Mei, WANG Jilong, QIN Yuming, XIE Xiaoli, YANG Hongjun. Isolation and Identification of Clostridium perfringens from Dairy Cows and Analysis of Drug Sensitivity[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(8): 2588-2595.

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Isolation and Identification of Clostridium perfringens from Dairy Cows and Analysis of Drug Sensitivity

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